Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	190,530	A→G	A219A (GCA→GCG)	rpsB →	30S ribosomal subunit protein S2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	190,530	0	A	G	100.0%	143.2 / NA	41	A219A (GCA→GCG)	rpsB	30S ribosomal subunit protein S2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/18);  total (23/18)

AAGAAGCAAACAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCAACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAATTCCCG  >  NC_000913/190394‑190664                                                                                                                                         |                                                                                                                                       aagaagCAAACAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGAcc                                                                                                                                     >  2:210203/1‑140 (MQ=255)  agaagCAAACAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCg                                                                                                                                    <  2:174350/140‑1 (MQ=255)     agCAAACAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTAc                                                                                                                                 >  1:79109/1‑140 (MQ=255)       cAAACAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGt                                                                                                                               >  1:186538/1‑140 (MQ=255)        aaaCAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTg                                                                                                                              >  2:498352/1‑140 (MQ=255)            aacCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAgg                                                                                                                          <  1:482201/140‑1 (MQ=255)             acCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGc                                                                                                                         <  2:79109/140‑1 (MQ=255)                 gggTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGA‑CGTACGTGAAGGCCGTtc                                                                                                                    >  1:362412/1‑140 (MQ=255)                       tCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAgg                                                                                                               >  2:329418/1‑140 (MQ=255)                                 gCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGA‑CGTACGTGAAGGCCGTTCTCAGGATCTGGCTTcc                                                                                                    <  2:362412/140‑1 (MQ=255)                                            tACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGgaag                                                                                          >  1:92941/1‑140 (MQ=255)                                              ccAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGgaagaa                                                                                        <  1:214175/140‑1 (MQ=255)                                               cAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGaaa                                                                                       >  1:220723/1‑140 (MQ=255)                                                  ctctGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCt                                                                                    >  1:3164/1‑140 (MQ=255)                                                       aTCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAGTCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGGGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTa                                                                               >  1:385900/1‑140 (MQ=255)                                                          cGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGaa                                                                            <  2:18182/140‑1 (MQ=255)                                                           ggACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAGTCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGGGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGTCGGAAGAAAGCTTCGTAGAAg                                                                           <  2:385900/140‑1 (MQ=255)                                                           ggACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAg                                                                           <  2:127994/140‑1 (MQ=255)                                                            gACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGc                                                                          >  2:318820/1‑140 (MQ=255)                                                             aCGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCt                                                                         <  2:344257/140‑1 (MQ=255)                                                              cGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTg                                                                        <  2:223256/140‑1 (MQ=255)                                                                           gTTATCCCGGGTAACGACGACGCAATTCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCtt                                                           >  1:462602/1‑140 (MQ=255)                                                                               tCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTg                                                                        <  1:461022/123‑1 (MQ=255)                                                                               tCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTg                                                                        >  2:461022/1‑123 (MQ=255)                                                                                   gggTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTc                                                   <  1:210203/140‑1 (MQ=255)                                                                                    ggTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTcc                                                  >  1:378675/1‑140 (MQ=255)                                                                                              acgCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAg                                        >  1:451495/1‑140 (MQ=255)                                                                                               cgCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGt                                       >  1:536739/1‑140 (MQ=255)                                                                                                gCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGtt                                      >  1:212475/1‑140 (MQ=255)                                                                                                gCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGtt                                      <  2:220723/140‑1 (MQ=255)                                                                                                 cAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACGCCCCCAAAATAGTTc                                     <  2:364606/140‑1 (MQ=255)                                                                                                   aTCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGa                                   <  1:232934/140‑1 (MQ=255)                                                                                                    tCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAg                                  <  1:498352/140‑1 (MQ=255)                                                                                                                   tGTACCTGGGCACTGTGGCTGAGAGCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGGTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGGTCGAGTTGCAGAAAggcggc                   <  2:388060/140‑1 (MQ=255)                                                                                                                          gggCGCTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCg            >  2:196857/1‑140 (MQ=255)                                                                                                                              gcTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGaa        <  2:378675/140‑1 (MQ=255)                                                                                                                               cTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAAt       >  1:64384/1‑140 (MQ=255)                                                                                                                               cTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAAt       >  2:24318/1‑140 (MQ=255)                                                                                                                               cTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAAt       >  2:343954/1‑140 (MQ=255)                                                                                                                               cTGTTGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAAt       >  2:102461/1‑140 (MQ=255)                                                                                                                                  ttGCTGCGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAATTcc    >  2:194356/1‑140 (MQ=255)                                                                                                                                    gctgcGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAATTCCCg  <  1:415960/140‑1 (MQ=255)                                                                                                                                    gctgcGACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAATTCCCg  <  1:279186/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AAGAAGCAAACAACCTGGGTATTCCGGTATTTGCTATCGTTGATACCAACTCTGATCCGGACGGTGTTGACTTCGTTATCCCGGGTAACGACGACGCAATCCGTGCTGTGACCCTGTACCTGGGCGCTGTTGCTGCAACCGTACGTGAAGGCCGTTCTCAGGATCTGGCTTCCCAGGCGGAAGAAAGCTTCGTAGAAGCTGAGTAATAAGGCTTGATAACTCCCCCAAAATAGTTCGAGTTGCAGAAAGGCGGCAAGCTCGAGAATTCCCG  >  NC_000913/190394‑190664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑