Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,691,588	A→G	*422Q (TAA→CAA)	uidC ←	outer membrane porin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,691,588	0	A	G	100.0%	61.9 / NA	19	*422Q (TAA→CAA)	uidC	outer membrane porin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

CCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCT  >  NC_000913/1691453‑1691721                                                                                                                                        |                                                                                                                                      ccTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTc                                                                                                                                   >  1:458912/1‑140 (MQ=255) ccTGTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTTCGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTc                                                                                                                                   <  1:293205/140‑1 (MQ=255)      tCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCAt                                                                                                                              <  2:322586/140‑1 (MQ=255)      tCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCAt                                                                                                                              >  1:494166/1‑140 (MQ=255)       ccTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATa                                                                                                                             <  2:471896/140‑1 (MQ=255)          gCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAg                                                                                                                          <  1:221947/140‑1 (MQ=255)                     aaTTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAAt                                                                                                               >  2:348054/1‑140 (MQ=255)                          aTGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCCGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCa                                                                                                          <  2:494166/140‑1 (MQ=255)                                ttttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTca                                                                                                    <  1:420562/140‑1 (MQ=255)                                  ttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTcaca                                                                                                  <  2:6673/140‑1 (MQ=255)                                   tGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAGGTCATACTGAATACTCATTTTCACAt                                                                                                 <  1:202028/140‑1 (MQ=255)                                                  gCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGc                                                                                  >  2:37802/1‑140 (MQ=255)                                                      ttACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCAC‑TCCCAGTAATTCAGGCGATCg                                                                             >  1:384900/1‑140 (MQ=255)                                                                                                   gAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGc                                               <  1:59460/126‑1 (MQ=255)                                                                                                   gAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGc                                               >  2:59460/1‑126 (MQ=255)                                                                                                          cGGAGATTTTTCTCTCCGGCGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTCTCCCGCCCTTTATCGGCAGCaagaaag                          >  1:285935/1‑140 (MQ=255)                                                                                                                             cGTTATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACg       >  1:397052/1‑140 (MQ=255)                                                                                                                                tATTTTTTGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGcc    <  1:37802/140‑1 (MQ=255)                                                                                                                                  ttttttGCTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCt  >  1:462627/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTCTCCGGCGTTATTTTTTACTTCAGCATAAAGTCATACTGAATACTCATTTTCACATCCCAGTAATTCAGGCGATCGCCCAGCGTACTGCCATCGCGCTTTTCCCGCCCTTTATCGGCAGCAAGAAAGAAATTCATCCCTTGTAACGGCCCT  >  NC_000913/1691453‑1691721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑