Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,130,841 A→G V8A (GTG→GCG)  flgA ← flagellar basal body P‑ring formation protein FlgA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,130,8410AG100.0% 153.9 / NA 44V8A (GTG→GCG) flgAflagellar basal body P‑ring formation protein FlgA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

TAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAA  >  NC_000913/1130705‑1130973
                                                                                                                                        |                                                                                                                                    
tagATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCgcgc                                                                                                                                   >  2:539057/1‑140 (MQ=255)
 agATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCt                                                                                                                                  <  1:80367/140‑1 (MQ=255)
   aTTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTAc                                                                                                                                <  1:425314/140‑1 (MQ=255)
       ggCGCTGTACGAATAGCAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCt                                                                                                                                  <  1:384409/134‑1 (MQ=255)
       ggCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCt                                                                                                                                  >  2:384409/1‑134 (MQ=255)
       ggCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGttt                                                                                                                            >  1:164577/1‑140 (MQ=255)
       ggCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGttt                                                                                                                            >  2:21323/1‑140 (MQ=255)
       ggCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGttt                                                                                                                            >  1:272415/1‑140 (MQ=255)
       ggCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGttt                                                                                                                            >  1:280753/1‑140 (MQ=255)
            tGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTttatta                                                                                                                       >  2:442075/1‑140 (MQ=255)
              tACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATc                                                                                                                     >  2:348051/1‑140 (MQ=255)
               aCGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCa                                                                                                                    <  1:103022/140‑1 (MQ=255)
                      gAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCAtttt                                                                                                             >  1:253405/1‑140 (MQ=255)
                        aaCACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCg                                                                                                           >  1:105825/1‑140 (MQ=255)
                          cacGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGcc                                                                                                         <  2:105825/140‑1 (MQ=255)
                              aaCCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCa                                                                                                     <  2:280753/140‑1 (MQ=255)
                                ccTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGc                                                                                                   <  2:164577/140‑1 (MQ=255)
                                ccTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGc                                                                                                   <  1:389343/140‑1 (MQ=255)
                                 cTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGcc                                                                                                  >  2:122232/1‑140 (MQ=255)
                                 cTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGc                                                                                                   >  2:407288/1‑139 (MQ=255)
                                 cTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGc                                                                                                   <  1:407288/139‑1 (MQ=255)
                                  tcatcaCTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCa                                                                                                 <  1:21323/140‑1 (MQ=255)
                                       aCTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCt                                                                                            <  1:442075/140‑1 (MQ=255)
                                             ccccGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACg                                                                                      <  2:214398/140‑1 (MQ=255)
                                                cgcgAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCa                                                                                                     <  1:192829/122‑1 (MQ=255)
                                                cgcgAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCa                                                                                                     >  2:192829/1‑122 (MQ=255)
                                                cgcgAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGa                                                                                   >  2:363490/1‑140 (MQ=255)
                                                cgcgAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGa                                                                                   >  2:49124/1‑140 (MQ=255)
                                                  cgAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAAt                                                                                 >  1:521134/1‑140 (MQ=255)
                                                      ttGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTa                                                                             <  1:122232/140‑1 (MQ=255)
                                                       tGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTAc                                                                            <  1:206466/140‑1 (MQ=255)
                                                                         tgCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATc                                                          <  1:348051/140‑1 (MQ=255)
                                                                          gCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCa                                                         <  1:363490/140‑1 (MQ=255)
                                                                          gCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCACAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCa                                                         <  1:42729/140‑1 (MQ=255)
                                                                            aaTTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAAc                                                       <  1:49124/140‑1 (MQ=255)
                                                                                        agaTTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGc                                           >  2:117384/1‑140 (MQ=255)
                                                                                                   cGTACTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTg                                >  1:253872/1‑140 (MQ=255)
                                                                                                       cTTAACGGACTGAACAGTATCGCGATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGtt                            >  1:521391/1‑140 (MQ=255)
                                                                                                                           cgcgATGATCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAAcgcgcg        >  2:314083/1‑140 (MQ=255)
                                                                                                                               atgatCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAAcgcgcgcg      >  1:37752/1‑138 (MQ=255)
                                                                                                                               atgatCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAAcgcgcgcg      <  2:37752/138‑1 (MQ=255)
                                                                                                                                tgatCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTaa   >  2:151246/1‑140 (MQ=255)
                                                                                                                                 gatCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTaaa  <  1:151246/140‑1 (MQ=255)
                                                                                                                                 gatCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTaaa  <  1:539057/140‑1 (MQ=255)
                                                                                                                                 gatCGCCGCGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTaaa  <  1:117384/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TAGATTGGGCGCTGTACGAATAGAAACACGAACCTCATCACTTACCCCCGCGAGTTGGGCGCTAAAAAAGTTGTGCAATTGCGATGTGAGATTGCTCGCCGTACTTAACGGACTGAACAGTATCGCGATGATCGCCACGCTACGTTTTATTATCAGCATTTTCGCCCCCAGCCATTTCTACAACGTGAATTGTACCTGTCCGCAATGACCATCAACGGCATAAATAGCGACCCATTTTGCGTTTATTCCGCCGATAACGCGCGCGTAAA  >  NC_000913/1130705‑1130973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑