| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,462,889 | (C)9→10 | intergenic (+243/‑123) | fadL → / → yfdF | long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,462,880 | 1 | . | C | 93.5% | 97.4 / 0.5 | 31 | intergenic (+234/‑132) | fadL/yfdF | long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (15/14); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
ACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAAT > NC_000913/2462749‑2463000 | aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑cccccccc < 2:520908/140‑1 (MQ=255)aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑cccccccc < 1:20442/140‑1 (MQ=255)aCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑cccccccc > 2:294787/1‑140 (MQ=255) aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATtgtg > 2:56152/1‑140 (MQ=255) aGCTTGCCCTTTCAAATCAATAAGCAGTATCCACAGACAAGCCTGAAGGAAAAGGCATATTTTCAGGCGTTCTGCGCGTGCTTCTCAAAGAGTTTTCTTTGCTGCATTGCCAGGATGCCC‑CCCCCCCCCTGGCTATtgtg < 2:138531/140‑1 (MQ=255) gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTgc > 2:44683/1‑140 (MQ=255) cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCATCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTgcg > 2:533825/1‑140 (MQ=255) aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTgcg > 1:186809/1‑124 (MQ=255) aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTgcg < 2:186809/124‑1 (MQ=255) gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa < 1:258525/140‑1 (MQ=255) tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt < 1:44683/140‑1 (MQ=255) tCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt < 1:23201/140‑1 (MQ=255) ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTc > 1:211094/1‑140 (MQ=255) gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat > 2:470102/1‑140 (MQ=255) aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt < 2:345081/135‑1 (MQ=255) aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCt > 1:345081/1‑135 (MQ=255) aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTaaataaat > 2:498358/1‑140 (MQ=255) cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg < 1:374939/140‑1 (MQ=255) cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg < 1:294787/140‑1 (MQ=255) gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg < 1:56152/140‑1 (MQ=255) aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa > 2:351794/1‑140 (MQ=255) aGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa < 2:211094/140‑1 (MQ=255) cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg < 2:339163/140‑1 (MQ=255) aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt > 1:307026/1‑140 (MQ=255) ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg < 1:351794/140‑1 (MQ=255) tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTTGTTGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAAGGAGCGTTTTTTGATAGTCTTTTTCATTAggg > 2:382855/1‑139 (MQ=255) tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt > 1:105789/1‑140 (MQ=255) tctcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTg > 1:152441/1‑140 (MQ=255) tcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa > 2:533530/1‑140 (MQ=255) tcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa < 1:470102/140‑1 (MQ=255) tttACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatca > 1:364015/1‑140 (MQ=255) ttACTTTTCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACATTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaa > 2:42923/1‑140 (MQ=255) tACTTTTCTGCATTTCCAGGAT‑ACT‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAaatcaatc > 2:142253/1‑140 (MQ=255) ttCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCaaaa < 1:533825/140‑1 (MQ=255) tCTGCATTTCCAGGAT‑ACTCCCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAAt < 2:152441/140‑1 (MQ=255) | ACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑CCCCCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAAT > NC_000913/2462749‑2463000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |