Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	218,588	T→G	N63T (AAC→ACC)	proS ←	proline‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	218,588	0	T	G	100.0%	64.1 / NA	19	N63T (AAC→ACC)	proS	proline‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/9);  total (10/9)

GAACGGACGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGTTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGCTTGCGGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGC  >  NC_000913/218464‑218724                                                                                                                             |                                                                                                                                         gAACGGACGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTcac                                                                                                                           >  1:36833/1‑140 (MQ=255)  aaCGGACGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTcacg                                                                                                                          <  1:171920/140‑1 (MQ=255)       aCGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGa                                                                                                                     >  2:56753/1‑140 (MQ=255)        cGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGAt                                                                                                                    >  1:383226/1‑140 (MQ=255)                              gcagcaGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAAcg                                                                                              >  1:172510/1‑140 (MQ=255)                                agcagTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAAcgcg                                                                                            <  2:36833/140‑1 (MQ=255)                                           aCCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCg                                                                                 <  2:172510/140‑1 (MQ=255)                                               tACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAg                                                                             >  2:310961/1‑140 (MQ=255)                                                aCTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGc                                                                            <  2:34665/140‑1 (MQ=255)                                                aCTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGc                                                                            <  2:383226/140‑1 (MQ=255)                                                 cTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGcc                                                                           <  2:417722/140‑1 (MQ=255)                                                       ccAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGtat                                                                     >  1:321777/1‑140 (MQ=255)                                                              ccACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAAccc                                                              >  2:425235/1‑140 (MQ=255)                                                                        gCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGc                                                    >  1:98740/1‑140 (MQ=255)                                                                            caAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGCTTGc                                                >  1:446372/1‑140 (MQ=255)                                                                             aaaTCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGCTTGCg                                               <  1:425235/140‑1 (MQ=255)                                                                                  ggctggctGAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGGATATAACCCGGAGGCCAGCTTGCGGatca                                          >  1:186165/1‑140 (MQ=255)                                                                                        ctgAACCACCGGCATCGACACCTCGATCGCACCGGCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGCTTGCGGATCATCCCgg                                    <  1:310961/140‑1 (MQ=255)                                                                                                                          gCGGTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGCTTGCGGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGc  <  2:446372/140‑1 (MQ=255)                                                                                                                             |                                                                                                                                         GAACGGACGCTCGCCACGGTCAACAAAACGCAGCAGTTCCGGACCGTACTGTTCCCAACGACCACTCTCTTGCCACAAATCGGCTGGCTGAACCACCGGCATCGACACCTCGATCGCACCGGCGTTGTTCATCTCTTCACGCACGATGTTTTCGACTTTTTTCAGAACGCGCACGCCGGTCGGCAGCCAGGTATATAACCCGGAGGCCAGCTTGCGGATCATCCCGGCGCGCAGCATCAGCTGATGGCTGATCACCTCGGC  >  NC_000913/218464‑218724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑