Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,108,622	C→A	G321G (GGG→GGT)	rfbX ←	polyisoprenol‑linked O‑antigen repeat unit flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,108,622	0	C	A	100.0%	172.1 / NA	49	G321G (GGG→GGT)	rfbX	polyisoprenol‑linked O‑antigen repeat unit flippase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/17);  total (32/17)

TACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTAC  >  NC_000913/2108498‑2108755                                                                                                                             |                                                                                                                                      tACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTAttttt                                                                                                                        <  2:399727/140‑1 (MQ=255)      aaTTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAg                                                                                                                   >  2:246693/1‑140 (MQ=255)              cttATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATg                                                                                                           <  2:114135/140‑1 (MQ=255)                             cAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAagag                                                                                                 <  2:97053/135‑1 (MQ=255)                             cAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAagag                                                                                                 >  1:97053/1‑135 (MQ=255)                                         aaTGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAg                                                                                <  2:40955/140‑1 (MQ=255)                                         aaTGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTAGACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAg                                                                                >  2:652479/1‑140 (MQ=255)                                          aTGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGc                                                                               >  1:1183/1‑140 (MQ=255)                                             ccATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGcacc                                                                            <  1:652479/140‑1 (MQ=255)                                                      ttACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCaa                                                                   <  1:246693/140‑1 (MQ=255)                                                       tACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCaaa                                                                  >  2:354619/1‑140 (MQ=255)                                                       tACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCaaa                                                                  >  1:213574/1‑140 (MQ=255)                                                       tACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCaaa                                                                  >  2:395004/1‑140 (MQ=255)                                                            aaTGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAAt                                                                      >  1:404146/1‑131 (MQ=255)                                                            aaTGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAAt                                                                      <  2:404146/131‑1 (MQ=255)                                                            aaTGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCaa                                                             >  2:94594/1‑140 (MQ=255)                                                                   tAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTc                                                      >  1:499924/1‑140 (MQ=255)                                                                          aGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGAtt                                               >  2:229985/1‑140 (MQ=255)                                                                                agGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTttaa                                         >  2:362256/1‑140 (MQ=255)                                                                                 ggCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCGATCAAACTCAAGCAGGTCAATGATTTAttaat                                        >  2:644510/1‑140 (MQ=255)                                                                                 ggCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTttaat                                        >  1:382332/1‑140 (MQ=255)                                                                                 ggCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTttaat                                        >  2:29406/1‑140 (MQ=255)                                                                                     atatAATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTaaa                                    >  1:200266/1‑140 (MQ=255)                                                                                      tataATCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTaaaa                                   >  2:707888/1‑140 (MQ=255)                                                                                          aTCATTAGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATcac                               <  1:29406/140‑1 (MQ=255)                                                                                                aGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATca                                <  1:632541/133‑1 (MQ=255)                                                                                                aGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATca                                >  2:632541/1‑133 (MQ=255)                                                                                                aGCACAATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCtt                         >  1:31294/1‑140 (MQ=255)                                                                                                  cacaATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTca                       >  2:182779/1‑140 (MQ=255)                                                                                                  cacaATCACTGCATTATCATATCCCGGACCTATACATATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTca                       <  2:509469/140‑1 (MQ=255)                                                                                                    caATCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTcaca                     <  1:229985/140‑1 (MQ=255)                                                                                                     aaTCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTcac                      <  1:511157/138‑1 (MQ=255)                                                                                                     aaTCACTGCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTcac                      >  2:511157/1‑138 (MQ=255)                                                                                                            gCATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAg             >  2:677452/1‑140 (MQ=255)                                                                                                             cATTATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGa            >  1:50992/1‑140 (MQ=255)                                                                                                               ttATCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAAc          >  1:209014/1‑140 (MQ=255)                                                                                                                 aTCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACaa        <  1:182779/140‑1 (MQ=255)                                                                                                                 aTCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACaa        >  1:494272/1‑140 (MQ=255)                                                                                                                  tCATATCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAg       >  1:376904/1‑140 (MQ=255)                                                                                                                  tCATATCCCAGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAg       >  1:479808/1‑140 (MQ=255)                                                                                                                    atatCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCg     <  1:362256/140‑1 (MQ=255)                                                                                                                     tatCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTcaca                     >  1:298394/1‑123 (MQ=255)                                                                                                                     tatCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTcaca                     <  2:298394/123‑1 (MQ=255)                                                                                                                     tatCCCAGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGt    <  2:479808/140‑1 (MQ=255)                                                                                                                      atCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTa   <  2:31294/140‑1 (MQ=255)                                                                                                                       tCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTAc  >  1:40733/1‑140 (MQ=255)                                                                                                                       tCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTAc  >  2:63308/1‑140 (MQ=255)                                                                                                                       tCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTAc  >  2:303525/1‑140 (MQ=255)                                                                                                                       tCCCGGACCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTAc  >  2:300218/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                      TACTGAATTCTTTCTTATAATTATGGGTCAGCATAACTTGAATGCCATAGACATTACTTAATGAAATAAGAAAAGGCAGAGGCGATATAATCATTAGCACAATCACTGCATTATCATATCCCGGCCCTATACTTATTTTTACTAGTATAGATGCACCCAAGAGCAGAATTAATGAAAAAGCACCACCAATCAAACTCAAGCAGGTCAATGATTTTTTAATTAAAATCACACCCTTCACACGATTAAGAACAAGCGTAC  >  NC_000913/2108498‑2108755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑