Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,490,474	G→A	P493S (CCA→TCA)	oxc ←	oxalyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,490,474	0	G	A	100.0%	160.0 / NA	45	P493S (CCA→TCA)	oxc	oxalyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/18);  total (27/18)

ATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCA  >  NC_000913/2490338‑2490607                                                                                                                                         |                                                                                                                                      ataatGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTtgatga                                                                                                                                    <  1:725528/140‑1 (MQ=255) ataatGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTtgatga                                                                                                                                    <  1:395872/140‑1 (MQ=255)    atGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCGTGCATGGTGCAACAGATCCGTTGATGATgg                                                                                                                                 >  2:385396/1‑140 (MQ=255)       gTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGc                                                                                                                              >  1:326890/1‑140 (MQ=255)         cGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCAc                                                                                                                            >  1:90389/1‑140 (MQ=255)          ggTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCAcc                                                                                                                           >  1:215269/1‑140 (MQ=255)          ggTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCCGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCAcc                                                                                                                           >  1:380014/1‑140 (MQ=255)              tGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAgcg                                                                                                                       <  1:105889/140‑1 (MQ=255)              tGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAgcg                                                                                                                       <  1:594410/140‑1 (MQ=255)                   gACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATGCCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACGAGGGCCACt                                                                                                                  <  2:266413/140‑1 (MQ=255)                   gACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACt                                                                                                                  <  1:405028/140‑1 (MQ=255)                               gtggtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACAcc                                                                                                      >  2:45581/1‑140 (MQ=255)                                  gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACAcc                                                                                                      <  2:188862/137‑1 (MQ=255)                                  gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACAcc                                                                                                      >  1:188862/1‑137 (MQ=255)                                  gtTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGtc                                                                                                   >  1:101911/1‑140 (MQ=255)                                   ttAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGtct                                                                                                  <  1:349997/140‑1 (MQ=255)                                            gACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAg                                                                                         >  1:406836/1‑140 (MQ=255)                                                      aTCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCat                                                                               >  2:672596/1‑140 (MQ=255)                                                                gtgACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAAt                                                                     >  1:625949/1‑140 (MQ=255)                                                                    cGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACg                                                                 <  2:101911/140‑1 (MQ=255)                                                                              aaCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                       <  2:380014/140‑1 (MQ=255)                                                                              aaCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                       <  2:625949/140‑1 (MQ=255)                                                                              aaCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                       <  2:215269/140‑1 (MQ=255)                                                                              aaCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                       <  1:70603/140‑1 (MQ=255)                                                                              aaCGCCACGAAACGCATCCATTAATTTGTCATACCGTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCg                                                       <  1:385396/140‑1 (MQ=255)                                                                               aCGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCgg                                                      >  2:579243/1‑140 (MQ=255)                                                                                   cACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAgg                                                  <  1:672596/140‑1 (MQ=255)                                                                                             aTCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGAc                                        >  1:308434/1‑140 (MQ=255)                                                                                                       ttGTCATACCTTGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTc                              >  1:261689/1‑140 (MQ=255)                                                                                                                 ttGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATc                    >  2:333516/1‑140 (MQ=255)                                                                                                                 ttGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATc                    <  1:27067/140‑1 (MQ=255)                                                                                                                  tGCATGGTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATcc                   >  2:153497/1‑140 (MQ=255)                                                                                                                       ggTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg              >  1:474823/1‑140 (MQ=255)                                                                                                                       ggTGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTg              <  1:399491/140‑1 (MQ=255)                                                                                                                         tGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa            >  2:403300/1‑140 (MQ=255)                                                                                                                         tGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa            >  1:682916/1‑140 (MQ=255)                                                                                                                         tGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa            >  2:284092/1‑140 (MQ=255)                                                                                                                         tGCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaa            >  2:107197/1‑140 (MQ=255)                                                                                                                          gCAACAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCGACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGaaa           >  2:601953/1‑140 (MQ=255)                                                                                                                             aCAGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCa        <  2:682916/140‑1 (MQ=255)                                                                                                                               aGATCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCaaa      <  2:326890/140‑1 (MQ=255)                                                                                                                                 aTCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAg    >  1:512686/1‑140 (MQ=255)                                                                                                                                  tCCGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGc   >  1:36380/1‑140 (MQ=255)                                                                                                                                   ccGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCa        <  2:195620/134‑1 (MQ=255)                                                                                                                                   ccGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCa        >  1:195620/1‑134 (MQ=255)                                                                                                                                   ccGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCa  >  2:254943/1‑140 (MQ=255)                                                                                                                                   ccGTTGATGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCa  >  1:350082/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      ATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCATGGTGCAACAGATCCGTTGGTGATGGTGCACCAGCGCCACTGAGATCAACACCGTCTCCTCTGTAGATGCCGCCATTATTAAAAATAACGATCGTCACCGGCAGGTTATATCGACAAATCGTTTCAATTTCCATCCCACTGAAACCAAAAGCA  >  NC_000913/2490338‑2490607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑