Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,812,949	A→G	S62P (TCT→CCT)	ydjO ←	protein YdjO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,812,949	0	A	G	100.0%	166.8 / NA	47	S62P (TCT→CCT)	ydjO	protein YdjO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/22);  total (25/22)

TTTATACCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTA  >  NC_000913/1812816‑1813085                                                                                                                                      |                                                                                                                                         tttatACCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCAtata                                                                                                                                    <  2:156095/140‑1 (MQ=255)   tataCCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAgg                                                                                                                                  <  2:306805/140‑1 (MQ=255)       ccTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAAt                                                                                                                              <  1:98944/140‑1 (MQ=255)         tctTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCa                                                                                                                            <  1:349074/140‑1 (MQ=255)             aTCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCAtata                                                                                                                        >  1:169055/1‑140 (MQ=255)                  aaTTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTaa                                                                                                                   >  1:300543/1‑140 (MQ=255)                               tAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGc                                                                                                      <  2:411857/140‑1 (MQ=255)                               tAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGc                                                                                                      >  1:267308/1‑140 (MQ=255)                               tAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGc                                                                                                      >  1:525164/1‑140 (MQ=255)                                      tattaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCa                                                                                               >  1:254099/1‑140 (MQ=255)                                      tattaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCa                                                                                               >  1:523078/1‑140 (MQ=255)                                       attaattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCAt                                                                                              >  1:64818/1‑140 (MQ=255)                                          aattattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGc                                                                                           <  2:525164/140‑1 (MQ=255)                                           attattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCa                                                                                          <  2:343411/140‑1 (MQ=255)                                            ttattaCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAg                                                                                         <  2:523078/140‑1 (MQ=255)                                                 aCTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTcaca                                                                                    <  1:193448/140‑1 (MQ=255)                                                     tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCAt                                                                                              <  2:39857/126‑1 (MQ=255)                                                     tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCAt                                                                                              >  1:39857/1‑126 (MQ=255)                                                     tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGa                                                                                <  2:300543/140‑1 (MQ=255)                                                     tataTTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGa                                                                                >  2:224507/1‑140 (MQ=255)                                                       tatTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGAcc                                                                              >  1:76792/1‑140 (MQ=255)                                                         tttAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCg                                                                            <  2:254099/140‑1 (MQ=255)                                                            aaCATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGtag                                                                         >  2:503498/1‑138 (MQ=255)                                                            aaCATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTg                                                                         >  1:129094/1‑140 (MQ=255)                                                               aTTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGata                                                                      >  2:289282/1‑140 (MQ=255)                                                                 tGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCg                                                                            >  1:350954/1‑132 (MQ=255)                                                                 tGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCg                                                                            <  2:350954/132‑1 (MQ=255)                                                                 tGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGAtata                                                                    >  1:117038/1‑140 (MQ=255)                                                                 tGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGAtata                                                                    >  1:328564/1‑140 (MQ=255)                                                                   tCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaa                                                                  >  2:416552/1‑140 (MQ=255)                                                                   tCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaa                                                                  >  1:246910/1‑140 (MQ=255)                                                                    ccTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaag                                                                 <  1:26707/140‑1 (MQ=255)                                                                        cAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATaaagaaag                                                             >  2:172442/1‑140 (MQ=255)                                                                            tatGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtgag                                                         <  2:328564/140‑1 (MQ=255)                                                                             atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtgagt                                                        <  2:76792/140‑1 (MQ=255)                                                                             atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtgagt                                                        >  1:131724/1‑140 (MQ=255)                                                                             atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtgagt                                                        >  2:507525/1‑140 (MQ=255)                                                                             atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtgagt                                                        >  2:496184/1‑140 (MQ=255)                                                                             atGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAagtgagt                                                        >  2:143067/1‑140 (MQ=255)                                                                                 aTCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTg                                                    <  1:526047/140‑1 (MQ=255)                                                                                 aTCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTg                                                    <  2:147316/140‑1 (MQ=255)                                                                                                    gcTCCTGGCTTTGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCct                                 <  1:496184/140‑1 (MQ=255)                                                                                                              ttGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTaaaa                       <  2:444574/140‑1 (MQ=255)                                                                                                               tGAGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAag                      <  2:129094/140‑1 (MQ=255)                                                                                                                 aGGCATCATAGTTAATGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAagag                    >  1:244374/1‑140 (MQ=255)                                                                                                                              aaTGTTAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGc       >  1:353721/1‑140 (MQ=255)                                                                                                                                   tAGGCATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTa  <  1:172442/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTTATACCTCTTATCCGAATTTCACTACCATAAAGATTATTAATTATTACTCTATATTTAACATTGTCCTGCAGATATGTATCAAAACTAAGTGCTGGCGCTCCTGGCTTTGAGGCATCATAGTTAATGTTAGACATATAGGCAATCATATAATTAAAGTTTGATTCTGCTATTTCATCGCAGTCACATTGACCCGTTGATATAAAGAAAGTGAGTGCTGTCATAGTAGACTGCAGCCTTTCTTTAAAAGAGTCGAAAGCCAGGCTTTTA  >  NC_000913/1812816‑1813085

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑