Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,993	(T)5→6	coding (752/1332 nt)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,988	1	.	T	100.0%	102.5 / NA	30	R249R (CGC→CGT)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (16/14);  total (16/14)

AGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949852‑2950122                                                                                                                                          |                                                                                                                                        aGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑ttt                                                                                                                                      >  2:441471/1‑140 (MQ=255)     gatTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑tttt‑ttg                                                                                                                                 >  1:451767/1‑138 (MQ=255)            ttttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGgcg                                                                                                                           <  1:17618/140‑1 (MQ=255)                    ttCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGaaa                                                                                                                   >  2:509699/1‑140 (MQ=255)                         aGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTg                                                                                                              >  1:429167/1‑140 (MQ=255)                                cACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAgc                                                                                                       >  1:146320/1‑140 (MQ=255)                                          gacgGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTc                                                                                             >  2:346670/1‑140 (MQ=255)                                             ggTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCt                                                                                          <  1:441471/140‑1 (MQ=255)                                                      gTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTaa                                                                                 <  2:2839/140‑1 (MQ=255)                                                       tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcgt                                                                                              >  1:483825/1‑126 (MQ=255)                                                       tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGgcgtgcgt                                                                                              <  2:483825/126‑1 (MQ=255)                                                       tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  1:141563/140‑1 (MQ=255)                                                       tctcCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAAt                                                                                <  2:433790/140‑1 (MQ=255)                                                                        aaCGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATg                                                               >  1:123774/1‑140 (MQ=255)                                                                           gAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGgcg                                                            <  2:451767/140‑1 (MQ=255)                                                                                    gcgcGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGc                                                   <  2:429167/140‑1 (MQ=255)                                                                                              aaGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        >  1:45372/1‑125 (MQ=255)                                                                                              aaGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCt                                                        <  2:45372/125‑1 (MQ=255)                                                                                                   cAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAc                                    >  2:409798/1‑140 (MQ=255)                                                                                                     ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  >  1:460734/1‑140 (MQ=255)                                                                                                     ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  <  2:146320/140‑1 (MQ=255)                                                                                                     ggAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCAcgc                                  >  1:213060/1‑140 (MQ=255)                                                                                                       aaGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGa                                <  1:409798/140‑1 (MQ=255)                                                                                                        agagAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGAc                               <  2:213060/140‑1 (MQ=255)                                                                                                           gaAAGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGt                            >  2:57777/1‑140 (MQ=255)                                                                                                            aaaGGCGATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTa                           >  1:521303/1‑140 (MQ=255)                                                                                                                  gATTACAACTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTa                     >  2:201542/1‑140 (MQ=255)                                                                                                                        aaCTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  1:509699/140‑1 (MQ=255)                                                                                                                        aaCTCCGGTACGGTGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  1:1447/140‑1 (MQ=255)                                                                                                                        aaCGCCGGTACGGTGCGC‑TTTTGTGCGTGGCGCAGAGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGa               <  1:84086/140‑1 (MQ=255)                                                                                                                                     tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:53833/1‑140 (MQ=255)                                                                                                                                     tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:395715/1‑140 (MQ=255)                                                                                                                                     tGCGCTTTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAg  >  1:171669/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        AGATGATTGGTTTTTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGC‑TTTT‑TGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTTAATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAAG  >  NC_000913/2949852‑2950122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑