Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,031,485	T→C	V40A (GTC→GCC)	uacT →	urate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,031,485	0	T	C	100.0%	159.9 / NA	46	V40A (GTC→GCC)	uacT	urate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/24);  total (22/24)

ACACTGATGAGCGCCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGG  >  NC_000913/3031361‑3031617                                                                                                                             |                                                                                                                                     acacTGATGAGCGCCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGc                                                                                                                       <  2:420906/140‑1 (MQ=255) acacTGATGAGCGCCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGc                                                                                                                       <  1:614695/140‑1 (MQ=255) acacTGATGAGCGCCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGc                                                                                                                       <  1:512139/140‑1 (MQ=255)    cTGATGAGCGCCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGt                                                                                                                    <  1:691076/140‑1 (MQ=255)           gcgcCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGtt                                                                                                             <  2:594414/140‑1 (MQ=255)               cATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGc                                                                                                                 <  2:589382/132‑1 (MQ=255)               cATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGc                                                                                                                 >  1:589382/1‑132 (MQ=255)                       cccAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGAtt                                                                                                 >  2:28502/1‑140 (MQ=255)                       cccAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGAtt                                                                                                 >  2:696002/1‑140 (MQ=255)                             ttCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGAc                                                                                           >  2:292633/1‑140 (MQ=255)                               cccTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCg                                                                                         >  1:574569/1‑140 (MQ=255)                                              cAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCaaa                                                                          >  1:563473/1‑140 (MQ=255)                                                aGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAaag                                                                        <  2:574569/140‑1 (MQ=255)                                                 gACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAaaga                                                                       >  1:441384/1‑140 (MQ=255)                                                 gACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAaaga                                                                       <  2:137653/140‑1 (MQ=255)                                                 gACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAaaga                                                                       >  2:466789/1‑140 (MQ=255)                                                      cccAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTa                                                                  >  2:720139/1‑140 (MQ=255)                                                           cTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCg                                                             >  2:423843/1‑140 (MQ=255)                                                                  ggTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCa                                                      <  2:441384/140‑1 (MQ=255)                                                                   gTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCAt                                                     <  1:423843/140‑1 (MQ=255)                                                                             tattatCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGAt                                           >  2:364263/1‑140 (MQ=255)                                                                             tattatCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGAt                                           >  1:282995/1‑140 (MQ=255)                                                                                  tCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAg                                                  <  2:352452/128‑1 (MQ=255)                                                                                  tCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAg                                                  >  1:352452/1‑128 (MQ=255)                                                                                         gAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCgg                                             >  2:595296/1‑126 (MQ=255)                                                                                         gAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCgg                                             <  1:595296/126‑1 (MQ=255)                                                                                         gAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCGCTTTtgctgc                               >  1:679780/1‑140 (MQ=255)                                                                                            aGCTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCggg                            >  1:3422/1‑140 (MQ=255)                                                                                              cTGATCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGAt                          <  1:466789/140‑1 (MQ=255)                                                                                                 aTCATACTCGGTCTGCAACACGTCCTTGCCATGTACGGAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGt                       <  1:13059/140‑1 (MQ=255)                                                                                                 aTCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGt                       <  1:364263/140‑1 (MQ=255)                                                                                                 aTCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGt                       <  2:140389/140‑1 (MQ=255)                                                                                                  tCATACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTc                      >  1:327861/1‑140 (MQ=255)                                                                                                     tACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTcaca                   <  1:696002/140‑1 (MQ=255)                                                                                                     tACTCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTcaca                   <  1:28502/140‑1 (MQ=255)                                                                                                        tCGGTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACatta                <  2:282995/140‑1 (MQ=255)                                                                                                           gTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACatta                <  2:414296/137‑1 (MQ=255)                                                                                                           gTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACatta                >  1:414296/1‑137 (MQ=255)                                                                                                           gTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTg             >  2:611624/1‑140 (MQ=255)                                                                                                           gTCTGCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTg             <  2:238335/140‑1 (MQ=255)                                                                                                               gCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTg             >  2:84771/1‑136 (MQ=255)                                                                                                               gCAACACGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTg             <  1:84771/136‑1 (MQ=255)                                                                                                                  acacGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATgta      <  1:720139/140‑1 (MQ=255)                                                                                                                     cGTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATgtatcg   <  2:563473/140‑1 (MQ=255)                                                                                                                      gTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATgtatcgg  >  1:333977/1‑140 (MQ=255)                                                                                                                      gTCCTTGCCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATgtatcgg  >  1:105955/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                     ACACTGATGAGCGCCATAGATTCCCAACTTCCCTCATCTTCTGGGCAAGACCGCCCAACTGATGAGGTTGACCGCATATTATCACCAGGAAAGCTGATCATACTCGGTCTGCAACACGTCCTTGTCATGTACGCAGGTGCAGTCGCTGTTCCTCTTATGATTGGTGACCGACTGGGCCTCTCAAAAGAAGCTATTGCGATGCTCATTAGCTCGGATCTCTTTTGCTGCGGGATCGTCACATTATTGCAATGTATCGG  >  NC_000913/3031361‑3031617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑