Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,696,365	(G)6→7	intergenic (‑294/+97)	uidA ← / ← uidR	beta‑glucuronidase/DNA‑binding transcriptional repressor UidR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,696,359	1	.	G	100.0%	100.9 / NA	29	intergenic (‑288/+103)	uidA/uidR	beta‑glucuronidase/DNA‑binding transcriptional repressor UidR
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (15/14);  total (15/14)

AGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTT‑GGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCG  >  NC_000913/1696232‑1696493                                                                                                                                 |                                                                                                                                       aGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATtc                                                                                                                             >  1:180435/1‑140 (MQ=255)    gTGATATAGATCACATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTcc                                                                                                                          >  1:85946/1‑140 (MQ=255)                gCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGAGAATATTTTGGGGGGGAATTCCCCGAAGCCGGGAAg                                                                                                              >  1:264580/1‑140 (MQ=255)                  aTTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGa                                                                                                            >  1:427043/1‑140 (MQ=255)                  aTTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGa                                                                                                            >  1:524013/1‑140 (MQ=255)                    tAATCTTTAAAACAGCGTTGACAGAAGCCATAAGATAAGCAACGGAACGTTCGTTCGAGCGGATTGGGAACGATAAATTCTCATCACATATGCCTACTGTGTATATTTTGGGGGGGAATTCTCCGAAGCCGTGAAGGATa                                                                                                          >  2:28752/1‑140 (MQ=255)                         tttAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAAtttt                                                                                                     >  1:29368/1‑140 (MQ=255)                                   cGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGcaca                                                                                               <  2:456257/136‑1 (MQ=255)                                   cGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGcaca                                                                                               >  1:456257/1‑136 (MQ=255)                                   cGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAgaga                                                                                           >  1:470755/1‑140 (MQ=255)                                          aGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACg                                                                                    <  2:146702/140‑1 (MQ=255)                                          aGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACg                                                                                    <  2:295356/140‑1 (MQ=255)                                                  tAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGaaa                                                                            <  1:117143/140‑1 (MQ=255)                                                          gCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGca                                                                    <  2:85946/140‑1 (MQ=255)                                                          gCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGca                                                                    <  1:277912/140‑1 (MQ=255)                                                          gCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGca                                                                    <  1:19787/140‑1 (MQ=255)                                                              ccGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGt                                                                >  1:489682/1‑140 (MQ=255)                                                                           cgAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCccg                                                   <  2:182579/140‑1 (MQ=255)                                                                            gAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCccgc                                                  >  1:496764/1‑140 (MQ=255)                                                                                            aTAAACTCTCATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGaa                                  >  2:97850/1‑140 (MQ=255)                                                                                                   ctcATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt                           <  1:97850/140‑1 (MQ=255)                                                                                                   ctcATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt                           <  1:362512/140‑1 (MQ=255)                                                                                                   ctcATGACATATGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGAt                           <  1:199200/140‑1 (MQ=255)                                                                                                           atatGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTaa                   >  2:490860/1‑140 (MQ=255)                                                                                                             atGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGa                 <  2:29368/140‑1 (MQ=255)                                                                                                              tGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGa                 >  2:24163/1‑139 (MQ=255)                                                                                                              tGGCTACAGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGa                 <  1:24163/139‑1 (MQ=255)                                                                                                                     aGTGAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCa         >  2:446571/1‑140 (MQ=255)                                                                                                                        gAATATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatc      >  2:187608/1‑140 (MQ=255)                                                                                                                         aaTATTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatca     >  1:416597/1‑140 (MQ=255)                                                                                                                          atatTTTGGGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAatcat    <  2:120752/140‑1 (MQ=255)                                                                                                                           attttg‑GGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATcg  <  2:524013/138‑1 (MQ=255)                                                                                                                                 |                                                                                                                                       AGCGTGATATAGATCGCATTAATCTTTAAAACAGCGTTGACAGAAGCCATAAAATAAGCAACCGAACGTTCGTTCGAGCGTATTGGGAACGATAAACTCTCATGACATATGGCTACAGTGAATATTTT‑GGGGGGAATTCTCCGAAGCCGGGAAGGATAATTTTTGCACAGAGAAATTACGATATGAAACGCGGGCACAGTTATCACCAGCCCGCCGAAAAAGAGAGAGAATCAGGATGCGGTTAAGATACCGCCAATCATCG  >  NC_000913/1696232‑1696493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑