Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,335,130	A→G	V154A (GTT→GCT)	eptA ←	phosphoethanolamine transferase EptA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,335,130	0	A	G	100.0%	169.3 / NA	48	V154A (GTT→GCT)	eptA	phosphoethanolamine transferase EptA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/20);  total (28/20)

TGTTAGAGGGGCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAACACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGTATCAA  >  NC_000913/4334999‑4335265                                                                                                                                    |                                                                                                                                        tGTTAGAGGGGCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTgcgc                                                                                                                                 >  1:101848/1‑140 (MQ=255) tGTTAGAGGGGCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTgcgc                                                                                                                                 >  1:67688/1‑140 (MQ=255)          ggCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAg                                                                                                                        >  2:194347/1‑140 (MQ=255)           gCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAgg                                                                                                                       >  1:68825/1‑140 (MQ=255)           gCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAgg                                                                                                                       >  2:443093/1‑140 (MQ=255)                     ttCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGttt                                                                                                             >  1:334989/1‑140 (MQ=255)                     ttCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGttt                                                                                                             <  2:101848/140‑1 (MQ=255)                     ttCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGttt                                                                                                             <  2:67688/140‑1 (MQ=255)                                             aacaacGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAg                                                                                     <  2:193801/140‑1 (MQ=255)                                                  cGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCgcag                                                                                >  2:326950/1‑140 (MQ=255)                                                         tAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAAt                                                                                        >  2:19579/1‑125 (MQ=255)                                                         tAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAAt                                                                                        <  1:19579/125‑1 (MQ=255)                                                               ttATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCt                                                                   <  1:240162/140‑1 (MQ=255)                                                                tataAAACAGTGCGGCGTCCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTg                                                                  >  2:343955/1‑140 (MQ=255)                                                                      aCAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATccc                                                            >  1:301164/1‑140 (MQ=255)                                                                      aCAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATccc                                                            >  1:365471/1‑140 (MQ=255)                                                                        aGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAg                                                          >  1:136891/1‑140 (MQ=255)                                                                          tGCGGCGTCCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCg                                                        <  1:343955/140‑1 (MQ=255)                                                                          tGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCg                                                        >  2:305674/1‑140 (MQ=255)                                                                          tGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCg                                                        >  1:123898/1‑140 (MQ=255)                                                                          tGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCg                                                        >  2:21458/1‑140 (MQ=255)                                                                          tGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCg                                                        >  1:543548/1‑140 (MQ=255)                                                                          tGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCg                                                        >  1:375274/1‑140 (MQ=255)                                                                               cgACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTtaat                                                   <  1:357831/140‑1 (MQ=255)                                                                               cgACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTtaat                                                   >  2:257778/1‑140 (MQ=255)                                                                                    aGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACAt                                              >  1:39531/1‑140 (MQ=255)                                                                                               tagtaCAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGc                                          <  2:147825/133‑1 (MQ=255)                                                                                               tagtaCAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGc                                          >  1:147825/1‑133 (MQ=255)                                                                                                agtaCAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGtcat                                  >  2:41628/1‑140 (MQ=255)                                                                                                    cAGAAACCAGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAgc                              >  1:378824/1‑140 (MQ=255)                                                                                                            aGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACt                      >  1:271863/1‑140 (MQ=255)                                                                                                            aGAATATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACt                      >  2:384582/1‑140 (MQ=255)                                                                                                              aaTATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACttt                    >  2:302986/1‑140 (MQ=255)                                                                                                              aaTATTGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACttt                    >  2:384965/1‑140 (MQ=255)                                                                                                                   tGGCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGcc               <  2:543548/140‑1 (MQ=255)                                                                                                                    ggCTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCg              <  2:334989/140‑1 (MQ=255)                                                                                                                      cTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGa            <  2:375274/140‑1 (MQ=255)                                                                                                                      cTCCACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGa            <  2:365471/140‑1 (MQ=255)                                                                                                                        ccACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGt          >  1:128407/1‑140 (MQ=255)                                                                                                                         cACGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTg         <  1:41628/140‑1 (MQ=255)                                                                                                                           cGGAAAAGAGGACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGt       <  2:70380/140‑1 (MQ=255)                                                                                                                           cGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGc                <  2:512826/131‑1 (MQ=255)                                                                                                                           cGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGc                >  1:512826/1‑131 (MQ=255)                                                                                                                           cGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGt       <  1:302986/140‑1 (MQ=255)                                                                                                                           cGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGt       <  2:219006/140‑1 (MQ=255)                                                                                                                           cGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGt       <  1:384582/140‑1 (MQ=255)                                                                                                                           cGGAAAAGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGt       <  1:384965/140‑1 (MQ=255)                                                                                                                                aaGAGCACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGTATCaa  <  1:194347/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        TGTTAGAGGGGCTTAAGGATTTCACCAGCTCTTTGTTATTGCGGAACAACGAGGCGTAGTCTTTATAAAACAGTGCGGCGACCAGCAAAATCAGTAGTACAGAAACCAGAATATTGGCTCCACGGAAAAGAACACTGCGCAGACGCGAGGTGGCAGGTTTGATTTTTATCCAGCAGGCAATCAGCGCAGCAAGCACGCCGCTGAATCCCAGCGTTAATAACATTTGCGGTGTCATCAGCGCATAACTTTCTGCCGGAGTGGTATCAA  >  NC_000913/4334999‑4335265

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑