Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,023,670	A→G	F72L (TTC→CTC)	yccS ←	putative transporter YccS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,023,670	0	A	G	100.0%	96.7 / NA	31	F72L (TTC→CTC)	yccS	putative transporter YccS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/18);  total (13/18)

AAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTG  >  NC_000913/1023534‑1023798                                                                                                                                         |                                                                                                                                 aaGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAagcagagcag                                                                                                                               >  2:35552/1‑140 (MQ=255)    gCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGt                                                                                                                            >  1:416204/1‑140 (MQ=255)    gCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGt                                                                                                                            >  1:454927/1‑140 (MQ=255)          gttgCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAatgat                                                                                                                      <  2:426686/140‑1 (MQ=255)                     ttGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCa                                                                                                           >  1:216624/1‑140 (MQ=255)                     ttGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCa                                                                                                           <  2:401936/140‑1 (MQ=255)                     ttGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCa                                                                                                           >  1:114585/1‑140 (MQ=255)                             gACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTccc                                                                                                   >  1:63861/1‑140 (MQ=255)                                 gccgAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCa                                                                                               <  1:53604/140‑1 (MQ=255)                                  ccgAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAg                                                                                              >  1:65751/1‑140 (MQ=255)                                     aGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCg                                                                                           <  1:241128/140‑1 (MQ=255)                                          aaTGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCAt                                                                                      <  2:445518/140‑1 (MQ=255)                                                    cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCg                                                                                           >  1:72262/1‑125 (MQ=255)                                                    cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCg                                                                                           <  2:72262/125‑1 (MQ=255)                                                    cTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGt                                                                            >  1:317548/1‑140 (MQ=255)                                                         agagagCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAgcg                                                                       <  1:35552/140‑1 (MQ=255)                                                          gagagCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAgcgc                                                                      <  1:127126/140‑1 (MQ=255)                                                               cGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTg                                                                 >  2:307983/1‑140 (MQ=255)                                                                   aaGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAACCGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccac                                                             <  2:416204/140‑1 (MQ=255)                                                                   aaGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccac                                                             <  2:454927/140‑1 (MQ=255)                                                                   aaGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccac                                                             <  2:63861/140‑1 (MQ=255)                                                                    aGCCAATCGAAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccacc                                                            <  2:216624/140‑1 (MQ=255)                                                                         aTCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATccc                                                       <  1:153416/140‑1 (MQ=255)                                                                                      agggccagggAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccacca                                                           <  2:56373/123‑1 (MQ=255)                                                                                      agggccagggAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGccacca                                                           >  1:56373/1‑123 (MQ=255)                                                                                                        aTTCTACTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCa                        >  1:246643/1‑140 (MQ=255)                                                                                                              cTGGGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGc                  <  2:503282/140‑1 (MQ=255)                                                                                                              cTGAGGCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGc                  <  1:277718/140‑1 (MQ=255)                                                                                                                   gCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGccacca             >  2:259090/1‑140 (MQ=255)                                                                                                                   gCCGAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGccacca             >  2:468071/1‑140 (MQ=255)                                                                                                                      gAGGCGATAAAAAAGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACgg          <  2:114585/140‑1 (MQ=255)                                                                                                                              aaaaaaGCAGAGCAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCtg  <  1:259090/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                 AAGGCAATTGTTGCATAGCGTTGACCCAGACCGCCGAGCAAAATGAAGCCGCTGGTAGAGAGCGTTAAGCCAATCGCAAATAGCCAGGGCCAGGGAAACAGCAATTCTACTGAGGCCGAGGCGATAAAAAAGCAGAACAGCGTAATGATGAGGTTACGCAAACGTCCCGCCAGTCGGTCATCGAGATCGGTCAGCGCCGCTGCCACCATCCCCAGCGTTAGCGGAATCGTCAGTTTTACATCACCCAGCCACCACGGAAACGCTG  >  NC_000913/1023534‑1023798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑