Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,858,333	T→C	K167K (AAA→AAG)	ydjJ ←	putative zinc‑binding dehydrogenase YdjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,858,333	0	T	C	100.0%	142.9 / NA	42	K167K (AAA→AAG)	ydjJ	putative zinc‑binding dehydrogenase YdjJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/21);  total (21/21)

AGCTGTTCCGCCATTGCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGTTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGACACAGATAGTGCGTTAATGCGCCG  >  NC_000913/1858199‑1858468                                                                                                                                       |                                                                                                                                        aGCTGTTCCGCCATTGCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGTACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAAc                                                                                                                                    >  1:716549/1‑140 (MQ=255) aGCTGTTCCGCCATTGCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAAc                                                                                                                                    >  2:234244/1‑140 (MQ=255)         cGCCATTGCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCa                                                                                                                            <  2:700374/140‑1 (MQ=255)            cATTGCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCa                                                                                                                         <  2:594829/140‑1 (MQ=255)                gCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATcgc                                                                                                                     >  1:167926/1‑140 (MQ=255)                 ccAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATcgcg                                                                                                                    <  1:549081/140‑1 (MQ=255)                   aGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGgc                                                                                                                  >  1:646924/1‑140 (MQ=255)                          tttCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATc                                                                                                           >  2:321922/1‑140 (MQ=255)                            tCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATccc                                                                                                         <  2:70571/140‑1 (MQ=255)                                    aTCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGTACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCgg                                                                                                 <  2:716549/140‑1 (MQ=255)                                      cAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCa                                                                                               <  2:721920/140‑1 (MQ=255)                                        acgacgGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAgg                                                                                             <  1:234244/140‑1 (MQ=255)                                                  tttCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAgc                                                                                   <  1:277216/140‑1 (MQ=255)                                                   ttCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAgcg                                                                                  <  1:321922/140‑1 (MQ=255)                                                            tCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCa                                                                         >  1:49761/1‑140 (MQ=255)                                                             cccAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCAt                                                                        >  2:437401/1‑140 (MQ=255)                                                                aGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCgt                                                                     >  1:592535/1‑140 (MQ=255)                                                                aGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCgt                                                                     <  2:646924/140‑1 (MQ=255)                                                                aGGCAGTTGCTCGCTTGCAACGTGATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTGCCATcat                                                                     <  2:167926/140‑3 (MQ=255)                                                                      ttGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCat                                                               >  2:633055/1‑140 (MQ=255)                                                                       tGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCa                                                                <  2:406173/138‑1 (MQ=255)                                                                       tGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCa                                                                >  1:406173/1‑138 (MQ=255)                                                                                  aaCGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAg                                                   <  2:98675/140‑1 (MQ=255)                                                                                       catcaAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgt                                              <  2:49761/140‑1 (MQ=255)                                                                                        atcaAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgt                                              >  1:514696/1‑139 (MQ=255)                                                                                        atcaAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgt                                              >  1:455312/1‑139 (MQ=255)                                                                                        atcaAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgt                                              <  2:455312/139‑1 (MQ=255)                                                                                        atcaAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgt                                              <  2:514696/139‑1 (MQ=255)                                                                                          caAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgtaag                                           >  1:600898/1‑140 (MQ=255)                                                                                            aaCCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTgtaagta                                         >  1:364093/1‑140 (MQ=255)                                                                                                 aTACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGct                                    >  1:687184/1‑140 (MQ=255)                                                                                                   aCAACCTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGctct                                  <  1:633055/140‑1 (MQ=255)                                                                                                       ccTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCgg                              <  2:687184/140‑1 (MQ=255)                                                                                                       ccTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCgg                              <  1:717945/140‑1 (MQ=255)                                                                                                       ccTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCgg                              >  1:373137/1‑140 (MQ=255)                                                                                                       ccTGCTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCgg                              >  1:222207/1‑140 (MQ=255)                                                                                                           cTCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGa                          >  2:103465/1‑140 (MQ=255)                                                                                                            tCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGac                         >  2:704719/1‑140 (MQ=255)                                                                                                            tCCCAGAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCCCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGTGCAGTTTGTAAGTAAAGCTCTCCGGATGac                         >  1:469099/1‑140 (MQ=255)                                                                                                                 gAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGACACAGa                    <  2:222207/140‑1 (MQ=255)                                                                                                                 gAATAATTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGACACAGa                    >  1:88698/1‑140 (MQ=255)                                                                                                                      atTATCTTCTTACCCGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGACACAGATAGTg               <  1:192561/140‑1 (MQ=255)                                                                                                                                   ccGGCTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGACACAGATAGTGCGTTAATGcgccg  <  1:437401/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGCTGTTCCGCCATTGCCAGACGTTTTTCCAGCACATCAACGACGGCAATTTCCGTTGCTCCCAGGCATTTGCACGCTTGCAACGTCATCAAACCAATACAACCTGCTCCCAGAATAATTATCTTCTTACCCGGTTTAACATCTGCCAGCATCGCGGCATGCATCCCGACTGCGGCAGGCTCCACCAGCGCCCCTTCCATCGTGTCCATATTGTCGGGCAGTTTGTAAGTAAAGCTCTCCGGATGACACAGATAGTGCGTTAATGCGCCG  >  NC_000913/1858199‑1858468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑