Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,059,306	A→G	F181L (TTT→CTT)	yeeO ←	FMN/FAD exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,059,306	0	A	G	100.0%	183.5 / NA	52	F181L (TTT→CTT)	yeeO	FMN/FAD exporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/29);  total (23/29)

CTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGAC  >  NC_000913/2059171‑2059432                                                                                                                                        |                                                                                                                               ctgctgGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAagcaac                                                                                                                            <  2:54689/140‑1 (MQ=255)        aTAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGa                                                                                                                     <  1:203545/140‑1 (MQ=255)         tAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGAt                                                                                                                    <  1:108264/140‑1 (MQ=255)         tAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGAt                                                                                                                    >  1:358490/1‑140 (MQ=255)               aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCAcc                                                                                                                <  1:655727/138‑1 (MQ=255)               aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCAcc                                                                                                                >  2:655727/1‑138 (MQ=255)               aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCaa                                                                                                              >  1:276087/1‑140 (MQ=255)               aGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCaa                                                                                                              >  1:537406/1‑140 (MQ=255)                 tACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCAt                                                                                                                       >  2:417444/1‑129 (MQ=255)                 tACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCAt                                                                                                                       <  1:417444/129‑1 (MQ=255)                 tACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATg                                                                                                            >  1:361160/1‑140 (MQ=255)                      tCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGc                                                                                                       >  1:13099/1‑140 (MQ=255)                         gCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCcgc                                                                                                    >  1:330649/1‑140 (MQ=255)                                    gTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCct                                                                                         >  2:374704/1‑140 (MQ=255)                                     tCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCctc                                                                                        >  1:536038/1‑140 (MQ=255)                                       aaCGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCctcgc                                                                                      <  2:276087/140‑1 (MQ=255)                                                  tttAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGAt                                                                           <  2:330649/140‑1 (MQ=255)                                                      aCTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCg                                                                       <  1:403898/140‑1 (MQ=255)                                                           tGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTAc                                                                  >  2:28900/1‑140 (MQ=255)                                                           tGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTAc                                                                  >  2:166589/1‑140 (MQ=255)                                                           tGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTAc                                                                  >  1:26766/1‑140 (MQ=255)                                                                 ggCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGAc                                                            >  1:730624/1‑140 (MQ=255)                                                                      cACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAAt                                                       <  2:536038/140‑1 (MQ=255)                                                                       aCCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATg                                                      >  1:235413/1‑140 (MQ=255)                                                                       aCCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATg                                                      >  1:22004/1‑140 (MQ=255)                                                                         ccGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTGCCGAGACTAAATGcc                                                    <  2:499402/140‑1 (MQ=255)                                                                              acgaAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAAc                                               <  2:456159/140‑1 (MQ=255)                                                                              acgaAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAAc                                               <  2:428000/140‑1 (MQ=255)                                                                                gaAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGa                                             <  1:399975/140‑1 (MQ=255)                                                                                gaAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGa                                             <  2:22004/140‑1 (MQ=255)                                                                                gaAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGa                                             <  1:690830/140‑1 (MQ=255)                                                                                 aaaTCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGAc                                            >  2:598087/1‑140 (MQ=255)                                                                                  aaTCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAAGGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACa                                           >  2:449219/1‑140 (MQ=255)                                                                                   aTCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACag                                          <  2:361160/140‑1 (MQ=255)                                                                                   aTCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACag                                          <  2:358490/140‑1 (MQ=255)                                                                                     cAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACagta                                        <  2:730624/140‑1 (MQ=255)                                                                                       ataatTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACagtagt                                      >  1:246459/1‑140 (MQ=255)                                                                                        taatTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAgtagta                                     <  1:374704/140‑1 (MQ=255)                                                                                        taatTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAgtagta                                     <  1:281577/140‑1 (MQ=255)                                                                                          atTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTAcc                                   <  1:166589/140‑1 (MQ=255)                                                                                           tttGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCa                                  <  1:731518/140‑1 (MQ=255)                                                                                                tCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTAc                                    <  2:335953/133‑1 (MQ=255)                                                                                                tCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTAc                                    >  1:335953/1‑133 (MQ=255)                                                                                                tCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATc                             >  2:239673/1‑140 (MQ=255)                                                                                                  gCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACag                                          >  1:542478/1‑125 (MQ=255)                                                                                                  gCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACag                                          <  2:542478/125‑1 (MQ=255)                                                                                                                 aaGCGTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAg            <  2:246459/140‑1 (MQ=255)                                                                                                                     gTTGCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCAt        <  1:28900/140‑1 (MQ=255)                                                                                                                        gCCAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAAt     >  1:543213/1‑140 (MQ=255)                                                                                                                          cAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGa   <  1:598087/140‑1 (MQ=255)                                                                                                                          cAACAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGa   <  1:449219/140‑1 (MQ=255)                                                                                                                           aaCAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGAc  <  2:13099/140‑1 (MQ=255)                                                                                                                           aaCAGTACGGCAAGCAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGAc  <  1:104560/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               CTGCTGGATAACTGAGTACCGTCAGCTCCAGATAAGTCAACGCCAGTGCTTTAACTTCTGTCGTGGCATCACCCGCGACGAAATCAATAATTTGTTCGCCAAAATGATGAATAAGCGTTGCCAACAGTACGGCAAACAACGTCATGATCACCAATGACTGCCGCGTCGCCACCCTCGCTCGTCGTCGATCCCGCTTACCGAGACTAAATGCCACAACGACAGTAGTACCAAGATCGATAGCAGCAAAAAAAGCCATAATGAC  >  NC_000913/2059171‑2059432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑