Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,634,642	C→T	G82S (GGC→AGC)	tfaQ ←	Qin prophage; putative tail fiber assembly protein TfaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,634,642	0	C	T	100.0%	68.5 / NA	32	G82S (GGC→AGC)	tfaQ	Qin prophage; putative tail fiber assembly protein TfaQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

CCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTT  >  NC_000913/1634511‑1634766                                                                                                                                    |                                                                                                                             ccGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCa                                                                                                                      >  1:222511/1‑140 (MQ=17)    gAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGt                                                                                                                   >  2:371214/1‑140 (MQ=17)          tttttCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATaaa                                                                                                             <  1:513693/140‑1 (MQ=17)               cTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGtt                                                                                                        <  1:183858/140‑1 (MQ=18)                         aTCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgat                                                                                              <  2:36769/140‑1 (MQ=18)                          tCCTTCACCCAGGCTGAGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatg                                                                                             <  2:487477/140‑1 (MQ=16)                              tCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCt                                                                                         <  2:187348/140‑1 (MQ=18)                               cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatga                                                                                            >  1:173841/1‑136 (MQ=17)                               cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCgatga                                                                                            <  2:173841/136‑1 (MQ=18)                               cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCtt                                                                                        >  1:138930/1‑140 (MQ=17)                               cACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCtt                                                                                        >  2:249992/1‑140 (MQ=17)                                      gCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCa                                                                                      >  2:417079/1‑135 (MQ=17)                                      gCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCa                                                                                      <  1:417079/135‑1 (MQ=17)                                          tgCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGc                                                                             >  1:387427/1‑140 (MQ=17)                                                    cTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACt                                                                   >  1:515719/1‑140 (MQ=17)                                                    cTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACt                                                                   <  2:387427/140‑1 (MQ=17)                                                       cTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcat                                                                <  1:480753/140‑1 (MQ=17)                                                         gAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACtcatca                                                              >  2:232254/1‑140 (MQ=17)                                                             cTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGt                                                          >  1:359442/1‑140 (MQ=17)                                                              tCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGtt                                                         <  1:532360/140‑1 (MQ=17)                                                                cccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGa                                                       >  1:63624/1‑140 (MQ=17)                                                                 ccTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGaa                                                      >  1:343844/1‑140 (MQ=17)                                                                               ccAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagc                                        >  1:541186/1‑140 (MQ=17)                                                                                cAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTTCCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcc                                       <  1:479182/140‑1 (MQ=17)                                                                                 aGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagcca                                      <  2:222511/140‑1 (MQ=17)                                                                                    tAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAagccagcc                                   <  1:249992/140‑1 (MQ=17)                                                                                     aaCATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCg                                  <  2:343844/140‑1 (MQ=17)                                                                                             ccGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCt                          >  2:287746/1‑140 (MQ=17)                                                                                                  aaCGGACCGAGTTCAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCgg                     <  2:138930/140‑1 (MQ=17)                                                                                                   aCGGACCGAGTTCAGAAATAAATAACGCGCCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGt                    >  2:423701/1‑140 (MQ=17)                                                                                                               cAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTgg                        >  2:102233/1‑124 (MQ=18)                                                                                                               cAGAAATAAATAACGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTgg                        <  1:102233/124‑1 (MQ=17)                                                                                                                     taaataaCGCGTCGCTGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGttt  <  2:400351/140‑1 (MQ=17)                                                                                                                                    |                                                                                                                             CCGGAACAGTTTTTCTGCTTCCGTATCCTTCACCCAGGCTGTGCCGTTCCACTTCTGAAACTCCCCTTCCGGCGATAACCAGGTAACATTTTCCGGTAACGGACCGAGTTCAGAAATAAATAACGCGTCGCCGGAAGCCACGTCATAAACCGTTTTACCCCGATGATCTTCAACGAGATGCCACGATGACTCATCACTGTTGAAAACAGCCACGAAGCCAGCCGGAATATCTGGCGGTGCAATATCGGTACTGTTT  >  NC_000913/1634511‑1634766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑