Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,844,343	T→C	S177G (AGC→GGC)	ynjI ←	DUF1266 domain‑containing protein YnjI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,844,343	0	T	C	100.0%	133.6 / NA	38	S177G (AGC→GGC)	ynjI	DUF1266 domain‑containing protein YnjI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

AGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGCCAGGTTCAGGCTTTCGCGGCCAGAACTCCAGCGTT  >  NC_000913/1844208‑1844476                                                                                                                                        |                                                                                                                                      aGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCt                                                                                                                                   >  2:53480/1‑140 (MQ=255)   tCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTc                                                                                                                                 >  2:144946/1‑140 (MQ=255)   tCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTc                                                                                                                                 >  1:402343/1‑140 (MQ=255)     tGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGa                                                                                                                               <  2:402343/140‑1 (MQ=255)     tGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGa                                                                                                                               <  1:5865/140‑1 (MQ=255)      gAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGAt                                                                                                                              <  1:401989/140‑1 (MQ=255)       aGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATa                                                                                                                             >  1:317396/1‑140 (MQ=255)       aGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATa                                                                                                                             >  2:407774/1‑140 (MQ=255)         ggAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATAcc                                                                                                                           >  2:437253/1‑140 (MQ=255)             aaaCAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCAt                                                                                                                       <  1:437253/140‑1 (MQ=255)              aaCAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCAtt                                                                                                                      <  2:317396/140‑1 (MQ=255)              aaCAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCAtt                                                                                                                      <  1:407774/140‑1 (MQ=255)               aCAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTg                                                                                                                     <  1:155627/140‑1 (MQ=255)               aCAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTg                                                                                                                     <  2:273523/140‑1 (MQ=255)                  gCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATc                                                                                                                  <  2:65788/140‑1 (MQ=255)                  gCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATc                                                                                                                  <  1:197961/140‑1 (MQ=255)                      aTTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTCAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCctct                                                                                                              >  1:337854/1‑140 (MQ=255)                                 aaGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTcgcg                                                                                                   <  2:214839/140‑1 (MQ=255)                                     cGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCtt                                                                                               >  1:390601/1‑140 (MQ=255)                                        tAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTa                                                                                            <  1:276509/140‑1 (MQ=255)                                          aCCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGCCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCATCTAAAGACTCGCTTCTTTTACt                                                                                          >  2:358634/1‑140 (MQ=255)                                               ctctGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGa                                                                                     >  1:430328/1‑140 (MQ=255)                                                      gCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATatcgat                                                                              >  1:443279/1‑140 (MQ=255)                                                                  tttGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAgg                                                                  <  2:430328/140‑1 (MQ=255)                                                                  tttGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAgg                                                                  <  2:487041/140‑1 (MQ=255)                                                                     gCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAg                                                               <  2:394868/140‑1 (MQ=255)                                                                     gCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAg                                                               >  2:117782/1‑140 (MQ=255)                                                                                     cTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAg                                               >  1:91982/1‑140 (MQ=255)                                                                                        cATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAg                                               >  2:243037/1‑137 (MQ=255)                                                                                        cATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAg                                               <  1:243037/137‑1 (MQ=255)                                                                                         aTGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTAt                                           >  1:70567/1‑140 (MQ=255)                                                                                         aTGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTAt                                           >  2:534946/1‑140 (MQ=255)                                                                                               gCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGc                                     <  2:390601/140‑1 (MQ=255)                                                                                                ccAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGcc                                    <  1:117782/140‑1 (MQ=255)                                                                                                                             aTAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGCCAGGTTCAGGCTTTCGCGGCCAGAACTCCa       >  2:314869/1‑140 (MQ=255)                                                                                                                              tAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGCCAGGTTCAGGCTTTcg                     <  2:174623/125‑1 (MQ=255)                                                                                                                              tAACTTTCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGCCAGGTTCAGGCTTTcg                     >  1:174623/1‑125 (MQ=255)                                                                                                                                  tttCGCCATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGCCAGGTTCAGGCTTTCGCGGCCAGAACTCCAGCGtt  >  1:146653/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      AGTCTGAGGGAAAAACAGCAAATTTATTCAACAAGGCGATAACCTGCTCTGGGGCTTCCTCCATGTTTGCTTTAAAGGTATTGGCTCCATGGTCGCCAGAAAGAAAATGCTCCATTAAGGCACAATAACTTTCGCTATCTTCGATACCCCATTGATCCTCTAAAGACTCGCGTCTTTTACTTATGATATCGATCGAGTCAAAAGGAAGCACATGATATTGGAAGGTATCTTTGCCAGGTTCAGGCTTTCGCGGCCAGAACTCCAGCGTT  >  NC_000913/1844208‑1844476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑