Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	243,023	C→T	G94D (GGC→GAC)	fadE ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	243,023	0	C	T	100.0%	89.3 / NA	27	G94D (GGC→GAC)	fadE	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

CGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGCCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCAC  >  NC_000913/242894‑243159                                                                                                                                  |                                                                                                                                         cGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAAt                                                                                                                                <  1:308880/140‑1 (MQ=255)                 ccGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGGTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTg                                                                                                                >  2:484361/1‑140 (MQ=255)                   gggCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTgcg                                                                                                              <  2:2866/140‑1 (MQ=255)                      ccGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGa                                                                                                           >  1:91851/1‑140 (MQ=255)                       cGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGAc                                                                                                          <  1:484361/140‑1 (MQ=255)                         tCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACAt                                                                                                        >  2:41746/1‑140 (MQ=255)                         tCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACAt                                                                                                        >  2:238366/1‑140 (MQ=255)                                 cGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCa                                                                                                <  2:116613/140‑1 (MQ=255)                                   cTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATc                                                                                              <  2:93901/140‑1 (MQ=255)                                                  tCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACcg                                                                               >  1:293648/1‑140 (MQ=255)                                                                        aTGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAatca                                                         <  1:452183/140‑1 (MQ=255)                                                                            aGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGa                                                     >  2:433737/1‑140 (MQ=255)                                                                              cTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACt                                                   >  1:501431/1‑140 (MQ=255)                                                                              cTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACt                                                   >  1:8687/1‑140 (MQ=255)                                                                                ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTa                                                 >  1:318503/1‑140 (MQ=255)                                                                                ttttCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTa                                                 >  2:407721/1‑140 (MQ=255)                                                                                    ccAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCa                                             <  1:41746/140‑1 (MQ=255)                                                                                    ccAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCa                                             <  1:238366/140‑1 (MQ=255)                                                                                    ccAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCAACTTACGCa                                             <  1:150053/140‑1 (MQ=255)                                                                                           ggCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGgcgc                                      <  1:88086/140‑1 (MQ=255)                                                                                                      gAACAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATg                           >  2:183526/1‑140 (MQ=255)                                                                                                         cAAGTCGCCCTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCa                        <  2:545339/140‑1 (MQ=255)                                                                                                                cccTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGAt                 >  2:335869/1‑140 (MQ=255)                                                                                                                 ccTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATa                <  2:8687/140‑1 (MQ=255)                                                                                                                 ccTCCCACCAGGTGGTGTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATa                <  2:52444/140‑1 (MQ=255)                                                                                                                            gtggtgTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAgg     <  2:293648/140‑1 (MQ=255)                                                                                                                            gtggtgTCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAgg     <  1:466424/140‑1 (MQ=255)                                                                                                                               gtgtCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCTGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCAc  >  1:111598/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                         CGGCAGGCTTCTTCTACCGGGCCGTCGAGAAACGCTTGCTCTTCGGCGGTCAGGCGCGGCTGCGGATAGTTATGCAGCTTTTTCCAGTCCGGCTTGCCCTGGAACAAGTCGCCCTCCCACCAGGTGGTGCCCGCATCAATCGCTTCTTTCTCAGTGCGCGACATCGGCGGCATCACCTTACGGAAACCGCGAAATACCGGCGCGGAAATCATCGACTTACGCATAGGCGCAAAGTTAAATGGCACGAGGATAATGGCCAGAGGCAC  >  NC_000913/242894‑243159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑