Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,003,479	(G)6→7	coding (866/879 nt)	xdhB →	putative xanthine dehydrogenase FAD‑binding subunit XdhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,003,473	1	.	G	100.0%	48.4 / NA	15	A287G (GCG→GGG)	xdhB	putative xanthine dehydrogenase FAD‑binding subunit XdhB
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (10/5);  total (10/5)

CAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCTA  >  NC_000913/3003337‑3003602                                                                                                                                          |                                                                                                                                  cAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑ggg                                                                                                                                <  1:14176/140‑1 (MQ=255)                          cTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGc                                                                                                        <  2:158921/140‑1 (MQ=255)                           tGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCg                                                                                                       <  1:390784/140‑1 (MQ=255)                                                                gCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGGAAATTGCAATGAAACACAGCGAAACAATTACCATCGAATGCACCCTTAACGGGGTGcc                                                                  >  1:23636/1‑140 (MQ=255)                                                                           gTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTc                                                       >  1:524665/1‑140 (MQ=255)                                                                              tCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCAcg                                                    >  1:279647/1‑140 (MQ=255)                                                                                           aTCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCAc                                       >  2:68055/1‑140 (MQ=255)                                                                                                   gATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGctct                               <  2:524665/140‑1 (MQ=255)                                                                                                     tGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCAcg                                      >  1:364599/1‑131 (MQ=255)                                                                                                     tGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCAcg                                      <  2:364599/131‑1 (MQ=255)                                                                                                     tGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCg                             >  1:325654/1‑140 (MQ=255)                                                                                                       aCCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGa                           >  2:526132/1‑140 (MQ=255)                                                                                                           aaaaaGTGATTAGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTa                       >  2:361055/1‑140 (MQ=255)                                                                                                                      aGCGAAGCCGTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACa            >  1:431385/1‑140 (MQ=255)                                                                                                                               gTCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATACCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCt   <  1:241024/140‑1 (MQ=255)                                                                                                                                tCGCCGCGGCGGGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCTa  >  1:336122/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                  CAAACGCTGGAAGCTATCAGCGAATCTGTCCTGCAAGATGTCGCCCCGCGTTCTTCATGGCGGGCCAGTAAAGAGTTTCGTCTGCATCTCATCCAGACGATGACCAAAAAAGTGATTAGCGAAGCCGTCGCCGCGGC‑GGGGGGAAAATTGCAATGAATCACAGCGAAACAATTACCATCGAATGCACCATTAACGGGATGCCTTTTCAGCTTCACGCCGCACCAGGCACGCCGCTCTCGGAATTACTCCGCGAACAAGGACTGCTA  >  NC_000913/3003337‑3003602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑