Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,378,361	A→G	F195S (TTC→TCC)	ampC ←	beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,378,361	0	A	G	100.0%	125.7 / NA	36	F195S (TTC→TCC)	ampC	beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/17);  total (19/17)

CTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGTTTTGATAGA  >  NC_000913/4378225‑4378496                                                                                                                                         |                                                                                                                                        cTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGgaga                                                                                                                                      <  2:367772/140‑1 (MQ=255)   tCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGaca                                                                                                                                    <  2:468594/140‑1 (MQ=255)           tAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTg                                                                                                                            >  1:522388/1‑140 (MQ=255)                  cAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGcc                                                                                                                     >  2:84679/1‑140 (MQ=255)                        cTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCa                                                                                                               >  1:499967/1‑140 (MQ=255)                          aaCGCCCCAGGTGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCaaa                                                                                                             >  2:229349/1‑140 (MQ=255)                             gCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACt                                                                                                          <  1:259086/140‑1 (MQ=255)                                   ggCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAAcc                                                                                                    >  2:388093/1‑140 (MQ=255)                                      gAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGa                                                                                                 <  2:515795/140‑1 (MQ=255)                                      gAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGa                                                                                                 <  2:237763/140‑1 (MQ=255)                                              cACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTca                                                                                         >  2:313131/1‑140 (MQ=255)                                                       aCCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTg                                                                                >  1:304510/1‑140 (MQ=255)                                                             gcgATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGa                                                                          >  1:282536/1‑140 (MQ=255)                                                                   tCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAAc                                                                    <  1:152585/140‑1 (MQ=255)                                                                          gCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACt                                                             >  2:369306/1‑140 (MQ=255)                                                                           cGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTg                                                            >  2:91464/1‑140 (MQ=255)                                                                              aaTTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAg                                                         >  2:278395/1‑140 (MQ=255)                                                                               aTTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGt                                                        >  1:72482/1‑140 (MQ=255)                                                                               aTTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGt                                                        >  2:433859/1‑140 (MQ=255)                                                                               aTTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGt                                                        <  2:282536/140‑1 (MQ=255)                                                                               aTTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGt                                                        <  2:42248/140‑1 (MQ=255)                                                                                    tttcttCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGTATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCa                                                   >  1:519015/1‑140 (MQ=255)                                                                                         tctGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAg                                              <  1:91464/140‑1 (MQ=255)                                                                                                 cggTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTtgtg                                      <  2:346923/140‑1 (MQ=255)                                                                                                      cATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCt                                 <  1:388093/140‑1 (MQ=255)                                                                                                      cATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCt                                 <  1:433859/140‑1 (MQ=255)                                                                                                       aTTAATCCACGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTg                                <  2:137943/140‑1 (MQ=255)                                                                                                                cGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATg                       >  1:254416/1‑140 (MQ=255)                                                                                                                cGTATGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTAAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATg                       >  2:42731/1‑140 (MQ=255)                                                                                                                   aTGGTTGAGTTTGAGTGGCTGGGAGACACGAGTTTGCATCGCCTGCTAAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAg                    <  1:42731/140‑1 (MQ=255)                                                                                                                       ttgagtttgagtGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTg                <  1:129893/140‑1 (MQ=255)                                                                                                                          agtttgagtGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCa             >  2:493381/1‑140 (MQ=255)                                                                                                                            tttgagtGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGt           <  1:319415/140‑1 (MQ=255)                                                                                                                               gagtGGCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGtttt        >  1:481527/1‑140 (MQ=255)                                                                                                                                    gCTGGGAGACACGAGTTTGTATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGTTTTGATAg   <  2:519015/140‑1 (MQ=255)                                                                                                                                    gCTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGTTTTGATAg   <  2:499967/140‑1 (MQ=255)                                                                                                                                     cTGGGAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGTTTTGATAGa  >  2:374409/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTATGGTTGAGTTTGAGTGGCTGGAAGACACGAGTTTGCATCGCCTGCTCAAAACTCAAACCAGACGGCTTCACAGCCAGTGCGCCGAACAAACCGATACTGGAGTTGGCATACAGACGTTGTGTTCCTGGAGCCCATGCAGGCTGCCAGTTTTGATAGA  >  NC_000913/4378225‑4378496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑