Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,263,889	T→C	V59A (GTT→GCT)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,263,889	0	T	C	100.0%	82.3 / NA	24	V59A (GTT→GCT)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/14);  total (10/14)

GCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTG  >  NC_000913/1263764‑1264023                                                                                                                              |                                                                                                                                       gCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACa                                                                                                                          >  1:45658/1‑140 (MQ=255)          tgtATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAag                                                                                                                 >  1:283081/1‑140 (MQ=255)            tATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAagag                                                                                                               >  2:454099/1‑140 (MQ=255)                 ttttCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGtt                                                                                                          <  2:152032/140‑1 (MQ=255)                               gCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCttt                                                                                            <  1:260784/140‑1 (MQ=255)                               gCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCttt                                                                                            <  1:454099/140‑1 (MQ=255)                                    aTCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGAt                                                                                       >  1:369170/1‑140 (MQ=255)                                      cAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTa                                                                                     >  2:142474/1‑140 (MQ=255)                                            cTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACaa                                                                               <  1:517083/140‑1 (MQ=255)                                             ttGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAAt                                                                              <  2:283081/140‑1 (MQ=255)                                                 cAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTa                                                                          <  2:82553/140‑1 (MQ=255)                                                  aGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTaa                                                                         <  1:75136/140‑1 (MQ=255)                                                  aGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTCATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTGTCACAATCTTaa                                                                         <  2:473084/140‑1 (MQ=255)                                                                          cAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGTGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCct                                                 <  2:236315/140‑1 (MQ=255)                                                                           aGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCctc                                                >  2:179287/1‑140 (MQ=255)                                                                                  cgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc                                         >  1:59716/1‑140 (MQ=255)                                                                                  cgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc                                         >  1:337826/1‑140 (MQ=255)                                                                                  cgTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGc                                         >  1:141886/1‑140 (MQ=255)                                                                                    tggtgCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGGTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCAt                                       <  1:151675/140‑1 (MQ=255)                                                                                                     aaCCGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGt                      <  2:246108/140‑1 (MQ=255)                                                                                                       ccGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTa                    <  2:141886/140‑1 (MQ=255)                                                                                                       ccGCACGGAACTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTa                    <  2:369170/140‑1 (MQ=255)                                                                                                                aCTTTATCTTAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTaa           <  2:45658/140‑1 (MQ=255)                                                                                                                         tAGCGCTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTg  >  2:516173/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                       GCGAGAACGTGTATCGTTTTCGCCGGATAAGCTCGATCAGGCGCTTGACAGCCTGCTTGCGCAGCCGATGGTGCAGGGCGGCGTGGTGCTGTCGACGTGCAACCGCACGGAACTTTATCTTAGCGTTGAAGAGCAGGACAACCTGCAAGAGGCGTTAATCCGCTGGCTTTGCGATTATCACAATCTTAATGAAGAAGATCTGCGTAAAAGCCTCTACTGGCATCAGGATAACGACGCGGTTAGCCATTTAATGCGTGTTG  >  NC_000913/1263764‑1264023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑