Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	241,205	A→G	V700A (GTG→GCG)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	241,205	0	A	G	100.0%	127.2 / NA	37	V700A (GTG→GCG)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/19);  total (18/19)

ATCCGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAAT  >  NC_000913/241079‑241331                                                                                                                               |                                                                                                                               aTCCGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCtt                                                                                                                   >  2:380545/1‑140 (MQ=255) aTCCGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCtt                                                                                                                   >  1:308173/1‑140 (MQ=255)   ccGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCg                                                                                                                 <  1:130222/140‑1 (MQ=255)    cGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGc                                                                                                                <  1:380545/140‑1 (MQ=255)    cGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGc                                                                                                                >  2:394059/1‑140 (MQ=255)         atgaATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACtt                                                                                                           <  2:477175/140‑1 (MQ=255)           gaATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTa                                                                                                         <  2:128042/140‑1 (MQ=255)                      gcggcAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAg                                                                                                 >  1:62569/1‑137 (MQ=255)                      gcggcAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAg                                                                                                 <  2:62569/137‑1 (MQ=255)                                 aTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTg                                                                                   >  2:438175/1‑140 (MQ=255)                                 aTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTg                                                                                   <  2:471624/140‑1 (MQ=255)                                 aTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTg                                                                                   <  2:503986/140‑1 (MQ=255)                                 aTCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTg                                                                                   <  1:394059/140‑1 (MQ=255)                                   ccaccaGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGcc                                                                                 <  2:308173/140‑1 (MQ=255)                                           gcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATg                                                                         >  2:412573/1‑140 (MQ=255)                                           gcCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCAGGAACGGGGGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATg                                                                         >  2:91850/1‑140 (MQ=255)                                                ttCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTc                                                                    <  1:412573/140‑1 (MQ=255)                                                  ccAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCg                                                                  <  1:438175/140‑1 (MQ=255)                                                          ccAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGTCCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGaa                                                          >  1:104911/1‑140 (MQ=255)                                                            aaCCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGa                                                        <  2:260115/140‑1 (MQ=255)                                                              ccGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATc                                                      >  1:397917/1‑140 (MQ=255)                                                                      tGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACAtt                                              >  1:119934/1‑140 (MQ=255)                                                                            ctcgGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTcagcag                                        <  1:91850/140‑1 (MQ=255)                                                                                       gTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCa                             >  1:139222/1‑140 (MQ=255)                                                                                         aCTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCAcg                           <  1:30611/140‑1 (MQ=255)                                                                                           tGACCGCTACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACcgcgcg                         <  2:51818/140‑1 (MQ=255)                                                                                           tGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCAcgcg                         >  2:397676/1‑140 (MQ=255)                                                                                               cgcgACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTc                     >  2:217256/1‑140 (MQ=255)                                                                                                gcgTCCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCg                    <  2:104911/140‑1 (MQ=255)                                                                                                  gACCAATGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCggg                  >  1:313025/1‑140 (MQ=255)                                                                                                       aTGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCAcg                           >  2:391077/1‑126 (MQ=255)                                                                                                       aTGCGGGAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCAcg                           <  1:391077/126‑1 (MQ=255)                                                                                                           gggAACGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGc         <  1:397676/140‑1 (MQ=255)                                                                                                                cGGGTGGCGTTCGGCGCTTGTAAAATCTTCGCCGCTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTaa    <  1:100152/140‑1 (MQ=255)                                                                                                                 gggTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTaaa   >  1:228261/1‑140 (MQ=255)                                                                                                                  ggTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAat  <  1:232276/140‑1 (MQ=255)                                                                                                                  ggTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAat  >  1:208877/1‑140 (MQ=255)                                                                                                                  ggTGGCGTTCGGCGCTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAat  >  2:109682/1‑140 (MQ=255)                                                                                                                               |                                                                                                                               ATCCGCTGATGAATTGGGTCGGCGGCAATCACATCCACCAGCGCCTCTTCCAGCAAGCCAACCGGATTATGCTCGCTCGGCGTCAGGTACTGACCGCGACCAATGCGGGAACGGGTGGCGTTCGGCACTTGTAAAATCTTCGCCACTTTATGATCCAGCTTGTCAGAAGGTGCCAGATAATGACGTCCGGTCGGGAAGATCACCACATTCAGCAGCCCGGCAACCACGCGGTTCGGGAAGTTTTGCAGTAAAT  >  NC_000913/241079‑241331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑