Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	706,847	T→C	V252A (GTT→GCT)	glnS →	glutamine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	706,847	0	T	C	100.0%	211.8 / NA	60	V252A (GTT→GCT)	glnS	glutamine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/29);  total (31/29)

GTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCC  >  NC_000913/706710‑706977                                                                                                                                          |                                                                                                                                   gtgCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTc                                                                                                                                  >  2:126940/1‑140 (MQ=255) gtgCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTc                                                                                                                                  >  1:323414/1‑140 (MQ=255)       cTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCcgc                                                                                                                            <  1:207279/140‑1 (MQ=255)         aCCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCgcgc                                                                                                                          >  2:116902/1‑140 (MQ=255)         aCCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCgcgc                                                                                                                          <  2:488476/140‑1 (MQ=255)         aCCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCgcgc                                                                                                                          <  1:329410/140‑1 (MQ=255)            cGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGATAACATCACGATTCCTGCTCACCCGCGCCAg                                                                                                                       >  2:128187/1‑140 (MQ=255)             gATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGt                                                                                                                      <  2:206197/140‑1 (MQ=255)               tGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTAt                                                                                                                    >  2:384437/1‑140 (MQ=255)                         cACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTcgc                                                                                                          >  1:188174/1‑140 (MQ=255)                         cACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTcgc                                                                                                          >  2:142640/1‑140 (MQ=255)                          aCCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTcgcg                                                                                                         >  2:311075/1‑140 (MQ=255)                           cccACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCgcgc                                                                                                        >  1:185314/1‑140 (MQ=255)                            ccACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGcc                                                                                                       <  2:185314/140‑1 (MQ=255)                               cTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGa                                                                                                    >  2:43861/1‑140 (MQ=255)                                 gCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAAt                                                                                                  <  2:323414/140‑1 (MQ=255)                                 gCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAAt                                                                                                  <  1:4310/140‑1 (MQ=255)                                  cATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTcg                                                                                                           <  1:263981/130‑1 (MQ=255)                                  cATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTcg                                                                                                           >  2:263981/1‑130 (MQ=255)                                   aTCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCt                                                                                                <  1:126940/140‑1 (MQ=255)                                    tCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTg                                                                                               >  1:265487/1‑140 (MQ=255)                                       gCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa                                                                                            >  2:43348/1‑140 (MQ=255)                                       gCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGaa                                                                                            <  2:179472/140‑1 (MQ=255)                                        cGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGa                                                                                                    <  2:161888/131‑1 (MQ=255)                                        cGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGa                                                                                                    >  1:161888/1‑131 (MQ=255)                                          aTGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACTACCGTCGTCCGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATac                                                                                         <  2:486412/140‑1 (MQ=255)                                          aTGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATac                                                                                         <  2:354133/140‑1 (MQ=255)                                          aTGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATac                                                                                         >  2:202032/1‑140 (MQ=255)                                              ccTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCg                                                                                     <  1:392491/140‑1 (MQ=255)                                                       tATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGa                                                                                             >  1:77950/1‑123 (MQ=255)                                                       tATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGa                                                                                             <  2:77950/123‑1 (MQ=255)                                                          tACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGc                                                                         <  2:265487/140‑1 (MQ=255)                                                                ctctctGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGt                                                                   >  2:36002/1‑140 (MQ=255)                                                                   tctGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGa                                                                >  1:455964/1‑140 (MQ=255)                                                                          aCGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACctgct                                                         <  2:197624/140‑1 (MQ=255)                                                                           cGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACctgctg                                                        >  1:223736/1‑140 (MQ=255)                                                                             cTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGt                                                      >  1:476743/1‑140 (MQ=255)                                                                             cTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGt                                                      >  1:237794/1‑140 (MQ=255)                                                                               tGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGa                                                    >  2:289550/1‑140 (MQ=255)                                                                               tGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGa                                                    >  1:412404/1‑140 (MQ=255)                                                                               tGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGa                                                    <  1:207975/140‑1 (MQ=255)                                                                                 aGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGAcc                                                  >  2:91324/1‑140 (MQ=255)                                                                                 aGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGAcc                                                  >  1:144164/1‑140 (MQ=255)                                                                                        ggACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGc                                           <  1:202032/140‑1 (MQ=255)                                                                                             aCCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGtt                                      >  2:49781/1‑140 (MQ=255)                                                                                              ccGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTg                                     <  1:43348/140‑1 (MQ=255)                                                                                                        tACGACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGa                           >  2:280144/1‑140 (MQ=255)                                                                                                           gACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGa                        <  1:43861/140‑1 (MQ=255)                                                                                                           gACTGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGa                        <  1:36002/140‑1 (MQ=255)                                                                                                              tGGGTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGAccc                     >  1:340321/1‑140 (MQ=255)                                                                                                                ggTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCcgc                   <  1:49781/140‑1 (MQ=255)                                                                                                                ggTACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCcgc                   <  1:27898/140‑1 (MQ=255)                                                                                                                  tACTGGACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGt                 >  2:153684/1‑140 (MQ=255)                                                                                                                      ggACAACATCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGc             <  1:384437/140‑1 (MQ=255)                                                                                                                        acaacaTCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCg           <  2:188174/140‑1 (MQ=255)                                                                                                                          aacaTCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc         <  1:311075/140‑1 (MQ=255)                                                                                                                          aacaTCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc         <  1:128187/140‑1 (MQ=255)                                                                                                                            caTCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc         <  1:192811/138‑1 (MQ=255)                                                                                                                            caTCACGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGAc         >  2:192811/1‑138 (MQ=255)                                                                                                                                 cGATTCCTGCTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTcc  >  1:413265/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                   GTGCATCTACCCGATGTACGACTTCACCCACTGCATCAGCGATGCCCTGGAAGGTATTACGCACTCTCTGTGTACGCTTGAGTTCCAGGACAACCGTCGTCTGTACGACTGGGTACTGGACAACATCACGATTCCTGTTCACCCGCGCCAGTATGAGTTCTCGCGCCTGAATCTGGAATACACCGTGATGTCCAAGCGTAAGTTGAACCTGCTGGTGACCGACAAGCACGTTGAAGGCTGGGATGACCCGCGTATGCCGACCATTTCC  >  NC_000913/706710‑706977

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑