Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,790,331	A→G	V305A (GTT→GCT)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,790,331	0	A	G	100.0%	82.5 / NA	24	V305A (GTT→GCT)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/11);  total (13/11)

CGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAA  >  NC_000913/1790215‑1790461                                                                                                                     |                                                                                                                                   cgTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGcc                                                                                                             >  2:520149/1‑140 (MQ=255)    tATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGa                                                                                                          >  1:275051/1‑140 (MQ=255)                gCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGAc                                                                                                         <  2:277729/129‑1 (MQ=255)                gCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGAc                                                                                                         >  1:277729/1‑129 (MQ=255)                gCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTc                                                                                              >  1:216919/1‑140 (MQ=255)                         tGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAAcgcg                                                                                     <  2:216919/140‑1 (MQ=255)                                 aGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAgg                                                                             <  2:275051/140‑1 (MQ=255)                                   tttCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGtt                                                                           >  2:168042/1‑140 (MQ=255)                                    ttCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTg                                                                          <  1:173934/140‑1 (MQ=255)                                        gCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTa                                                                      >  1:451479/1‑140 (MQ=255)                                                    ctcCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTaa                                                          <  1:388441/140‑1 (MQ=255)                                                       cATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGc                                                       >  1:300188/1‑140 (MQ=255)                                                       cATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGc                                                       >  2:221153/1‑140 (MQ=255)                                                       cATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGc                                                       <  2:357056/140‑1 (MQ=255)                                                         tAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGccc                                                     <  1:221153/140‑1 (MQ=255)                                                           aTGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCtt                                                   <  1:168042/140‑1 (MQ=255)                                                            tGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTtg                                                  <  2:451479/140‑1 (MQ=255)                                                            tGCGTCAACAAAACCTGCTGATAGCTGTCCACGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTtg                                                  >  1:178591/1‑140 (MQ=255)                                                                 caacaaAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTgatgat                                             >  2:365896/1‑140 (MQ=255)                                                                              tgATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACa                                >  2:334497/1‑140 (MQ=255)                                                                                 tAGCTGTCCACGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTTCAAAt                             <  2:178591/140‑1 (MQ=255)                                                                                       tCCAGGGCCTCATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAAcc                       <  1:520149/140‑1 (MQ=255)                                                                                                 caatCAGGGCAACTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGGCGGCGGGTTGATTATTAAACCTGTAACGCCCTTGGGGATCCGAGTGGTTACAAATAAAAACGGGTTTGTaa             >  2:133901/4‑140 (MQ=255)                                                                                                 cATTCAGGGCATCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTaa             >  2:270972/1‑140 (MQ=255)                                                                                                            tCTACGGCAGCAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGaaa  >  1:308791/1‑140 (MQ=255)                                                                                                                     |                                                                                                                                   CGTTATCCTCTTTTTGCTCCGTCATGAAGCCCAGTTTCTGCCGCATCCGTTCTCCATAATGCGTCAACAAAACCTGCTGATAGCTGTCCAGGGCCTCATTCAGGGCATCTACGGCAACAAATGGCGACAATGTCTGCGCCAGACGCTGTAAATTCCACAACGCGACGGCAGGTTGATTATCAAAGCTGTAACGCCCTTGATGATCCGAGTGATTACAAATAAAACCGGGTTCGTAATCATCAAGAAA  >  NC_000913/1790215‑1790461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑