Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,902,375	T→C	T443A (ACA→GCA)	bglB ←	6‑phospho‑beta‑glucosidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,902,375	0	T	C	100.0%	112.1 / NA	32	T443A (ACA→GCA)	bglB	6‑phospho‑beta‑glucosidase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/15);  total (17/15)

CAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCATAATATCCACA  >  NC_000913/3902238‑3902505                                                                                                                                          |                                                                                                                                   cAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTc                                                                                                                                  >  2:434613/1‑140 (MQ=255)     gaaagaaTTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTtgt                                                                                                                              <  1:434613/140‑1 (MQ=255)          aaTTAGCGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGc                                                                                                                         <  1:375892/140‑1 (MQ=255)              aaCGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGcttcc                                                                                                                     >  2:533064/1‑140 (MQ=255)                    tcgcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGcc                                                                                                               >  2:279766/1‑140 (MQ=255)                      gcatcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCAt                                                                                                             <  1:533064/140‑1 (MQ=255)                        atcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTa                                                                                                           <  2:158629/140‑1 (MQ=255)                         tcCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTat                                                                                                          <  2:16619/140‑1 (MQ=255)                            aGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTatcat                                                                                                       <  2:391564/140‑1 (MQ=255)                                   tCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATc                                                                                                <  2:356002/140‑1 (MQ=255)                                      gAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                                                                                             <  2:43002/140‑1 (MQ=255)                                       aaTGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTACTGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcaca                                                                                            <  2:507810/140‑1 (MQ=255)                                            gacgaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                                                                                             <  1:349512/134‑1 (MQ=255)                                            gacgaTAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCcac                                                                                             >  2:349512/1‑134 (MQ=255)                                                     tAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTa                                                                              <  1:304053/140‑1 (MQ=255)                                                                                      aGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGAc                                             >  2:429396/1‑140 (MQ=255)                                                                                                       cACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGccccc                            <  1:279766/140‑1 (MQ=255)                                                                                                       cACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGccccc                            <  1:429396/140‑1 (MQ=255)                                                                                                             tGCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTgg                      >  2:30047/1‑140 (MQ=255)                                                                                                              gCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAACCAAt                                    <  1:523581/125‑1 (MQ=255)                                                                                                              gCATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAACCAAt                                    >  2:523581/1‑125 (MQ=255)                                                                                                               cATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGgtg                    >  2:427336/1‑140 (MQ=255)                                                                                                               cATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGgtg                    >  1:291375/1‑140 (MQ=255)                                                                                                               cATACCATCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGgtg                    >  2:546097/1‑140 (MQ=255)                                                                                                                     aTCCGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTAcccc              >  1:366677/1‑140 (MQ=255)                                                                                                                       ccGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCat            >  2:132964/1‑140 (MQ=255)                                                                                                                       ccGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCat            >  1:92432/1‑140 (MQ=255)                                                                                                                       ccGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCat            >  1:459734/1‑140 (MQ=255)                                                                                                                       ccGAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCat            >  1:119454/1‑140 (MQ=255)                                                                                                                         gAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGccccc                            >  1:466706/1‑122 (MQ=255)                                                                                                                         gAAGCTTTTCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGccccc                            <  2:466706/122‑1 (MQ=255)                                                                                                                                 tCTTGCGTGCTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCATAATATCcaca  >  1:537897/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   CAAAGAAAGAATTAACGCATCGCATCCAGACTGTTCTGAATGCGACGATAATTAAGGTGCTTTAATGGTTATTTTTTTTAATGACAGCCCCCGCGTCTTGATCACCTCTGCATACCATCCGAAGCTTTTCTTGCGTGTTCTTGTGAGGCTTCCTTCGCCATTATCATCACGATCCACATAAATAAAGCCGTAGCGCTTAGACATTTGTGAATGAGATGCACTGACTAAATCAATTGGCCCCCAACTGGTGTACCCCATAATATCCACA  >  NC_000913/3902238‑3902505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑