Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,053,628	A→G	intergenic (‑300/‑15)	yeeL ← / → shiA	putative glycosyltransferase, N‑terminal fragment/shikimate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,053,628	0	A	G	100.0%	131.8 / NA	38	intergenic (‑300/‑15)	yeeL/shiA	putative glycosyltransferase, N‑terminal fragment/shikimate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/17);  total (21/17)

TCGAATTTTTCATGTTGCCGCTAATCAGCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTACAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTAC  >  NC_000913/2053492‑2053760                                                                                                                                         |                                                                                                                                     tCGAATTTTTCATGTTGCCGCTAATCAGCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCaa                                                                                                                                   >  1:494215/1‑140 (MQ=255)            aTGTTGCCGCTAATCAGCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCAtt                                                                                                                        <  2:58802/140‑1 (MQ=255)                        aTCAGCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACg                                                                                                            >  2:728816/1‑140 (MQ=255)                        aTCAGCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACg                                                                                                            <  1:728816/140‑1 (MQ=255)                            gCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCa                                                                                                        >  2:723470/1‑140 (MQ=255)                             ccATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCAt                                                                                                       >  2:371731/1‑140 (MQ=255)                                  aaaTCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCa                                                                                                  <  2:494215/140‑1 (MQ=255)                                   aaTCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCAc                                                                                                 <  2:69835/140‑1 (MQ=255)                                           gCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCg                                                                                         <  1:723470/140‑1 (MQ=255)                                                       tGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAggg                                                                                 <  2:133773/136‑1 (MQ=255)                                                       tGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAggg                                                                                 >  1:133773/1‑136 (MQ=255)                                                            aGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCg                                                                        >  2:102984/1‑140 (MQ=255)                                                                       tAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCgcgc                                                             >  2:679301/1‑140 (MQ=255)                                                                          ggTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAATGGACGCTTTCGTTAAGTCGCGCCcg                                                          <  1:506488/140‑1 (MQ=255)                                                                          ggTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCcg                                                          <  1:679301/140‑1 (MQ=255)                                                                            ttCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCcgac                                                        >  1:465178/1‑140 (MQ=255)                                                                            ttCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCcgac                                                        >  2:541687/1‑140 (MQ=255)                                                                              cGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCcgacga                                                      >  2:105681/1‑140 (MQ=255)                                                                              cGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCcgacga                                                      >  2:359855/1‑140 (MQ=255)                                                                              cGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCAC‑CGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAg                                                     >  1:319477/1‑140 (MQ=255)                                                                               gTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAg                                                     <  1:371731/140‑1 (MQ=255)                                                                               gTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAg                                                     <  1:169998/140‑1 (MQ=255)                                                                               gTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAg                                                     <  1:102984/140‑1 (MQ=255)                                                                                           aCGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAg                                                     <  2:289844/128‑1 (MQ=255)                                                                                           aCGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAg                                                     >  1:289844/1‑128 (MQ=255)                                                                                           aCGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCa                                         >  2:582726/1‑140 (MQ=255)                                                                                           aCGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCa                                         >  2:12979/1‑140 (MQ=255)                                                                                                 aTGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCg                                   <  1:105681/140‑1 (MQ=255)                                                                                                         tACCTTTTCCATACTAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGcc                           >  2:318704/1‑140 (MQ=255)                                                                                                                       tAATTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTa             <  1:318704/140‑1 (MQ=255)                                                                                                                         aTTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGGTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATg           >  2:632673/1‑140 (MQ=255)                                                                                                                         aTTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTg                             >  1:268068/1‑122 (MQ=255)                                                                                                                         aTTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTg                             <  2:268068/122‑1 (MQ=255)                                                                                                                         aTTATAAAAAGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATg           >  2:610147/1‑140 (MQ=255)                                                                                                                              aaaaaGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGAtttt      >  2:341164/1‑140 (MQ=255)                                                                                                                              aaaaaGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGAtttt      <  2:449058/140‑1 (MQ=255)                                                                                                                              aaaaaGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGAtttt      >  2:576547/1‑140 (MQ=255)                                                                                                                                  aGACAGTGCAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTAc  <  1:12979/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TCGAATTTTTCATGTTGCCGCTAATCAGCCATAAAATCATTTGCAGATGCGCTCTGTCGAGGTAGTCTCATAAGGTTCGTTTATAGATCGACGGCAATGTGAGTTACCTTTTCCATACTAATTATAAAAAGACAGTACAAACAGGATCATTATGGACTCCACGCTCATCTCCACTCGTCCCGATGAAGGGACGCTTTCGTTAAGTCGCGCCCGACGAGCTGCGTTAGGCAGCTTCGCTGGTGCCGTCGTCGACTGGTATGATTTTTTAC  >  NC_000913/2053492‑2053760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑