Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,839,466	T→C	intergenic (‑202/‑58)	ascG ← / → ascF	DNA‑binding transcriptional repressor AscG/beta‑glucoside specific PTS enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,839,466	0	T	C	100.0%	99.0 / NA	29	intergenic (‑202/‑58)	ascG/ascF	DNA‑binding transcriptional repressor AscG/beta‑glucoside specific PTS enzyme IIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/12);  total (17/12)

TCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACG  >  NC_000913/2839331‑2839601                                                                                                                                        |                                                                                                                                        tCGGCGGGAAAAATATTCAGGTGACCGGGTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGt                                                                                                                                     >  2:539063/1‑140 (MQ=255)      gggAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGt                                                                                                                                >  1:690313/1‑140 (MQ=255)                   aGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATaacaa                                                                                                                   >  2:426588/1‑140 (MQ=255)                     gTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACt                                                                                                                 <  1:426588/140‑1 (MQ=255)                       gACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTAc                                                                                                               <  2:690313/140‑1 (MQ=255)                          cGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATc                                                                                                            >  1:674371/1‑140 (MQ=255)                              ttCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTAcc                                                                                                        >  2:396185/1‑140 (MQ=255)                              ttCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTAcc                                                                                                        <  1:539063/140‑1 (MQ=255)                                      tataAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGAt                                                                                                <  1:477231/140‑1 (MQ=255)                                               tGAACAACTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATa                                                                                       >  1:52121/1‑140 (MQ=255)                                                gAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATaa                                                                                      >  1:117237/1‑140 (MQ=255)                                                                           tGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATgcggcg                                                           >  2:60007/1‑140 (MQ=255)                                                                               aaCTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTgg                                                       <  2:585419/140‑1 (MQ=255)                                                                                 cTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCa                                                     <  1:604182/140‑1 (MQ=255)                                                                                    ttCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGc                                                  >  2:376981/1‑140 (MQ=255)                                                                                      cATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTc                                                >  1:646553/1‑140 (MQ=255)                                                                                              tgtgAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAg                                        >  2:339277/1‑140 (MQ=255)                                                                                              tgtgAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAg                                        >  1:284674/1‑140 (MQ=255)                                                                                              tgtgAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAg                                        >  2:576184/1‑140 (MQ=255)                                                                                                        gTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTggg                              >  2:166561/1‑140 (MQ=255)                                                                                                        gTTTCTTAATTCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTggg                              <  1:396185/140‑1 (MQ=255)                                                                                                                  tCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATGACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGAt                    >  2:677582/1‑140 (MQ=255)                                                                                                                  tCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGggcg                            <  1:490627/132‑1 (MQ=255)                                                                                                                  tCCGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGggcg                            >  2:490627/1‑132 (MQ=255)                                                                                                                   ccGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACt                                 <  2:273345/126‑1 (MQ=255)                                                                                                                   ccGTTTCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACt                                 >  1:273345/1‑126 (MQ=255)                                                                                                                      tttCAGCATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACa                >  2:207476/1‑140 (MQ=255)                                                                                                                            cctCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTcgg          <  2:674371/138‑1 (MQ=255)                                                                                                                            cATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTcgg          <  1:60007/140‑1 (MQ=255)                                                                                                                            cATCGGCATTTTCCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTcgg          <  1:472372/140‑1 (MQ=255)                                                                                                                                    ttttcCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACg  <  1:339277/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCACGTCGACTGATAACAACTACATCTACCCTACTGATAACAGGATAAAATCCGATGGCCAAAAATTATGCGGCGCTGGCACGCTCGGTGATAGCGGCACTGGGCGGCGTTGATAACATCTCGGCGGTCACG  >  NC_000913/2839331‑2839601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑