Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	853,778	C→T	T44T (ACC→ACT)	ybiR →	putative transporter YbiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	853,778	0	C	T	100.0%	51.8 / NA	16	T44T (ACC→ACT)	ybiR	putative transporter YbiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/7);  total (9/7)

GCCTTTTTTACGCACGCTGCAAGGCGATCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACCATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGCGCCGCTTTGCTACGGAGCGTCGGCTGGCGATGTTTATGGTG  >  NC_000913/853655‑853904                                                                                                                            |                                                                                                                               gcctTTTTTACGCACGCTGCAAGGCGATCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCg                                                                                                                >  1:292442/1‑140 (MQ=255)  cctTTTTTACGCACGCTGCAAGGCGATCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCgg                                                                                                               >  2:52337/1‑140 (MQ=255)            gcacgcTGCAAGGCGATCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGAtgctg                                                                                                     >  1:484096/1‑140 (MQ=255)                gcTGCAAGGCGATCGTTTTTTTAAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGa                                                                                                 >  1:394745/1‑140 (MQ=255)                          gATCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGgtgt                                                                                       >  1:344264/1‑140 (MQ=255)                            tCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTgg                                                                                     >  1:330317/1‑140 (MQ=255)                              gTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAg                                                                                   <  2:292442/140‑1 (MQ=255)                                         ttattaATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTa                                                                        <  2:484227/140‑1 (MQ=255)                                                        ggTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTggg                                                         <  2:330317/140‑1 (MQ=255)                                                                     gCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTg                                            <  2:484096/140‑1 (MQ=255)                                                                        ttttCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTgcgc                                         <  2:406382/140‑1 (MQ=255)                                                                               gCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGcgccg                                       >  1:33088/1‑135 (MQ=255)                                                                               gCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGcgccg                                       <  2:33088/135‑1 (MQ=255)                                                                                                         ctgctATCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGCGCCGCTTTGCTACGGAGCGTCGGCTGGCGATGttt        <  2:344264/140‑1 (MQ=255)                                                                                                              aTCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGCGCCGCTTTGCTACGGAGCGTCGGCTGGCGATGTTTATGGt   >  1:297042/1‑140 (MQ=255)                                                                                                               tCGACTGGCACACTATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGCGCCGCTTTGCTACGGAGCGTCGGCTGGCGATGTTTATGGTg  >  2:372061/1‑140 (MQ=255)                                                                                                                            |                                                                                                                               GCCTTTTTTACGCACGCTGCAAGGCGATCGTTTTTTTCAGTTATTAATTCTTGTTGGTATCGGATTAAGCTTTTTCGTGCCCTTTGCACCGAAATCCTGGCCTGCTGCTATCGACTGGCACACCATCATCACCTTAAGCGGCCTGATGCTGCTGACCAAAGGTGTGGAGTTAAGCGGTTATTTTGATGTGCTGGGGCGCAAAATGGTGCGCCGCTTTGCTACGGAGCGTCGGCTGGCGATGTTTATGGTG  >  NC_000913/853655‑853904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑