Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,080,216	T→C	T126A (ACG→GCG)	yeeA ←	putative transporter YeeA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,080,216	0	T	C	100.0%	91.8 / NA	27	T126A (ACG→GCG)	yeeA	putative transporter YeeA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/12);  total (15/12)

AGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATC  >  NC_000913/2080084‑2080352                                                                                                                                      |                                                                                                                                         aGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCca                                                                                                                                   <  2:136419/140‑1 (MQ=255)       tGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCcaatcaat                                                                                                                             <  2:460135/140‑1 (MQ=255)          aCGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATaaa                                                                                                                          <  1:569127/140‑1 (MQ=255)            gCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCCACAACAATTGCCAGCGCCACCCCAATCAATAAAc                                                                                                                         >  1:590424/1‑140 (MQ=255)                    tgGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACg                                                                                                                <  2:498886/140‑1 (MQ=255)                                                   aaaGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAg                                                                                 >  1:303166/1‑140 (MQ=255)                                                     agagCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAACCCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAgg                                                                                <  2:590424/140‑1 (MQ=255)                                                         ccGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACAt                                                                           >  2:245597/1‑140 (MQ=255)                                                           gAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATgg                                                                         >  1:588651/1‑140 (MQ=255)                                                            aGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGgc                                                                        >  2:502348/1‑140 (MQ=255)                                                                   caTCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCcgcg                                                                 >  2:549172/1‑140 (MQ=255)                                                                   caTCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCcgcg                                                                 >  1:317455/1‑140 (MQ=255)                                                                     tCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCa                                                               <  1:502348/140‑1 (MQ=255)                                                                        ccgcTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCa                                                            >  2:282494/1‑140 (MQ=255)                                                                                      cGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCg                                              <  2:588651/140‑1 (MQ=255)                                                                                         cGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTa                                           >  2:612212/1‑140 (MQ=255)                                                                                            aTCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACg                                        <  1:282494/140‑1 (MQ=255)                                                                                             tCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACga                                       >  1:269505/1‑140 (MQ=255)                                                                                                 tttCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGAtt                                   >  1:277151/1‑140 (MQ=255)                                                                                                         gTCGGGGAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAg                           >  2:151195/1‑140 (MQ=255)                                                                                                              ggAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCa                      <  2:317455/140‑1 (MQ=255)                                                                                                               gAA‑CCCACAACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCag                     >  1:372724/1‑140 (MQ=255)                                                                                                                     cacaACAATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGa                >  1:559648/1‑140 (MQ=255)                                                                                                                      acaacaATTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGAt               <  2:559648/140‑1 (MQ=255)                                                                                                                            aTTGCCAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGAcc         <  2:277151/140‑1 (MQ=255)                                                                                                                                ccAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATa              >  2:209793/1‑131 (MQ=255)                                                                                                                                ccAGCGCCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATa              <  1:209793/131‑1 (MQ=255)                                                                                                                                   gcgcCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATc  <  1:549172/140‑1 (MQ=255)                                                                                                                                   gcgcCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATc  <  1:245597/140‑1 (MQ=255)                                                                                                                                   gcgcCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATc  <  1:151195/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         AGTGGATGAACGCCCGTTGTGGCCAGATACCGGTAAACAACATTGCCAGTAAAGAGCCGAGGATCACATCGCCGCTTCGCCATAACGCCGTATCAATTTCACCTGTCGGGGAA‑CCCACAACAATTGCCAGCGTCACCCCAATCAATAAACCTTGATACGGTTTCTTGCCCAGCGCCAGCCAACCGCAAAGGAACATGGCCGCCGCGCACCAGACTAACATCAGCGGTAACGAGATTAACTCCAGTTGCAGAGCGATAAGACCTAAAATC  >  NC_000913/2080084‑2080352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑