Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,197,163	A→G	Q87Q (CAA→CAG)	nudC →	NADH pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,197,163	0	A	G	100.0%	119.6 / NA	34	Q87Q (CAA→CAG)	nudC	NADH pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

GCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCC  >  NC_000913/4197027‑4197286                                                                                                                                         |                                                                                                                            gcgcACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGTGTACAGCTg                                                                                                                          >  1:132024/1‑140 (MQ=255)     aCTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCg                                                                                                                       <  1:160943/139‑1 (MQ=255)     aCTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCg                                                                                                                       >  2:160943/1‑139 (MQ=255)      cTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGa                                                                                                                     <  1:67457/140‑1 (MQ=255)               ggCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCg                                                                                                            >  2:356440/1‑140 (MQ=255)                 cGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGAt                                                                                                          >  1:27503/1‑140 (MQ=255)                 cGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGAt                                                                                                          >  2:303590/1‑140 (MQ=255)                 cGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGAt                                                                                                          >  1:105702/1‑140 (MQ=255)                 cGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGAt                                                                                                          >  1:511487/1‑140 (MQ=255)                      ggCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCAt                                                                                                     <  2:27503/140‑1 (MQ=255)                           ggggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATa                                                                                                >  1:490684/1‑140 (MQ=255)                            gggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATAc                                                                                               <  2:75879/140‑1 (MQ=255)                            gggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATAc                                                                                               <  2:22149/140‑1 (MQ=255)                             ggAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGTGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACt                                                                                              <  2:132024/140‑1 (MQ=255)                              gAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACtg                                                                                             <  1:273585/140‑1 (MQ=255)                                             gTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCa                                                                              <  1:21322/140‑1 (MQ=255)                                             gTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCa                                                                              <  2:105702/140‑1 (MQ=255)                                              tACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCAt                                                                             >  1:212312/1‑140 (MQ=255)                                                   cagcagCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAAt                                                                        >  1:36816/1‑140 (MQ=255)                                                   cagcagCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAAt                                                                        >  2:390333/1‑140 (MQ=255)                                                    agcagcGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATg                                                                       <  1:455095/140‑1 (MQ=255)                                                       agcGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTAt                                                                    >  2:317360/1‑140 (MQ=255)                                                            cgTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAg                                                               <  1:303590/140‑1 (MQ=255)                                                            cgTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAg                                                               <  2:212312/140‑1 (MQ=255)                                                                            cGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCg                                               >  1:341482/1‑140 (MQ=255)                                                                              gTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGAt                                             >  2:435768/1‑140 (MQ=255)                                                                                       gTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAg                                    >  2:296406/1‑140 (MQ=255)                                                                                       gTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAg                                    <  2:490684/140‑1 (MQ=255)                                                                                                 tcGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGt                          <  1:356440/140‑1 (MQ=255)                                                                                                                     cTGGCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAAt      <  2:36816/140‑1 (MQ=255)                                                                                                                       ggCCGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCg    <  1:296406/140‑1 (MQ=255)                                                                                                                         ccGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGcc  <  2:184612/140‑1 (MQ=255)                                                                                                                         ccGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGcc  >  1:184612/1‑140 (MQ=255)                                                                                                                         ccGGACGAGGCGTACAGCTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGcc  >  1:368708/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                            GCGCACTACAAATCGGCGAATGGCAGGGGGAACCTGTTTGGTTAGTACAACAGCAGCGGCGTCACGATATGGGGTCGGTACGTCAGGTCATTGATCTCGATGTTGGGCTGTTTCAACTGGCCGGACGAGGCGTACAACTGGCGGAGTTTTACCGATCGCATAAATACTGTGGTTACTGCGGGCATGAAATGTATCCGAGCAAAACCGAATGGGCGATGCTGTGCAGCCATTGCCGTGAGCGTTACTACCCGCAAATCGCC  >  NC_000913/4197027‑4197286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑