| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,595,493 | C→T | G120D (GGT→GAT) | lgoR ← | putative DNA‑binding transcriptional regulator LgoR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,595,493 | 0 | C | T | 95.2% | 62.5 / ‑4.8 | 21 | G120D (GGT→GAT) | lgoR | putative DNA‑binding transcriptional regulator LgoR |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (11/9); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCG > NC_000913/4595375‑4595627 | ctgctCGACGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCa > 2:59630/1‑140 (MQ=255) ggCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAg > 2:147224/1‑140 (MQ=255) cGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGaa < 2:280093/140‑1 (MQ=255) ttGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGttt > 1:348005/1‑140 (MQ=255) tGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTc > 1:131345/1‑140 (MQ=255) ttGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGc < 1:223598/140‑1 (MQ=255) gCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgc < 2:224105/140‑1 (MQ=255) cTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGgcg < 2:348005/140‑1 (MQ=255) cTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTggtg < 1:409615/140‑3 (MQ=255) ccGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTat > 1:135084/1‑140 (MQ=255) cgcgCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTatc > 2:242962/1‑140 (MQ=255) gAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGcc < 1:242962/140‑1 (MQ=255) taACGGCCGCATTTTAAAAGATATTCTCGTACAACAACCGGGCTGACATCCGCGGCCGGAGCCAGGTGCAGTTCAGAAAACGTTTCCCCTGGGCGAAACTGGCGCTGGCTTATCATCGAGAAATCAGCCTGCTCAAacac > 2:522130/1‑140 (MQ=255) ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACtttgtt > 2:265554/1‑140 (MQ=255) ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACttt < 2:101448/137‑1 (MQ=255) ccGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACttt > 1:101448/1‑137 (MQ=255) tatTCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGTGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGa < 1:59630/140‑1 (MQ=255) ttCTCTTACTACAACCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAg < 2:135084/140‑1 (MQ=255) aCCGGGCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGc > 1:388202/1‑140 (MQ=255) ggCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa > 2:135762/1‑140 (MQ=255) ggCTGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGaaa > 2:288473/1‑140 (MQ=255) tGACATCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCg < 1:288473/140‑1 (MQ=255) | CTGCTCGGCGTAGGATTGATCGAATTGCTTCATGCTCCACTGGCCGCGCTTTTCGCTGTGAATAAGATTATAACGGCCGAATTTTAAAAGATATTCTCTTACTACAACCGGGCTGACACCCGCGGCCCGAGCCAGTTGCAGTTCAGAAAACGTTTCCCCTGGGCGTAACTGGCGCTGGTTTATCATCGTGAAAAAAGCCTGCTCAAACACTTTGTTTTGTTCACTCATTGAAGCGGTAGTACAGGCGAAACCG > NC_000913/4595375‑4595627 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |