Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,885,620	A→G	N182S (AAC→AGC)	yidC →	membrane protein insertase YidC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,885,620	0	A	G	100.0%	143.5 / NA	41	N182S (AAC→AGC)	yidC	membrane protein insertase YidC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/17);  total (24/17)

TGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGA  >  NC_000913/3885494‑3885755                                                                                                                               |                                                                                                                                        tggctgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGaaa                                                                                                                            >  1:214214/1‑140 (MQ=255)    ctgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCg                                                                                                                         <  1:198164/140‑1 (MQ=255)     tgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGc                                                                                                                        <  2:545956/140‑1 (MQ=255)     tgAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGc                                                                                                                        >  1:429117/1‑140 (MQ=255)       aaGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTg                                                                                                                      >  1:380643/1‑140 (MQ=255)          gTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGaa                                                                                                                   >  2:321585/1‑140 (MQ=255)             aaaaCGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAAtc                                                                                                                >  1:461952/1‑140 (MQ=255)                 cGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATctcct                                                                                                            >  2:109355/1‑140 (MQ=255)                    aCTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGt                                                                                                         <  1:484434/140‑1 (MQ=255)                       gCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTg                                                                                                      <  1:321585/140‑1 (MQ=255)                                  tGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAg                                                                                           <  2:380643/140‑1 (MQ=255)                                  tGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAg                                                                                           <  2:329690/140‑1 (MQ=255)                                  tGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAg                                                                                           <  1:425109/140‑1 (MQ=255)                                  tGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAg                                                                                           <  2:257400/140‑1 (MQ=255)                                    aCGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCa                                                                                         <  2:148490/140‑1 (MQ=255)                                     cGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCaa                                                                                        >  1:413018/1‑140 (MQ=255)                                              aCGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAct                                                                               >  1:212212/1‑140 (MQ=255)                                              aCGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCAct                                                                               >  1:516981/1‑140 (MQ=255)                                                                            ttGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAagcagc                                                 >  2:160322/1‑140 (MQ=255)                                                                                  tGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTc                                           >  2:443136/1‑140 (MQ=255)                                                                                   gAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCg                                          <  1:160322/140‑1 (MQ=255)                                                                                         tggtgATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGc                                    >  2:287437/1‑140 (MQ=255)                                                                                         tggtgATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGc                                    >  2:537269/1‑140 (MQ=255)                                                                                         tggtgATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGc                                    >  2:469087/1‑140 (MQ=255)                                                                                           gtgATTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGcac                                  <  1:109355/140‑1 (MQ=255)                                                                                              aTTACGCTGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACAcc                               >  2:86335/1‑140 (MQ=255)                                                                                                     tGTCAACGTCAACTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTg                        <  2:461952/140‑1 (MQ=255)                                                                                                       tcaacgtcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGgc                      >  2:63368/1‑140 (MQ=255)                                                                                                       tcaacgtcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGgc                      >  2:391858/1‑140 (MQ=255)                                                                                                        caacgtcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGgcg                     >  1:297508/1‑140 (MQ=255)                                                                                                        caacgtcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGgcg                     >  2:531909/1‑140 (MQ=255)                                                                                                        caacgtcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGgcg                     <  1:531909/140‑1 (MQ=255)                                                                                                             tcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCgcggcg                <  2:413018/140‑1 (MQ=255)                                                                                                             tcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCgcggcg                <  2:516981/140‑1 (MQ=255)                                                                                                             tcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCgcggcg                <  2:212212/140‑1 (MQ=255)                                                                                                             tcaacTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTG‑CACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGt               >  1:202005/1‑140 (MQ=255)                                                                                                                 cTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACt            >  1:243840/1‑140 (MQ=255)                                                                                                                 cTACAACGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACt            >  2:364344/1‑140 (MQ=255)                                                                                                                     aaCGTGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCAc        >  1:257901/1‑140 (MQ=255)                                                                                                                         tGCAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCt    >  2:182560/1‑140 (MQ=255)                                                                                                                           cAGAGCGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGa  <  1:443136/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                        TGGCTGAAGGTCAAAACGAACTGCAGGTGCCGATGACGTATACCGACGCGGCAGGCAACACGTTTACCAAAACGTTTGTCCTGAAACGTGGTGATTACGCTGTCAACGTCAACTACAACGTGCAGAACGCTGGCGAGAAACCGCTGGAAATCTCCTCGTTTGGTCAGTTGAAGCAATCCATCACTCTGCCACCGCATCTCGATACCGGAAGCAGCAACTTCGCACTGCACACCTTCCGTGGCGCGGCGTACTCCACGCCTGA  >  NC_000913/3885494‑3885755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑