Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,532,043	A→G	R76R (AGA→AGG)	ydcC →	H repeat‑associated putative transposase YdcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,532,043	0	A	G	100.0%	57.6 / NA	24	R76R (AGA→AGG)	ydcC	H repeat‑associated putative transposase YdcC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/13);  total (11/13)

TGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCG  >  NC_000913/1531907‑1532177                                                                                                                                         |                                                                                                                                       tGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGtt                                                                                                                                     <  2:43639/140‑1 (MQ=17) tGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGtt                                                                                                                                     <  1:382198/140‑1 (MQ=17)  gTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACGCCATTGCCAGGGTTg                                                                                                                                    <  1:141249/140‑1 (MQ=17)  gTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTg                                                                                                                                    >  1:164890/1‑140 (MQ=17)      aCTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATc                                                                                                                                >  1:268138/1‑140 (MQ=17)          tttGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGt                                                                                                                            >  2:12905/1‑140 (MQ=17)                ccGTTATTTCTGGTGCAGAAGGCTTGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGt                                                                                                                      >  1:81945/1‑140 (MQ=21)                ccGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGt                                                                                                                      >  1:511790/1‑140 (MQ=21)                 cGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTAt                                                                                                                          >  2:133993/1‑135 (MQ=17)                 cGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTAt                                                                                                                          <  1:133993/135‑1 (MQ=17)                 cGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTc                                                                                                                     >  1:245336/1‑140 (MQ=21)                                   aGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGt                                                                                                   <  1:51723/140‑1 (MQ=37)                                    ggCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTg                                                                                                  <  1:99565/140‑1 (MQ=37)                                      cTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCt                                                                                                <  2:267828/140‑1 (MQ=25)                                                          ttGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGAc                                                                            <  2:337772/140‑1 (MQ=21)                                                                aaaCACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCAt                                                                      >  1:326486/1‑140 (MQ=25)                                                                          cGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAgatg                                                            <  1:483375/140‑1 (MQ=17)                                                                                                             gTATTCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTc                         >  2:369780/1‑140 (MQ=17)                                                                                                                ttCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCgg                      <  2:326486/140‑1 (MQ=17)                                                                                                                ttCCTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCCCCgg                      <  2:158051/140‑1 (MQ=17)                                                                                                                  ccTGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCa                    <  2:164890/140‑1 (MQ=17)                                                                                                                    tGTTCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCact                  <  2:81945/140‑3 (MQ=11)                                                                                                                      ttCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCt                >  2:357507/1‑140 (MQ=25)                                                                                                                       tCACGACACCATTGCCAGGGTTGTATCCTGTATCAGTCCTGCAAAAATTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCtt               >  1:229673/1‑140 (MQ=255)                                                                                                                                    gCCAGGGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTcg  <  2:511790/140‑1 (MQ=21)                                                                                                                                         |                                                                                                                                       TGTTGACTATTTGTGCCGTTATTTCTGGTGCAGAAGGCTGGGAAGATATAGAGGATTTTGGGGAAACACATCCCGATTTTTTGAAGCAATATGGTGATTTTGAAAATGGTATTCCTGTTCACGACACCATTGCCAGAGTTGTATCCTGTATCAGTCCTGCAAAATTTCACGAGTGCTTTATTAACTGGATGCGTGACTGCCATTCTTCAGATGATAAAGACGTCATTGCAATTGATGGAAAAACGCTCCGGCATTCTTATGATAAGAGTCG  >  NC_000913/1531907‑1532177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑