Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	777,361	T→C	Y340Y (TAT→TAC)	tolA →	Tol‑Pal system protein TolA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	777,361	0	T	C	100.0%	63.8 / NA	19	Y340Y (TAT→TAC)	tolA	Tol‑Pal system protein TolA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/7);  total (12/7)

ATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGAT  >  NC_000913/777228‑777493                                                                                                                                      |                                                                                                                                     atatTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCggg                                                                                                                                <  1:397256/140‑1 (MQ=255)          gTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGAGCCGATATCAATAACTACGCCGGGCAGATTaaa                                                                                                                       >  1:436976/1‑140 (MQ=255)          gTGAGCTAAGCTCTGGAAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTaaa                                                                                                                       >  1:464637/1‑140 (MQ=255)                  aGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTAt                                                                                                               >  2:47356/1‑140 (MQ=255)                         gTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAaagt                                                                                                        <  1:6109/140‑1 (MQ=255)                              aaTGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCG‑‑ATCAATGACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCt                                                                                                 >  2:508466/1‑140 (MQ=255)                                   aCCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATg                                                                                              >  1:321562/1‑140 (MQ=255)                                    ccGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGtt                                                                                                   >  1:480774/1‑134 (MQ=255)                                    ccGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACAACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGtt                                                                                                   <  2:480774/134‑1 (MQ=255)                                     cGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGAc                                                                                            >  2:372767/1‑140 (MQ=255)                                                         aaaGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGCGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGGaa                                                                        <  2:464637/140‑1 (MQ=255)                                                                             tGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATaa                                                    <  1:8576/140‑1 (MQ=255)                                                                                   gAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTgg                                              <  1:372767/140‑1 (MQ=255)                                                                                        gTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCAccc                                         >  1:245107/1‑140 (MQ=255)                                                                                                aaaaaCAATGGCGCATCAGGGGCCGATATCAGTAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTAt                                 >  2:231720/1‑140 (MQ=255)                                                                                                   aaCAATGGCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGtt                              >  1:382180/1‑140 (MQ=255)                                                                                                     cAATGGCGCATCAGGGGCCGATATCAGTAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTAc                            <  1:231720/140‑1 (MQ=255)                                                                                                         ggCGCATCAGGGGCCGATATCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGa                        >  1:431026/1‑140 (MQ=255)                                                                                                                           atCAATAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTgg      <  1:47356/140‑1 (MQ=255)                                                                                                                               aTAACTACGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGAt  >  1:303150/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                     ATATTTTCGGTGAGCTAAGCTCTGGTAAGAATGCACCGAAAACGGGGGGAGGGGCGAAAGGGAACAATGCTTCGCCTGCCGGGAGTGGTAATACTAAAAACAATGGCGCATCAGGGGCCGATATCAATAACTATGCCGGGCAGATTAAATCTGCTATCGAAAGTAAGTTCTATGACGCATCGTCCTATGCAGGCAAAACCTGTACGCTGCGCATAAAACTGGCACCCGATGGTATGTTACTGGATATCAAACCTGAAGGTGGCGAT  >  NC_000913/777228‑777493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑