Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,372,711	T→C	V52A (GTG→GCG)	arnE →	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,372,711	0	T	C	100.0%	222.2 / NA	64	V52A (GTG→GCG)	arnE	undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnE subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (28/36);  total (28/36)

GTCTTTGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTG  >  NC_000913/2372575‑2372847                                                                                                                                         |                                                                                                                                         gTCTTTGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGct                                                                                                                                       >  2:706489/1‑140 (MQ=255)      tGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGgc                                                                                                                                  >  1:439966/1‑140 (MQ=255)      tGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGgc                                                                                                                                  >  1:565968/1‑140 (MQ=255)       gCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCGTGGCGCTCTGgct                                                                                                                                 >  2:265627/1‑140 (MQ=255)       gCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGgct                                                                                                                                 >  2:636085/1‑140 (MQ=255)       gCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGgct                                                                                                                                 >  1:89776/1‑140 (MQ=255)       gCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGgct                                                                                                                                 >  1:322722/1‑140 (MQ=255)            cttgcttAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTgg                                                                                                                            <  2:316356/140‑1 (MQ=255)            cttgcttAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTgg                                                                                                                            <  1:688215/140‑1 (MQ=255)              tgcttAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTc                                                                                                                          <  1:636085/140‑1 (MQ=255)                cttAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCtt                                                                                                                        >  1:655078/1‑140 (MQ=255)                cttAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCtt                                                                                                                        >  2:691927/1‑140 (MQ=255)                cttAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCtt                                                                                                                        >  2:313764/1‑140 (MQ=255)                   aGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCa                                                                                                                     <  1:398345/140‑1 (MQ=255)                      gTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGCTAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCCTGGCGCTCTGGCTGCTGGTCTTGCacca                                                                                                                  >  2:500357/1‑137 (MQ=255)                       ttGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAAc                                                                                                                 <  1:684051/140‑1 (MQ=255)                        tGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACg                                                                                                                <  1:691927/140‑1 (MQ=255)                         gCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGt                                                                                                               >  1:546705/1‑140 (MQ=255)                         gCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGt                                                                                                               >  1:209508/1‑140 (MQ=255)                             ggCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCg                                                                                                           <  1:591951/140‑1 (MQ=255)                               cAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGt                                                                                                         >  1:478156/1‑140 (MQ=255)                                    gtgtCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTAcc                                                                                                            >  1:555278/1‑132 (MQ=255)                                    gtgtCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTAcc                                                                                                            <  2:555278/132‑1 (MQ=255)                                    gtgtCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCa                                                                                                    <  2:707227/140‑1 (MQ=255)                                            aaCAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTAc                                                                                            >  2:587491/1‑140 (MQ=255)                                              cAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTAccc                                                                                          <  2:512133/140‑1 (MQ=255)                                              cAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTAccc                                                                                          <  1:587491/140‑1 (MQ=255)                                                     ccTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTa                                                                                   >  2:293365/1‑140 (MQ=255)                                                         cTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTc                                                                               <  1:706489/140‑1 (MQ=255)                                                         cTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTc                                                                               >  1:233410/1‑140 (MQ=255)                                                              tgGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAAt                                                                          <  1:159372/140‑1 (MQ=255)                                                               ggCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAAtt                                                                         >  2:693868/1‑140 (MQ=255)                                                               ggCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAAtt                                                                         >  2:255396/1‑140 (MQ=255)                                                               ggCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAAtt                                                                         >  2:307542/1‑140 (MQ=255)                                                                   aTAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGt                                                                     <  2:655078/140‑1 (MQ=255)                                                                     aaacaaacGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCt                                                                   <  2:439966/140‑1 (MQ=255)                                                                            cGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGAc                                                            >  1:395268/1‑140 (MQ=255)                                                                             ggCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACg                                                           <  1:24042/140‑1 (MQ=255)                                                                               cgcAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgct                                                         >  2:248906/1‑140 (MQ=255)                                                                               cgcAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgct                                                         >  1:37856/1‑140 (MQ=255)                                                                                  aaaCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggc                                                      <  2:89776/140‑1 (MQ=255)                                                                                  aaaCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggc                                                      <  1:265754/140‑1 (MQ=255)                                                                                    aCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggctg                                                    <  1:693868/140‑1 (MQ=255)                                                                                    aCATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACgctggctg                                                    <  2:478156/140‑1 (MQ=255)                                                                                     cATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGc                                                   <  2:322722/140‑1 (MQ=255)                                                                                              cTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACt                                          >  1:282424/1‑140 (MQ=255)                                                                                               tgtgGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACtg                                         <  2:701899/140‑1 (MQ=255)                                                                                                   gCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGTAGAACGTACCGGTAAGCATTGCTTACCCGATGTTAAGACTGAATTTTGGCTGGGTGCCGCTCGCTGCACTAAAACTGTGGc                                     >  2:535471/1‑140 (MQ=255)                                                                                                   gCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGc                                     >  2:158567/1‑140 (MQ=255)                                                                                                    cTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCa                                    <  1:486562/140‑1 (MQ=255)                                                                                                    cTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCa                                    <  2:565968/140‑1 (MQ=255)                                                                                                         aCTGGCGCTGGCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAAc                               <  2:546705/140‑1 (MQ=255)                                                                                                             gcgcTGGCTTGTCTTGGTCTTGCCATGGCGCTATGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGt                           >  2:419842/1‑140 (MQ=255)                                                                                                                  ggCTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgc                      <  2:282424/140‑1 (MQ=255)                                                                                                                    cTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgccg                    <  1:255396/140‑1 (MQ=255)                                                                                                                    cTTGTCTTGGTCTTGCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATcgccg                    <  1:172632/140‑1 (MQ=255)                                                                                                                       gtcttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGt                 <  1:158567/140‑1 (MQ=255)                                                                                                                        tcttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTc                >  1:359714/1‑140 (MQ=255)                                                                                                                         cttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGTAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa               <  1:535471/140‑1 (MQ=255)                                                                                                                         cttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa               <  1:313764/140‑1 (MQ=255)                                                                                                                         cttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa               <  2:233410/140‑1 (MQ=255)                                                                                                                         cttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa               <  1:248906/140‑1 (MQ=255)                                                                                                                         cttggtcttgCCATGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCa               <  2:395268/140‑1 (MQ=255)                                                                                                                                     aTGGCGCTATGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGt   <  1:419842/140‑1 (MQ=255)                                                                                                                                      tGGCGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTg  <  2:209508/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GTCTTTGCCAGCTTGCTTAGCGTTGCCGGGCAGTTGTGTCAGAAACAGGCAACCTGCTTTGTGGCGATAAACAAACGGCGCAAACATATCGTGCTGTGGCTGGGACTGGCGCTGGCTTGTCTTGGTCTTGCCATGGTGCTCTGGCTGCTGGTCTTGCAGAACGTACCGGTAGGCATTGCTTACCCGATGTTAAGTCTGAATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTG  >  NC_000913/2372575‑2372847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑