Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,827,382	C→T	T7T (ACC→ACT)	srlB →	sorbitol‑specific PTS enzyme IIA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,827,382	0	C	T	100.0%	116.3 / NA	34	T7T (ACC→ACT)	srlB	sorbitol‑specific PTS enzyme IIA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/19);  total (15/19)

GACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAGTTGAAAGGTGCA  >  NC_000913/2827247‑2827514                                                                                                                                        |                                                                                                                                     gACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGActacca                                                                                                                                  <  2:62387/140‑1 (MQ=255)          cgcgTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTa                                                                                                                         <  2:261070/140‑1 (MQ=255)                  tgtCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGcgcg                                                                                                                 >  1:125314/1‑140 (MQ=255)                           tGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCAtt                                                                                                        <  2:675310/140‑1 (MQ=255)                                  gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGc                                                                                                            >  1:150902/1‑129 (MQ=255)                                  gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGc                                                                                                            <  2:150902/129‑1 (MQ=255)                                  gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGccc                                                                                                 >  1:291966/1‑140 (MQ=255)                                  gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGccc                                                                                                 >  2:32213/1‑140 (MQ=255)                                  gTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGccc                                                                                                 >  1:35730/1‑140 (MQ=255)                                       ccGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGATTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGt                                                                                            >  2:521648/1‑140 (MQ=255)                                              ttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                                                                                     <  1:32213/140‑1 (MQ=255)                                              ttAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGa                                                                                     <  2:125314/140‑1 (MQ=255)                                                     gcgcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcat                                                                              <  1:295125/140‑1 (MQ=255)                                                       gcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcatca                                                                            >  1:391191/1‑140 (MQ=255)                                                       gcgcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCtcatca                                                                            <  2:678944/140‑1 (MQ=255)                                                         gcCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCAcc                                                                          <  2:479391/140‑1 (MQ=255)                                                                      aTCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcg                                                             <  2:171775/140‑1 (MQ=255)                                                                        cGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcgcg                                                           >  2:124708/1‑140 (MQ=255)                                                                         gCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcg                                                             <  2:16229/137‑1 (MQ=255)                                                                         gCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGgcg                                                             >  1:16229/1‑137 (MQ=255)                                                                           cTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCt                                                        >  2:258641/1‑140 (MQ=255)                                                                              gTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCg                                                     <  2:391191/140‑1 (MQ=255)                                                                                     tcCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCg                                              <  2:291966/140‑1 (MQ=255)                                                                                                        agagGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCttcattca                           >  2:32161/1‑140 (MQ=255)                                                                                                          agGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTcattcatt                         >  1:664169/1‑140 (MQ=255)                                                                                                          agGCTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGAATTGCTTcattcatt                         >  2:306363/1‑140 (MQ=255)                                                                                                             cTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCttcattca                           >  2:350464/1‑135 (MQ=255)                                                                                                             cTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCttcattca                           <  1:350464/135‑1 (MQ=255)                                                                                                             cTGAAACATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGcc                      >  2:623387/1‑140 (MQ=255)                                                                                                                aaaCATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACg                   <  2:691786/140‑1 (MQ=255)                                                                                                                  aCATGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGc                 <  1:32161/140‑1 (MQ=255)                                                                                                                     tGACCGTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAg              <  1:258641/140‑1 (MQ=255)                                                                                                                          gTTATTTATCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAGTTGaa         <  1:521648/140‑1 (MQ=255)                                                                                                                                 atCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAGTTGAAAGGTGCa  <  1:124708/140‑1 (MQ=255)                                                                                                                                 atCAGACTACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAGTTGAAAGGTGCa  <  2:664169/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GACACGGTTCGCGTCGGTGTCCCTTCTGTACTGGTGAGCCGCTTTTTAACCGGCGCGCCGACTGTACTGATCGCCTGGTTTGTCTCCGGTTTTATCTATCAATAGAGGCTGAAACATGACCGTTATTTATCAGACCACCATCACCCGTATCGGCGCGAGTGCCATTGACGCCCTCAGTGACCAGATGCTCATCACCTTTCGTGAAGGCGCGCCTGCGGACCTCGAAGAGTATTGCTTCATTCATTGCCACGGCGAGTTGAAAGGTGCA  >  NC_000913/2827247‑2827514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑