Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	945,505	A→G	V18A (GTT→GCT)	ycaC ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	945,505	0	A	G	100.0%	89.4 / NA	26	V18A (GTT→GCT)	ycaC	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/11);  total (15/11)

TTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACGGGAAATA  >  NC_000913/945374‑945639                                                                                                                                    |                                                                                                                                       tttCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCaaa                                                                                                                                >  1:420098/1‑140 (MQ=255)                          tGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGAc                                                                                                       <  1:396897/140‑1 (MQ=255)                           ggTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACg                                                                                                      >  2:12119/1‑140 (MQ=255)                                           tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc                                                                                      <  1:12119/140‑1 (MQ=255)                                           tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc                                                                                      >  1:202786/1‑140 (MQ=255)                                           tAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTc                                                                                      >  1:63954/1‑140 (MQ=255)                                                  gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCctct                                                                               >  2:445190/1‑140 (MQ=255)                                                  gTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCctct                                                                               >  2:149930/1‑140 (MQ=255)                                                   tCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCtctt                                                                              <  2:202786/140‑1 (MQ=255)                                                     aCCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCtcttct                                                                            <  2:305576/140‑1 (MQ=255)                                                           cgcCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGAGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTc                                                                      >  2:317529/1‑140 (MQ=255)                                                            gcCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCg                                                                     >  1:91072/1‑140 (MQ=255)                                                                gCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGagag                                                                 <  1:149930/140‑1 (MQ=255)                                                                     ttATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGt                                                            >  2:236237/1‑140 (MQ=255)                                                                                  tATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGaaa                                               >  1:451197/1‑140 (MQ=255)                                                                                   aTCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAAt                                              >  1:346017/1‑140 (MQ=255)                                                                                      gggTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGa                                           <  1:534303/140‑1 (MQ=255)                                                                                                          gggAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGctc                       >  2:356567/1‑140 (MQ=255)                                                                                                            gAAAGTAAACCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTg                     <  2:346017/140‑1 (MQ=255)                                                                                                                  aaaCCAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCa               <  1:236237/140‑1 (MQ=255)                                                                                                                     ccAGCCTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAg            >  2:70034/1‑140 (MQ=255)                                                                                                                     ccAGCCTGGAGATCAGCAAGCAAAACGGCAGTATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAg            <  1:317529/140‑1 (MQ=255)                                                                                                                         ccTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACgg        <  2:451197/140‑1 (MQ=255)                                                                                                                         ccTGGTGATCAGCAAGCAAAACGGCAGCATCA‑TTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACggg       >  1:110703/1‑140 (MQ=255)                                                                                                                          cTGGTGATCAGCAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACggg       >  1:321146/1‑140 (MQ=255)                                                                                                                              tGATCAGCAAGCAAAACGGCAGCATCA‑TTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACGGGAAata  <  2:110703/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                       TTTCAAAACTGGTGGTAAGAATAGTTGGTAAGTTGAAATACTTAGCCAGGTCACCCAGCGCCAGCACGTTATTTTTAAACTTATCGGGTTCGATATCCCGTACAAGGGAAAGTAAACCAGCCTGGTGATCAACAAGCAAAACGGCAGCATCATTTTTATCAAGACGAACATACGGTTTGGTCATCCTCTTCTCCTTTCGAGAGTCAGTGGTACGTCTGAAATCGATCAGACGCGATGCATTGCTCTGAAAGCATAGACGGGAAATA  >  NC_000913/945374‑945639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑