Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,388,876	A→G	intergenic (‑65/‑54)	tpx ← / → ycjG	lipid hydroperoxide peroxidase/L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,388,876	0	A	G	100.0%	78.3 / NA	23	intergenic (‑65/‑54)	tpx/ycjG	lipid hydroperoxide peroxidase/L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/11);  total (12/11)

CGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCG  >  NC_000913/1388749‑1389012                                                                                                                                |                                                                                                                                         cGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTAtt                                                                                                                              <  2:199422/140‑1 (MQ=255)  gCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTAttt                                                                                                                             >  1:274947/1‑140 (MQ=255)    cTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTgc                                                                                                                           >  1:223986/1‑140 (MQ=255)    cTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATA‑AGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTgcg                                                                                                                          >  1:47242/1‑140 (MQ=255)                  tGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATA‑AGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGac                                                                                                            <  2:47242/140‑1 (MQ=255)                    gCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGacc                                                                                                           <  1:334325/140‑1 (MQ=255)                       aCTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGaccacc                                                                                                        <  2:168416/140‑1 (MQ=255)                                       ccTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTa                                                                                        <  2:274947/140‑1 (MQ=255)                                                 aaCGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGaa                                                                              >  1:280700/1‑140 (MQ=255)                                                 aaCGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGaa                                                                              <  2:211501/140‑1 (MQ=255)                                                       ttGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTa                                                                        >  1:82324/1‑140 (MQ=255)                                                                       tttCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGc                                                        <  2:223986/140‑1 (MQ=255)                                                                         tCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCt                                                      <  2:82324/140‑1 (MQ=255)                                                                          ccTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTg                                                     >  2:468631/1‑140 (MQ=255)                                                                                   atatAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACa                                            <  1:374174/140‑1 (MQ=255)                                                                                        gTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATAccc                                       >  1:246563/1‑140 (MQ=255)                                                                                          tAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTgg                                                    >  1:37137/1‑125 (MQ=255)                                                                                          tAACGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTgg                                                    <  2:37137/125‑1 (MQ=255)                                                                                           aaCGTCACACCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGt                                    >  2:227917/1‑140 (MQ=255)                                                                                                 acacCTAGTTTATGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGa                              >  2:123332/1‑140 (MQ=255)                                                                                                             tGCTAACTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGaa                  >  1:326228/1‑140 (MQ=255)                                                                                                                   cTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGa                   >  1:456543/1‑133 (MQ=255)                                                                                                                   cTGTCAATAACACGGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGa                   <  2:456543/133‑1 (MQ=255)                                                                                                                             cacgGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAgcgcg  >  2:326336/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                         CGCCTGCGGGATGGAATTGGCGACTGTAACCGGGTTGCCCTGGAAATGAACGGTTTGTGACATGATTATCTTTCCTGTTTACATATAGTTAACGTCACACCTAGTTTATGCTAACTGTCAATAACACAGCAAACGCTATTTGCGCTTAATCCGCAGACCACCGCGACAACAAGGAGTAAAGATGAGAACCGTTAAGGTATTCGAGGAAGCCTGGCCCTTACATACCCCGTTTGTGATTGCCCGGGGAAGTCGCAGTGAAGCGCG  >  NC_000913/1388749‑1389012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑