Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,707,938	T→C	T273A (ACC→GCC)	rseB ←	anti‑sigma factor stabilizing protein RseB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,707,938	0	T	C	100.0%	104.8 / NA	31	T273A (ACC→GCC)	rseB	anti‑sigma factor stabilizing protein RseB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/18);  total (13/18)

TTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTT  >  NC_000913/2707801‑2708057                                                                                                                                          |                                                                                                                        ttGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGgcgc                                                                                                                       >  2:661124/1‑140 (MQ=255) ttGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGgcgc                                                                                                                       >  2:224472/1‑140 (MQ=255) ttGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGgcgc                                                                                                                       >  1:606812/1‑140 (MQ=255) ttGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGgcgc                                                                                                                       >  1:255380/1‑140 (MQ=255)                         ctcGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTaa                                                                                                  <  2:642537/137‑1 (MQ=255)                         ctcGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTaa                                                                                                  >  1:642537/1‑137 (MQ=255)                           cGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGttt                                                                                             >  1:632016/1‑140 (MQ=255)                           cGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGttt                                                                                             >  1:236532/1‑140 (MQ=255)                                 tGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCgag                                                                                       >  1:453408/1‑140 (MQ=255)                                    gTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAg                                                                                    <  1:661124/140‑1 (MQ=255)                                            gTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGaataa                                                                            <  1:385913/140‑1 (MQ=255)                                             tttGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGaataat                                                                           >  1:431574/1‑140 (MQ=255)                                              ttGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATc                                                                          <  1:180947/140‑1 (MQ=255)                                              ttGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATc                                                                          <  2:431574/140‑1 (MQ=255)                                                gcggcgGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCg                                                                        <  2:291971/140‑1 (MQ=255)                                                 cggcggCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGt                                                                       <  1:553605/140‑1 (MQ=255)                                                 cggcggCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGt                                                                       <  1:224472/140‑1 (MQ=255)                                                        aGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATa                                                                <  2:632016/140‑1 (MQ=255)                                                               cGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGt                                                         <  2:453408/140‑1 (MQ=255)                                                                 aCAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGa                                                       <  2:474838/140‑1 (MQ=255)                                                                   aaTGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGAtt                                                     <  2:255380/140‑1 (MQ=255)                                                                     tGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGa                                                       >  1:146606/1‑136 (MQ=255)                                                                     tGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGa                                                       <  2:146606/136‑1 (MQ=255)                                                                      ggTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGa                                                  <  1:205163/140‑1 (MQ=255)                                                                      ggTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGa                                                  >  1:225379/1‑140 (MQ=255)                                                                                    tATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTcc                                    >  1:485174/1‑140 (MQ=255)                                                                                                    aCTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGAc                    >  2:107331/1‑140 (MQ=255)                                                                                                       ggcGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTc                 <  2:528855/138‑1 (MQ=255)                                                                                                        aCGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCg                >  1:430062/1‑140 (MQ=255)                                                                                                        aCGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGGCCATCGTCGGTAGCGGACGTCg                >  1:514807/1‑140 (MQ=255)                                                                                                        aCGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCCGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAg                         <  2:50192/131‑1 (MQ=255)                                                                                                        aCGGTTCTGCGTCCGGTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCCGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAg                         >  1:50192/1‑131 (MQ=255)                                                                                                                      ggTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt  >  2:198875/1‑140 (MQ=255)                                                                                                                      ggTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt  <  1:107331/140‑1 (MQ=255)                                                                                                                      ggTGCGCAACATCTGATCGGCGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACtt  <  2:485174/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                        TTGCGCTGCCCCGAACTTAATATTCTCGGCAATGCGTTTCGCCGTTTGCGGCGGCAGTTCACCGACAATGGTGATTTCGGCGTTATCACGTACGCTTGTACTGACGGTTCTGCGTCCGGTGCGCAACATCTGATCGGTGCTCGATGGCGTAGCGCGGTTAACGTTTACCGAGAAGCTGAATAATCCGTCGGAATAGAGACGTGATTCGATAGGCATGTTGTCCATCGTCGGTAGCGGACGTCGACTACTGGAAACTT  >  NC_000913/2707801‑2708057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑