Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,297	A→G	D104G (GAT→GGT)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,297	0	A	G	100.0%	128.5 / NA	37	D104G (GAT→GGT)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

GCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGAC  >  NC_000913/3063168‑3063430                                                                                                                                  |                                                                                                                                      gCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGc                                                                                                                             >  1:188683/1‑140 (MQ=255)  cACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCa                                                                                                                            >  2:131794/1‑140 (MQ=255)     ccccGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCatga                                                                                                                         <  1:108446/140‑1 (MQ=255)     ccccGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCatga                                                                                                                         <  2:279045/140‑1 (MQ=255)       ccGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCatgaat                                                                                                                       >  2:102493/1‑140 (MQ=255)             cgGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTg                                                                                                                 >  2:227285/1‑140 (MQ=255)               gggAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGgc                                                                                                               <  1:497099/140‑1 (MQ=255)                          cTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAg                                                                                                    <  1:203368/140‑1 (MQ=255)                                 gAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAg                                                                                                    >  2:673825/1‑133 (MQ=255)                                 gAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAg                                                                                                    <  1:673825/133‑1 (MQ=255)                                         tGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGcc                                                                                     <  2:188683/140‑1 (MQ=255)                                         tGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGcc                                                                                     >  1:630211/1‑140 (MQ=255)                                              tgttgtTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTa                                                                                <  1:298653/140‑1 (MQ=255)                                                      aTTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCAtcctc                                                                        >  1:447524/1‑140 (MQ=255)                                                        tCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCg                                                                      <  1:138024/140‑1 (MQ=255)                                                        tCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCg                                                                      <  1:102493/140‑1 (MQ=255)                                                          gagaGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGt                                                                    <  2:447524/140‑1 (MQ=255)                                                          gagaGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTTGCCCGGGACCATCCTCCggg                                                                    >  2:57483/1‑139 (MQ=255)                                                                          cgcgGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCa                                                    >  2:568014/1‑140 (MQ=255)                                                                           gcgGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGGACCCTCCTCCGGGCATCTGGGCGGGGCCAg                                                   >  2:136779/1‑140 (MQ=255)                                                                                       gTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGcgcg                                       <  1:57483/140‑1 (MQ=255)                                                                                       gTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGcgcg                                       <  1:131794/140‑1 (MQ=255)                                                                                         cGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCagc                                              >  1:275151/1‑131 (MQ=255)                                                                                         cGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCagc                                              <  2:275151/131‑1 (MQ=255)                                                                                          gATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGa                                    <  1:410457/140‑1 (MQ=255)                                                                                            tCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAAt                                  <  2:484896/140‑1 (MQ=255)                                                                                                agcagcCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAAt                              >  2:679727/1‑140 (MQ=255)                                                                                                 gcagcCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATg                             <  1:568014/140‑1 (MQ=255)                                                                                                  cagcCCGGTCACTGGCGGTAGCATTCTCGGGGGGAAAACCCGCATGAAAGACCCGGCGCGGGCCGAAGCGGCGGTTATTTGCCCGGTACCATCCTCCGGTCATCTGGGGGGGGCCAGTCAGCGAGCGCGGGGATTAATGc                            >  2:532351/1‑140 (MQ=255)                                                                                                    gcCCGGTCACTGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTg                          <  1:227285/140‑1 (MQ=255)                                                                                                              tGGCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAag                >  1:675462/1‑140 (MQ=255)                                                                                                                 cGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAagcag             <  1:679727/140‑1 (MQ=255)                                                                                                                 cGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAagcag             <  2:630211/140‑1 (MQ=255)                                                                                                                  ggTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAagcagc            <  1:532351/140‑1 (MQ=255)                                                                                                                          tctcGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATg    >  1:683751/1‑140 (MQ=255)                                                                                                                           ctcGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGa   >  1:416199/1‑140 (MQ=255)                                                                                                                            tcGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGAc  >  2:537481/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                      GCACCCCCGGCGCGGGGAAAAGTACCTTTCTTGAGGCCTTTGGCATGTTGTTGATTCGAGAGGGATTAAAGGTCGCGGTTATTGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGAC  >  NC_000913/3063168‑3063430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑