Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,965,300	G→A	R246R (CGC→CGT)	lgt ←	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,965,300	0	G	A	100.0%	176.9 / NA	50	R246R (CGC→CGT)	lgt	phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/24);  total (26/24)

GAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGGCGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCCAGCAGC  >  NC_000913/2965166‑2965429                                                                                                                                       |                                                                                                                                  gAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGaa                                                                                                                              >  1:289121/1‑140 (MQ=255)                    tGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGa                                                                                                           >  1:706624/1‑140 (MQ=255)                                 ccAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAAc                                                                                              <  1:594891/140‑1 (MQ=255)                                     aCCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAAt                                                                                          >  1:613314/1‑140 (MQ=255)                                     aCCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAAt                                                                                          >  2:685077/1‑140 (MQ=255)                                     aCCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAAt                                                                                          >  1:621579/1‑140 (MQ=255)                                     aCCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAAt                                                                                          >  1:682695/1‑140 (MQ=255)                                          catGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGa                                                                                     >  1:395549/1‑140 (MQ=255)                                          catGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGa                                                                                     >  2:173347/1‑140 (MQ=255)                                          catGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGa                                                                                     >  1:443214/1‑140 (MQ=255)                                          catGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGa                                                                                     >  2:222827/1‑140 (MQ=255)                                          catGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGa                                                                                     >  1:290692/1‑140 (MQ=255)                                            tGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAAc                                                                                   <  2:423076/140‑1 (MQ=255)                                            tGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAAc                                                                                   <  2:613314/140‑1 (MQ=255)                                            tGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAAc                                                                                   <  1:222827/140‑1 (MQ=255)                                                   acCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTg                                                                            >  2:89812/1‑140 (MQ=255)                                                     ccGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTgag                                                                          <  2:290692/140‑1 (MQ=255)                                                     ccGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTgag                                                                          <  1:173347/140‑1 (MQ=255)                                                     ccGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTgag                                                                          >  2:54579/1‑140 (MQ=255)                                                       gcgACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGAc                                                                        >  1:713461/1‑140 (MQ=255)                                                                     gATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGc                                                          <  1:57199/140‑1 (MQ=255)                                                                      aTGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGTGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCg                                                         <  1:698931/140‑1 (MQ=255)                                                                      aTGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCg                                                         <  1:89812/140‑1 (MQ=255)                                                                       tGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGt                                                        <  2:682695/140‑1 (MQ=255)                                                                       tGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGt                                                        <  2:621579/140‑1 (MQ=255)                                                                           aaGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGtt                                                    <  1:54579/140‑1 (MQ=255)                                                                             gAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTa                                                  <  2:706624/140‑1 (MQ=255)                                                                             gAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTa                                                  <  1:94501/140‑1 (MQ=255)                                                                             gAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTa                                                  <  2:289121/140‑1 (MQ=255)                                                                               aTTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACg                                                <  1:388410/140‑1 (MQ=255)                                                                                tttGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGa                                               <  2:443214/140‑1 (MQ=255)                                                                                    ccccATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCg                                                         <  2:530225/126‑1 (MQ=255)                                                                                    ccccATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCg                                                         >  1:530225/1‑126 (MQ=255)                                                                                                gTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGa                               <  1:546521/140‑1 (MQ=255)                                                                                                    tGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGataat                           >  1:406946/1‑140 (MQ=255)                                                                                                     gCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGataata                          <  2:713461/140‑1 (MQ=255)                                                                                                       aCCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATaaa                        <  2:368437/140‑1 (MQ=255)                                                                                                       aCCCAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATaaa                        <  1:685077/140‑1 (MQ=255)                                                                                                        cccAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATAc                                         <  2:131706/122‑1 (MQ=255)                                                                                                        cccAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATAc                                         >  1:131706/1‑122 (MQ=255)                                                                                                        cccAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAAc                       >  2:167568/1‑140 (MQ=255)                                                                                                        cccAGGCACCGGTAAACTGCGCGTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGAC‑GCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACa                      >  1:418274/1‑140 (MQ=255)                                                                                                                           cgcgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagca    >  1:389904/1‑140 (MQ=255)                                                                                                                           cgcgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagca    >  1:9247/1‑140 (MQ=255)                                                                                                                           cgcgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagca    >  1:688161/1‑140 (MQ=255)                                                                                                                           cgcgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagca    >  1:248067/1‑140 (MQ=255)                                                                                                                            gcgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagcag   <  1:299733/140‑1 (MQ=255)                                                                                                                             cgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagcagc  >  2:271192/1‑140 (MQ=255)                                                                                                                             cgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagcagc  >  2:561904/1‑140 (MQ=255)                                                                                                                             cgTCGGGCTGACGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCcagcagc  >  2:579274/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                  GAAACGTGTTGCTGTGGGCTGCGACGATATGCCCAGACCATCATGATCACACCCGCGACAATCATCGGGATGGAAAGAATTTGCCCCATGCTGATGTACTGCACCCAGGCACCGGTAAACTGCGCGTCGGGCTGGCGGAAAAACTCAACAATGATGCGAAACGCGCCGTAACCAATCAGGAACAAACCTGAGACAGCTCCCATTGGGCGTGGTTTACGAATATACAGGTTGAGGATAATAAACAGCACCACACCTTCCAGCAGC  >  NC_000913/2965166‑2965429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑