| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,081,846 | A→C | G234G (GGT→GGG) | dacD ← | D‑alanyl‑D‑alanine carboxypeptidase DacD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,081,846 | 0 | A | C | 96.4% | 88.0 / ‑4.0 | 28 | G234G (GGT→GGG) | dacD | D‑alanyl‑D‑alanine carboxypeptidase DacD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (13/14); minor base T (0/1); total (13/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.09e-01 | |||||||||||
ACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTG > NC_000913/2081747‑2081971 | aCGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCcaca > 2:385207/1‑140 (MQ=255) gACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCacaac > 2:226787/1‑140 (MQ=255) ccTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCAcaacaa > 2:724767/1‑140 (MQ=255) cATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTggg < 1:724767/140‑1 (MQ=255) ttACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTg > 2:283534/1‑140 (MQ=255) aCCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGAt < 1:42812/140‑1 (MQ=255) tGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGt > 2:388343/1‑140 (MQ=255) cAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTc > 2:95948/1‑140 (MQ=255) cgacgCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTgag > 2:594749/1‑140 (MQ=255) acgCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGAc < 1:594749/140‑1 (MQ=255) cTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACttt > 2:80786/1‑140 (MQ=255) cccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct < 1:388343/140‑1 (MQ=255) ccATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtctc < 1:385207/140‑1 (MQ=255) cATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCa < 1:284030/140‑1 (MQ=255) aaGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct < 1:199710/126‑1 (MQ=255) aaGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct > 2:199710/1‑126 (MQ=255) aaGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGa > 2:311369/1‑140 (MQ=255) aGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGTTGATACCGTTCCAGGTGAGACTTTtct > 2:672370/1‑125 (MQ=255) aGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct < 2:114968/125‑1 (MQ=255) aGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct < 1:672370/125‑1 (MQ=255) aGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct > 1:634234/1‑125 (MQ=255) aGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct < 2:634234/125‑1 (MQ=255) aGCAATGAGATTAAACCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTtct > 1:114968/1‑125 (MQ=255) aaaCCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCt < 1:56791/140‑1 (MQ=255) aaaCCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCt < 1:311369/140‑1 (MQ=255) aCCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCg < 1:89605/140‑1 (MQ=255) aCCCGGCACCAGAAGTATGCCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCg < 1:226787/140‑1 (MQ=255) gCACCAGAAGTATGTCCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTg < 2:341938/140‑1 (MQ=255) | ACGACCTTTTGCACTGTCAGCCCCCATTACCACTGCAATGAGACGACGCTGCCCATCTACAGCCGAAGCAATGAGATTAAACCCGGCACCAGAAGTATGACCCGTTTTCAGGCCGTCAACATTCATGGTTTTATCCCACAACAACCCGTTACGGTTTTGCTGGGTGATACCGTTCCAGGTGAGACTTTTCTCACTGTACATATGATAAAACTCGGGCTCGCCGTG > NC_000913/2081747‑2081971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |