Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,681,919	T→C	H276R (CAC→CGC)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,681,919	0	T	C	100.0%	182.1 / NA	52	H276R (CAC→CGC)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

GGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTT  >  NC_000913/2681798‑2682051                                                                                                                          |                                                                                                                                     ggTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCtt                                                                                                                    <  2:164455/140‑1 (MQ=255) ggTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCtt                                                                                                                    >  1:164455/1‑140 (MQ=255) ggTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCtt                                                                                                                    >  1:278256/1‑140 (MQ=255) ggTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCtt                                                                                                                    <  2:278256/140‑1 (MQ=255)    catcaGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCAt                                                                                                                 <  2:252090/140‑1 (MQ=255)     atcaGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCtt                                                                                                                    >  2:699049/1‑136 (MQ=255)     atcaGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCtt                                                                                                                    <  1:699049/136‑1 (MQ=255)      tcaGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTcctgt                                                                                                               <  1:380627/140‑5 (MQ=255)                 taCGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCAcgc                                                                                                    >  1:689106/1‑140 (MQ=255)                    gggCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCa                                                                                                                  <  1:32414/123‑1 (MQ=255)                    gggCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCa                                                                                                                  >  2:32414/1‑123 (MQ=255)                    gggCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGcc                                                                                                 >  2:453082/1‑140 (MQ=255)                    gggCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGcc                                                                                                 >  2:539512/1‑140 (MQ=255)                        cGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACa                                                                                             >  1:448330/1‑140 (MQ=255)                         gTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACaa                                                                                            >  1:97330/1‑140 (MQ=255)                                tCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa                                                                                     >  1:530967/1‑140 (MQ=255)                                tCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTa                                                                                     >  2:63853/1‑140 (MQ=255)                                       gACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAt                                                                              >  1:90145/1‑140 (MQ=255)                                           ctTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGAttttt                                                                          <  1:719157/140‑1 (MQ=255)                                            ttATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTc                                                                         <  1:396983/140‑1 (MQ=255)                                              aTCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCgg                                                                       <  2:97330/140‑1 (MQ=255)                                                cccACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGcc                                                                     <  2:689106/140‑1 (MQ=255)                                                    cGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatat                                                                 <  2:56462/140‑1 (MQ=255)                                                    cGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatat                                                                 >  2:62177/1‑140 (MQ=255)                                                    cGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatat                                                                 >  2:158916/1‑140 (MQ=255)                                                      ccTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatatat                                                               <  2:448330/140‑1 (MQ=255)                                                      ccTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatatat                                                               >  2:96804/1‑140 (MQ=255)                                                          gCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAgg                                                           >  2:385246/1‑140 (MQ=255)                                                          gCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAgg                                                           >  1:30590/1‑140 (MQ=255)                                                                ttCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGaa                                                     <  1:728540/140‑1 (MQ=255)                                                                ttCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGaa                                                     <  1:158916/140‑1 (MQ=255)                                                                   aCTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatatat                                                               >  2:516863/1‑127 (MQ=255)                                                                   aCTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCatatat                                                               <  1:516863/127‑1 (MQ=255)                                                                      gtgtCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGca                                               <  1:539512/140‑1 (MQ=255)                                                                                   cACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCg                                  >  1:281652/1‑140 (MQ=255)                                                                                   cACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCg                                  >  1:37072/1‑140 (MQ=255)                                                                                   cACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCg                                  >  2:314888/1‑140 (MQ=255)                                                                                            ttACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTc                         <  1:453082/140‑1 (MQ=255)                                                                                            ttACCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTc                         <  2:530967/140‑1 (MQ=255)                                                                                              aCCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa                       <  1:121962/140‑1 (MQ=255)                                                                                              aCCTGGCGCAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCa                       <  2:90145/140‑1 (MQ=255)                                                                                                    cgcAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAg                 <  1:96804/140‑1 (MQ=255)                                                                                                    cgcAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAg                 >  2:186653/1‑140 (MQ=255)                                                                                                      cAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCg               <  1:62177/140‑1 (MQ=255)                                                                                                      cAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCg               <  1:63853/140‑1 (MQ=255)                                                                                                      cAATATGGTGGTTGGCAACGCGCAGCAAACCGCCATCCTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCg               <  1:48819/140‑1 (MQ=255)                                                                                                             gtggtTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGagtagt        <  2:37072/140‑1 (MQ=255)                                                                                                              tggtTGGCGACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTc       >  1:453236/1‑140 (MQ=255)                                                                                                                gtTGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCa     >  1:549821/1‑140 (MQ=255)                                                                                                                  tGGCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACt   <  1:186653/140‑1 (MQ=255)                                                                                                                   ggCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACtt  >  1:720046/1‑140 (MQ=255)                                                                                                                   ggCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACtt  <  2:102416/140‑1 (MQ=255)                                                                                                                   ggCAACGCGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACtt  >  1:155612/1‑140 (MQ=255)                                                                                                                          |                                                                                                                                     GGTCATCAGTTCGATATACGGGCCGTTATTGTCGGTCAGACTCTTATCCCACGCCTGGCCAAATTCACTGTGTCCCCAACTCCACTGTTTTTTACCTGGCGCAATATGGTGGTTGGCAACGTGCAGCAAACCGCCATCTTCATCGTGACACCACGCGCCGACAAAATCGTACTGCGATTTTTCGGCCATATATGAGGTTGGAACAGGCACATTTTTATAGCGAGAAATGTCCACTCCAGCGGAGTAGTCCACTT  >  NC_000913/2681798‑2682051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑