Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,435,973	T→C	D226G (GAC→GGC)	usg ←	putative semialdehyde dehydrogenase Usg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,435,973	0	T	C	100.0%	164.2 / NA	47	D226G (GAC→GGC)	usg	putative semialdehyde dehydrogenase Usg
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/24);  total (23/24)

ATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCT  >  NC_000913/2435840‑2436089                                                                                                                                      |                                                                                                                     atCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCa                                                                                                                <  2:314705/140‑1 (MQ=255)   cTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAgg                                                                                                              <  2:526935/140‑1 (MQ=255)   cTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAgg                                                                                                              <  1:730484/140‑1 (MQ=255)   cTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAgg                                                                                                              <  1:452981/140‑1 (MQ=255)     tCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGAt                                                                                                            >  2:626678/1‑140 (MQ=255)     tCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGAt                                                                                                            >  1:551538/1‑140 (MQ=255)          ttGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAgg                                                                                                              >  1:647791/1‑133 (MQ=255)          ttGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAgg                                                                                                              <  2:647791/133‑1 (MQ=255)           tGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCg                                                                                                      <  1:383002/140‑1 (MQ=255)           tGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCg                                                                                                      <  2:107006/140‑1 (MQ=255)           tGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCg                                                                                                      <  2:636135/140‑1 (MQ=255)            gAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGt                                                                                                     <  2:551538/140‑1 (MQ=255)            gAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGt                                                                                                     >  2:417654/1‑140 (MQ=255)            gAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGt                                                                                                     >  1:482005/1‑140 (MQ=255)                  aCGCATCACGCGCTTCTTCTGCCGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCg                                                                                               >  1:445761/1‑140 (MQ=255)                      aTCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCaa                                                                                           <  1:627922/140‑1 (MQ=255)                       tCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAAc                                                                                          >  1:503430/1‑140 (MQ=255)                       tCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAAc                                                                                          >  2:234514/1‑140 (MQ=255)                          cgcgCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGAt                                                                                       >  2:411217/1‑140 (MQ=255)                          cgcgCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGAt                                                                                       >  1:302596/1‑140 (MQ=255)                                      gctgcCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGGCGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGttcttc                                                                           <  2:457236/140‑1 (MQ=255)                                         gcCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACTGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGCCAACGATACGACGTTCTTcacg                                                                        >  1:477666/1‑140 (MQ=255)                                           cAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTcacgca                                                                      >  1:456701/1‑140 (MQ=255)                                            aGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTcacgcac                                                                     >  2:246108/1‑140 (MQ=255)                                            aGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTcacgcac                                                                     <  1:103898/140‑1 (MQ=255)                                            aGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTcacgcac                                                                     >  2:103898/1‑140 (MQ=255)                                              tGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGttc                                                                              <  1:350630/129‑1 (MQ=255)                                              tGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGttc                                                                              >  2:350630/1‑129 (MQ=255)                                                        gCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCt                                                         >  1:677372/1‑140 (MQ=255)                                                             aaaGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCg                                                    <  2:302596/140‑1 (MQ=255)                                                             aaaGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCg                                                    <  2:503430/140‑1 (MQ=255)                                                                gTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCa                                                 <  1:417654/140‑1 (MQ=255)                                                                    aCCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTCCCTTCGCTATCCGGCAGTaa                                             <  2:482005/140‑1 (MQ=255)                                                                    aCCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTaa                                             >  1:42476/1‑140 (MQ=255)                                                                      cATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACg                                           <  1:246108/140‑1 (MQ=255)                                                                       aTCTGGGCATGACTGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGCCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACgg                                          <  2:477666/140‑1 (MQ=255)                                                                         cTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGgca                                        <  2:456701/140‑1 (MQ=255)                                                                                   ccGTAGAATACCGGTACCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGaa                              >  1:390294/1‑140 (MQ=255)                                                                                           tACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCt                      >  2:668591/1‑140 (MQ=255)                                                                                                   ccTGGACGACGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCg              >  2:502339/1‑140 (MQ=255)                                                                                                       gacgacGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCgaaga          <  1:234514/140‑1 (MQ=255)                                                                                                       gacgacGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCgaaga          >  2:187430/1‑140 (MQ=255)                                                                                                       gacgacGCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCgaaga          >  1:646080/1‑140 (MQ=255)                                                                                                           acgCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGcc                >  1:650078/1‑130 (MQ=255)                                                                                                           acgCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGcc                <  2:650078/130‑1 (MQ=255)                                                                                                            cgCTAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAAtct     <  1:593225/140‑1 (MQ=255)                                                                                                               tAGCCGAAATCATCAGCCCTTCGCCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAAtcttct  <  2:677372/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                     ATCTTCGCCTTGAACAAACGCATCACGCGCTTCTTCTGCTGCCAGTGGACGCAGAGCTTCAAAGTTGACCATCTGGGCATGACCGTAGAATACCGGTGCCTGGACGACGCTAGCCGAAATCATCAGCCCTTCGTCCTGCAGGATTTTGCGTACTTCGTCAACGATACGACGTTCTTCACGCACGCTACCTTCGCTATCCGGCAGTAACGGCAGCATGTTGAACGCCAGCTGACGCCCGAAGAAATCTTCT  >  NC_000913/2435840‑2436089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑