| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 445,558 | 0 | A | C | 54.5% | ‑6.0 / 11.3 | 11 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base C (0/6); total (4/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGG > NC_000913/445420‑445696 | aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCTTGGGACTGGCGGCCGTTAGCCACACTGCGGCCCGCATTGCGCCCGCGAGAAAAAc > 2:58748/1‑140 (MQ=255) cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGGCATGCGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACa < 1:478290/140‑1 (MQ=255) tGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATTGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAAAACCCCCTGACCCTCAAACATGCCGTTaa > 2:356899/1‑140 (MQ=255) tGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAAACCCCCGACCGTCAAACAAGCCGTTaa > 2:474784/1‑140 (MQ=255) cGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGGCATACGGCGGTTCTTTCATGGTACTGGCCACTGGCAGCCCCACCCCGGCCAGCATTTCGCCAGCGAGAAATACCCCCTGACCCTCAAACAAGCCCTTAATc > 2:478290/1‑140 (MQ=255) aCATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGGCAGCATTGCGCCCGCGAGAAATAACCCCTGACCGGCAAACAAGCCGTTAATCCAGCCGCCCCGCGAACCGCCAAACGCCACCCCCAGaaa > 2:161130/1‑140 (MQ=255) ggtttttttCAGGGTACGGGCGACTGTCACCCACACTGCGGCCAGCATGGCGCCAGCGAGAAATCCCCCCTGCCCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggctgg < 2:445708/134‑1 (MQ=255) gTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAATCGCCCCGCCAAGAAActggctgggg > 2:154880/1‑138 (MQ=255) gTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTTCGCCAGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAAACCCCACGCCCAGAAActggctgggg > 2:232375/1‑138 (MQ=255) cATGGTACGGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaaa < 1:161130/140‑1 (MQ=255) aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa > 2:48939/1‑140 (MQ=255) gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc > 1:89956/1‑140 (MQ=255) gccTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg < 1:154880/138‑1 (MQ=255) cTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa < 1:444285/140‑1 (MQ=255) tCAGCCACACGGCGGCAAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt < 1:492030/140‑1 (MQ=255) cacTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAc > 1:115786/1‑140 (MQ=255) gCCAGCATTGCCCCGGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg < 1:232375/140‑1 (MQ=255) gCATGGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc < 1:140749/140‑1 (MQ=255) cttggcgcCGGGGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct < 2:115786/136‑1 (MQ=255) aaaaTCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt < 1:474784/139‑1 (MQ=255) aaaaTCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt < 1:356899/139‑1 (MQ=255) tACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAgg > 2:24134/1‑140 (MQ=255) | AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGG > NC_000913/445420‑445696 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |