Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	132,433	C→T	C273C (TGC→TGT)	acnB →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	132,433	0	C	T	100.0%	92.3 / NA	28	C273C (TGC→TGT)	acnB	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/16);  total (12/16)

GAAAGGTTTCCCGCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACA  >  NC_000913/132298‑132551                                                                                                                                        |                                                                                                                       gAAAGGTTTCCCGCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTcg                                                                                                                    >  1:584909/1‑140 (MQ=255)  aaaGGTTTCCCGCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTcgg                                                                                                                   >  2:286549/1‑140 (MQ=255)    aGGTTTCCCGCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTcggcg                                                                                                                 >  2:247437/1‑140 (MQ=255)         tCCCGCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTaaa                                                                                                            >  2:712101/1‑140 (MQ=255)             gCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTg                                                                                                        <  2:158692/140‑1 (MQ=255)             gCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTg                                                                                                        <  1:247437/140‑1 (MQ=255)                ggCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCAc                                                                                                     <  2:395908/140‑1 (MQ=255)                    tACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCAt                                                                                                 <  1:286549/140‑1 (MQ=255)                     aCGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCAtc                                                                                                <  1:571905/140‑1 (MQ=255)                     aCGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCAtc                                                                                                <  2:212596/140‑1 (MQ=255)                                gTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAacac                                                                                     >  1:366082/1‑140 (MQ=255)                                   gtgGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGGAAAATTTGACCCCTCTTCTTTAACACGAt                                                                                  >  2:316903/1‑140 (MQ=255)                                           gggTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGAcg                                                                          <  2:366082/140‑1 (MQ=255)                                                  tcGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGc                                                                   >  2:420498/1‑140 (MQ=255)                                                                         cGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGtct                                            <  2:430152/140‑1 (MQ=255)                                                                             cTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTaa                                        >  1:368111/1‑140 (MQ=255)                                                                              tgtgGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAAc                                       <  1:712101/140‑1 (MQ=255)                                                                                 ggTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAAATTCACCCACCTTTTTTAACACGATGGAAGACGCGGGGGCACCTCCCAACGGAATCGACGGCTCTAACCtt                                    >  2:313891/1‑139 (MQ=255)                                                                                            gatgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGa                         <  1:578596/140‑1 (MQ=255)                                                                                            gatgatATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGa                         <  1:420498/140‑1 (MQ=255)                                                                                                  atTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGAt                   <  2:368111/140‑1 (MQ=255)                                                                                                   ttCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTTAAACGATGGGAGACGCGGGGGCACTGCCCAACGAAGTCGACGTCTCTAAACCGAAAATGGGCGACGGGAtt                  >  2:194570/1‑140 (MQ=255)                                                                                                         aTGTGCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGtt            <  1:194570/140‑1 (MQ=255)                                                                                                            tgCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTAc         <  1:162969/140‑1 (MQ=255)                                                                                                            tgCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTAc         <  1:316903/140‑1 (MQ=255)                                                                                                             gCCGAACAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTAcc        >  1:246812/1‑140 (MQ=255)                                                                                                                  aCAAACGCGGCGGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGGGGACTGCCAAACGAAGTCGACGTCTCTAACCTGAACAAGGGCGGCGGGATTGACGGTTACCCCTAc   >  2:384964/1‑140 (MQ=255)                                                                                                                   agacagcggcgGTGGTTTGTGTCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACa  <  1:276683/135‑1 (MQ=255)                                                                                                                                        |                                                                                                                       GAAAGGTTTCCCGCTGGCGTACGTCGGTGACGTTGTGGGTACGGGTTCTTCGCGTAAATCCGCCACTAACTCCGTTCTGTGGTTTATGGGCGATGATATTCCACATGTGCCGAACAAACGCGGCGGTGGTTTGTGCCTCGGCGGTAAAATTGCACCCATCTTCTTTAACACGATGGAAGACGCGGGTGCACTGCCAATCGAAGTCGACGTCTCTAACCTGAACATGGGCGACGTGATTGACGTTTACCCGTACA  >  NC_000913/132298‑132551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑