| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,316,188 | G→A | M4I (ATG→ATA) | rcsB → | DNA‑binding transcriptional activator RcsB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,316,188 | 0 | G | A | 97.6% | 146.8 / ‑5.2 | 42 | M4I (ATG→ATA) | rcsB | DNA‑binding transcriptional activator RcsB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/19); minor base C (0/1); total (22/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATG > NC_000913/2316071‑2316324 | aCGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGAcc < 2:502629/140‑1 (MQ=255) cGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCa > 2:461463/1‑140 (MQ=255) tGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGAt > 1:337786/1‑132 (MQ=255) tGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGAt < 2:337786/132‑1 (MQ=255) tGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGt < 2:720935/140‑1 (MQ=255) agaAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTAt > 1:205142/1‑140 (MQ=255) gaAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGAAAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATCAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTAtt < 2:681230/140‑1 (MQ=255) aGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCg < 2:283517/140‑1 (MQ=255) aTGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCaa < 2:557263/140‑1 (MQ=255) aTGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCaa < 2:275346/140‑1 (MQ=255) ggAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTg < 1:431380/140‑1 (MQ=255) aTACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACt < 2:574285/127‑1 (MQ=255) aTACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACt > 1:574285/1‑127 (MQ=255) tACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCATATTGAGt > 2:490445/1‑140 (MQ=255) aTGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAAtt > 1:394882/1‑140 (MQ=255) tGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAAttt > 2:243131/1‑140 (MQ=255) agagCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa < 2:668648/140‑1 (MQ=255) agagCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa < 1:461463/140‑1 (MQ=255) agagCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa < 2:205142/140‑1 (MQ=255) agagCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa > 2:451118/1‑140 (MQ=255) agagCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGa < 2:394882/140‑1 (MQ=255) gCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGActc < 1:243131/140‑1 (MQ=255) tGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTAc > 2:274157/1‑140 (MQ=255) tGCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTAc > 1:575745/1‑140 (MQ=255) gCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACa > 2:162363/1‑140 (MQ=255) gCTGTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACa > 1:501552/1‑140 (MQ=255) gTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCATATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCa < 1:490445/140‑1 (MQ=255) gTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCa < 1:274157/140‑1 (MQ=255) gTAGCAAGGTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCa < 1:154098/140‑1 (MQ=255) gTAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATcaa > 1:160815/1‑140 (MQ=255) tAGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATcaac > 2:199472/1‑140 (MQ=255) aGCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATcaaca < 2:187822/140‑1 (MQ=255) gCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATcaacaa > 2:342671/1‑140 (MQ=255) gCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATcaacaa > 2:522431/1‑140 (MQ=255) gCCTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATcaacaa > 2:373921/1‑140 (MQ=255) ccTATTACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCaacaac > 2:513921/1‑140 (MQ=255) tACATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGcc > 1:205809/1‑140 (MQ=255) aCATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCg > 2:516886/1‑140 (MQ=255) aCATGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCg > 2:652361/1‑140 (MQ=255) tGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGaaa < 1:652361/140‑1 (MQ=255) tGAACAATATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGaaa > 2:367704/1‑140 (MQ=255) aaTATAAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGa < 1:516886/140‑1 (MQ=255) tataAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATg < 1:367704/140‑1 (MQ=255) | ACGCTGGAACATCTGATTCGTGAGAAGGATGTTCCAGGAATAGAAAAATACATCAGCGACATTGACAGTTATGTCAAGAGCTTGCTGTAGCAAGGTAGCCTATTACATGAACAATATGAACGTAATTATTGCCGATGACCATCCGATAGTCTTGTTCGGTATTCGCAAATCACTTGAGCAAATTGAGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATG > NC_000913/2316071‑2316324 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |