Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,772,911	T→C	S134P (TCT→CCT)	ydiN →	putative transporter YdiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,772,911	0	T	C	100.0%	252.7 / NA	72	S134P (TCT→CCT)	ydiN	putative transporter YdiN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/37);  total (35/37)

TCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGTAC  >  NC_000913/1772775‑1773046                                                                                                                                         |                                                                                                                                        tCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTg                                                                                                                                      >  2:247274/1‑140 (MQ=255) tCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTg                                                                                                                                      >  1:554530/1‑140 (MQ=255) tCTGCTATTCCTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTg                                                                                                                                      >  2:659468/1‑140 (MQ=255)  cTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTgg                                                                                                                                     <  1:432664/140‑1 (MQ=255)   tGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGt                                                                                                                                    >  2:232141/1‑140 (MQ=255)   tGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGt                                                                                                                                    >  2:718052/1‑140 (MQ=255)    gCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGtt                                                                                                                                   >  2:672116/1‑140 (MQ=255)    gCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGtt                                                                                                                                   >  1:268364/1‑140 (MQ=255)        ttcttcTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTcggc                                                                                                                               >  2:270928/1‑140 (MQ=255)          cttcttTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTcggcgg                                                                                                                             <  2:268364/140‑1 (MQ=255)                tGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTc                                                                                                                                  <  1:581404/129‑1 (MQ=255)                tGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATAc                                                                                                                       >  2:431061/1‑140 (MQ=255)                tGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTAGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTc                                                                                                                                  >  2:581404/1‑129 (MQ=255)                     ttCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGtt                                                                                                                  >  1:693436/1‑140 (MQ=255)                     ttCCCGCTTGCCCGAACTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGtt                                                                                                                  >  1:51646/1‑140 (MQ=255)                       cccGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTaa                                                                                                                >  1:113651/1‑140 (MQ=255)                       cccGCTTGCCCGAATTTAACTCACGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTaa                                                                                                                >  1:47584/1‑140 (MQ=255)                           cTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCg                                                                                                            <  2:612108/140‑1 (MQ=255)                           cTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCg                                                                                                            <  1:672116/140‑1 (MQ=255)                            ttGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGa                                                                                                           >  2:573674/1‑140 (MQ=255)                               cccGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATgg                                                                                                        <  1:431061/140‑1 (MQ=255)                               cccGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATgg                                                                                                        >  2:144700/1‑140 (MQ=255)                               cccGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATgg                                                                                                        >  1:209856/1‑140 (MQ=255)                                        aaCTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTg                                                                                               >  1:62097/1‑140 (MQ=255)                                         aCTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTgg                                                                                              >  1:635679/1‑140 (MQ=255)                                           tctcGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGc                                                                                            <  1:71248/140‑1 (MQ=255)                                             acGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCaa                                                                                          <  2:47584/139‑1 (MQ=255)                                               gCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAAt                                                                                        <  1:125525/140‑1 (MQ=255)                                                  tACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGtt                                                                                     <  2:554530/140‑1 (MQ=255)                                                  tACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGtt                                                                                     <  2:693436/140‑1 (MQ=255)                                                       tCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTAcc                                                                                <  2:498708/140‑1 (MQ=255)                                                          ggCAGTGTGCGTAGGAATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCaa                                                                             >  2:99879/1‑140 (MQ=255)                                                            cAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATg                                                                           <  2:113651/140‑1 (MQ=255)                                                               tgtgCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTg                                                                        <  2:62097/140‑1 (MQ=255)                                                                 tgCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGt                                                                      <  1:144700/140‑1 (MQ=255)                                                                 tgCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGt                                                                      <  1:247274/140‑1 (MQ=255)                                                                 tgCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGt                                                                      >  1:642731/1‑140 (MQ=255)                                                                    gTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAg                                                                   <  1:718052/140‑1 (MQ=255)                                                                     caGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGc                                                                  >  2:543501/2‑140 (MQ=255)                                                                          aTCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTatat                                                             >  2:117793/1‑140 (MQ=255)                                                                           tCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATg                                                            >  2:614291/1‑140 (MQ=255)                                                                             gCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGt                                                                      <  1:1858/128‑1 (MQ=255)                                                                             gCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGt                                                                      >  2:1858/1‑128 (MQ=255)                                                                               tAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGc                                                        <  1:543501/140‑1 (MQ=255)                                                                                    cAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCaat                                                   <  2:51646/140‑1 (MQ=255)                                                                                     aGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCaata                                                  >  1:312353/1‑140 (MQ=255)                                                                                       cgcTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCaataat                                                >  2:1957/1‑140 (MQ=255)                                                                                         cTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAatat                                              >  1:578507/1‑140 (MQ=255)                                                                                            gATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTg                                           >  1:315895/1‑140 (MQ=255)                                                                                             aTACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTgg                                          <  1:99879/140‑1 (MQ=255)                                                                                               aCGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTa                                        <  1:659468/140‑1 (MQ=255)                                                                                               aCGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTa                                        <  2:635679/140‑1 (MQ=255)                                                                                               aCGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTa                                        <  1:483560/140‑1 (MQ=255)                                                                                                 gggTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACg                                      >  1:9329/1‑140 (MQ=255)                                                                                                    tGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCt                                   <  1:614291/140‑1 (MQ=255)                                                                                                      gCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTAt                                 >  1:537201/1‑140 (MQ=255)                                                                                                      gCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTAt                                 >  1:540870/1‑140 (MQ=255)                                                                                                      gCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATATTGCTCAATAATATCTGGTa                                        <  2:155968/133‑1 (MQ=255)                                                                                                      gCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATATTGCTCAATAATATCTGGTa                                        >  1:155968/1‑133 (MQ=255)                                                                                                        tACCCCGCGCTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATgg                               <  2:642731/140‑1 (MQ=255)                                                                                                                 cTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGattat                      <  2:616946/140‑1 (MQ=255)                                                                                                                 cTCATGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGattat                      >  1:162868/1‑140 (MQ=255)                                                                                                                    aTGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTcc                   <  2:537201/140‑1 (MQ=255)                                                                                                                    aTGGAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTcc                   <  2:312353/140‑1 (MQ=255)                                                                                                                      ggAATGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCgg                 <  2:162868/140‑1 (MQ=255)                                                                                                                          tGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTAt             <  1:270928/140‑1 (MQ=255)                                                                                                                          tGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTAt             <  2:340883/140‑1 (MQ=255)                                                                                                                          tGCTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTAt             <  2:209856/140‑1 (MQ=255)                                                                                                                            cTTTCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTc           >  1:513449/1‑140 (MQ=255)                                                                                                                               tCCGAAAGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTAt        <  1:232141/140‑1 (MQ=255)                                                                                                                                    aaGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGTa   <  2:513449/140‑1 (MQ=255)                                                                                                                                     aGCCCCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGTAc  <  2:9329/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        TCTGCTATTCTTCTTTGGTATTCCCGCTTGCCCGAATTTAACTCTCGCCTACGGTCTGGCAGTGTGCGTAGGTATCGCTAACTCAGCGCTGGATACGGGTGGCTACCCCGCGCTCATGGAATGCTTTCCGAAAGCCTCTGGTTCGGCGGTCATACTGGTTAAAGCGATGGTGTCATTTGGGCAAATGTTCTACCCAATGCTGGTGAGCTATATGTTGCTCAATAATATCTGGTACGGCTATGGGCTGATTATTCCGGGTATTCTATTTGTAC  >  NC_000913/1772775‑1773046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑