Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	145,712	A→G	Y211C (TAC→TGC)	yadE →	putative polysaccharide deacetylase lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	145,712	0	A	G	97.1%	232.8 / ‑2.4	69	Y211C (TAC→TGC)	yadE	putative polysaccharide deacetylase lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (29/38);  total (30/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

ACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTATCCTGTGTTGAAACAATA  >  NC_000913/145575‑145847                                                                                                                                          |                                                                                                                                        aCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGcg                                                                                                                                       >  2:206840/1‑140 (MQ=255) aCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGcg                                                                                                                                       <  2:142735/140‑1 (MQ=255) aCCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGcg                                                                                                                                       >  1:528309/1‑140 (MQ=255)      aTAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGaca                                                                                                                                  <  2:603779/140‑1 (MQ=255)      aTAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGaca                                                                                                                                  <  1:224779/140‑1 (MQ=255)        aaTGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACt                                                                                                                                <  2:30211/140‑1 (MQ=255)                 tCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGt                                                                                                                       >  2:362024/1‑140 (MQ=255)                   ggTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGc                                                                                                                     >  2:22611/1‑140 (MQ=255)                          aCCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGa                                                                                                              <  1:362024/140‑1 (MQ=255)                               tCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCt                                                                                                         >  1:326411/1‑140 (MQ=255)                               tCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCt                                                                                                         >  1:662253/1‑140 (MQ=255)                               tCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCt                                                                                                         <  1:411268/140‑1 (MQ=255)                                    atatTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                                                                                    <  1:22611/140‑1 (MQ=255)                                      atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaa                                                                                                  >  2:641191/1‑140 (MQ=255)                                      atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                                                                                    >  2:93899/1‑138 (MQ=255)                                      atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                                                                                    <  1:93899/138‑1 (MQ=255)                                      atTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaa                                                                                                  >  2:408782/1‑140 (MQ=255)                                          tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAAt                                                                                              <  1:447977/140‑1 (MQ=255)                                          tGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAAt                                                                                              >  1:301576/1‑140 (MQ=255)                                           gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATa                                                                                             <  1:638155/140‑1 (MQ=255)                                           gcgcGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATa                                                                                             >  2:418430/1‑140 (MQ=255)                                            cgcgACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATaa                                                                                            >  1:677633/1‑140 (MQ=255)                                             gcgACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAg                                                                                           >  1:86370/1‑140 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaagaa                                                                                               <  1:30233/132‑1 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaagaa                                                                                               <  2:659181/132‑1 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaagaa                                                                                               >  2:30233/1‑132 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTgaagaa                                                                                               >  1:659181/1‑132 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                                                                                    <  2:411231/127‑1 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTg                                                                                                    >  1:411231/1‑127 (MQ=255)                                                 cgaAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCa                                                                                       >  2:383374/1‑140 (MQ=255)                                                       aaaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTcc                                                                                 <  2:586943/140‑1 (MQ=255)                                                        aaCACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTccc                                                                                <  1:383374/140‑1 (MQ=255)                                                                ttttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg                                                                        >  2:624726/1‑140 (MQ=255)                                                                ttttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg                                                                        >  2:344574/1‑140 (MQ=255)                                                                ttttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg                                                                        <  2:202724/140‑1 (MQ=255)                                                                ttttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg                                                                        <  1:624726/140‑1 (MQ=255)                                                                ttttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAg                                                                        <  2:79192/140‑1 (MQ=255)                                                                  ttCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCg                                                                      <  1:206840/140‑1 (MQ=255)                                                                   tCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCgg                                                                     <  2:115959/140‑1 (MQ=255)                                                                   tCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCgg                                                                     <  1:641191/140‑1 (MQ=255)                                                                          aCTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGAt                                                              <  1:252980/140‑1 (MQ=255)                                                                                    ccACATCGGCACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTtgat                                                    >  2:21047/1‑140 (MQ=255)                                                                                      acaTCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTtgatga                                                  <  2:301576/140‑1 (MQ=255)                                                                                            gTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCt                                            <  2:412120/140‑1 (MQ=255)                                                                                             tACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTtgatg                                                   >  1:158011/1‑132 (MQ=255)                                                                                             tACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTtgatg                                                   <  2:158011/132‑1 (MQ=255)                                                                                             tACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTc                                           >  1:665491/1‑140 (MQ=255)                                                                                                 cgcTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGt                                       >  1:153075/1‑140 (MQ=255)                                                                                                  gcTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTAAAGTc                                      >  2:523709/1‑140 (MQ=255)                                                                                                       cAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGa                                 <  1:418430/140‑1 (MQ=255)                                                                                                       cAATAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGa                                 <  1:487897/140‑1 (MQ=255)                                                                                                        aaTAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAg                                <  2:89596/140‑1 (MQ=255)                                                                                                        aaTAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAg                                <  2:153075/140‑1 (MQ=255)                                                                                                        aaTAACCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAg                                <  2:665491/140‑1 (MQ=255)                                                                                                            aCCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGc                            <  1:344574/140‑1 (MQ=255)                                                                                                            aCCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGc                            <  2:677633/140‑1 (MQ=255)                                                                                                            aCCAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGc                            <  2:326411/140‑1 (MQ=255)                                                                                                              cAGATGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTa                          >  1:20638/1‑140 (MQ=255)                                                                                                              cAGACGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGc                            >  1:574235/1‑138 (MQ=255)                                                                                                              cAGACGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGc                            <  2:574235/138‑1 (MQ=255)                                                                                                                 aTGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGc                       <  1:408782/140‑1 (MQ=255)                                                                                                                  tGGCCTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCg                      >  2:726415/1‑140 (MQ=255)                                                                                                                     ccTGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTAt                   >  2:136816/1‑140 (MQ=255)                                                                                                                       tGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGc                       >  1:680546/1‑134 (MQ=255)                                                                                                                       tGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGc                       <  2:680546/134‑1 (MQ=255)                                                                                                                       tGGCTGCGTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTATcc                 <  2:86370/140‑1 (MQ=255)                                                                                                                              gTGACAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTATCCTGTGTTg          >  2:373791/1‑140 (MQ=255)                                                                                                                                  cAGGGGATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTATCCTGTGTTGAAAc      <  1:716657/140‑1 (MQ=255)                                                                                                                                      ggATGCGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTATCCTGTGTTGAAACAata  <  2:20638/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        ACCCGATAATGGCCTGTCGGTGCTAACCTATCACCATATTCTGCGCGACGAAGAAAACACCCGTTTTCGCCATACTTCGACGACCACATCGGTACGCGCTTTCAATAACCAGATGGCCTGGCTGCGTGACAGGGGATACGCGACACTGAGCATGGTGCAGCTGGAAGGCTACGTGAAGAATAAGATCAATCTCCCTGCGCGAGCGGTGGTGATTACCTTTGATGATGGCCTCAAGTCGGTGAGCCGCTATGCGTATCCTGTGTTGAAACAATA  >  NC_000913/145575‑145847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑