Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,046,646	T→C	V570A (GTG→GCG)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,046,646	0	T	C	100.0%	92.9 / NA	27	V570A (GTG→GCG)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

CTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAA  >  NC_000913/2046520‑2046778                                                                                                                               |                                                                                                                                     cTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGt                                                                                                                         >  1:464767/1‑140 (MQ=255)   aCGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCa                                                                                                                       >  2:104655/1‑140 (MQ=255)        aaGCCAGAAAGATCCCTCGTTATCGTTAAGTCCCAAAACATTGAACGCGGATCCCCATTCACCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAg                                                                                                                  <  2:115908/140‑1 (MQ=255)              gAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTc                                                                                                            >  1:115908/1‑140 (MQ=255)                   aTTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGAc                                                                                                       >  2:304888/1‑140 (MQ=255)                             aTCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCttt                                                                                             >  2:360421/1‑140 (MQ=255)                               cGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCt                                                                                           <  1:104655/140‑1 (MQ=255)                                      tACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGa                                                                                    >  1:235456/1‑140 (MQ=255)                                       aCCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGaa                                                                                   <  2:235456/140‑1 (MQ=255)                                            aaCATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATa                                                                              >  2:68586/1‑140 (MQ=255)                                            aaCATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATa                                                                              >  2:159425/1‑140 (MQ=255)                                                   aaCGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTgg                                                                                     <  1:281512/126‑1 (MQ=255)                                                   aaCGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTgg                                                                                     >  2:281512/1‑126 (MQ=255)                                                      gcgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                    <  1:159425/140‑1 (MQ=255)                                                       cgGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGa                                                                   >  2:354523/1‑140 (MQ=255)                                                         gATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAg                                                                 <  1:354523/140‑1 (MQ=255)                                                            tCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCta                                                              <  2:464767/140‑1 (MQ=255)                                                                    aaCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                    <  1:312917/126‑1 (MQ=255)                                                                    aaCCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACgg                                                                    >  2:312917/1‑126 (MQ=255)                                                                      ccGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATc                                                    >  1:117572/1‑140 (MQ=255)                                                                                             cATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTgg                             >  2:154293/1‑140 (MQ=255)                                                                                                     agcagGTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCacgctga                     <  1:154293/140‑1 (MQ=255)                                                                                                          gTAATCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCacgctgacgctg                >  2:136628/1‑140 (MQ=255)                                                                                                              tCCTGTTGTCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGc            <  1:136628/140‑1 (MQ=255)                                                                                                                    tgtCGGGCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGACGCCACAGc      >  1:83417/1‑140 (MQ=255)                                                                                                                        gggCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGaa  <  1:68586/140‑1 (MQ=255)                                                                                                                        gggCTGGCGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGaa  <  1:304888/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                     CTACGCTAAGCCAGAAAGATTCCTCGGTATCGTTAAGTACCCAAACATTGAACGCGGATTCCCATTCAACCGCCACACTGACTTTTATTGCGCATGATGCAGCAGGTAATCCTGTTGTCGGGCTGGTGCTCTCGACGCGTCACGAAGGTGTTCAGGACATCACCCTTTCTGACTGGAAAGATAATGGTGACGGAAGCTATACCCAGATCCTGACCACAGGAGCGATGTCTGGCACGCTGACGCTGATGCCACAGCTGAA  >  NC_000913/2046520‑2046778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑